Metabolic Flux Analysis in Eukaryotic Cells

Methods and Protocols

  • Deepak Nagrath

Part of the Methods in Molecular Biology book series (MIMB, volume 2088)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Christian-Alexander Dudek, Lisa Schlicker, Karsten Hiller
    Pages 17-32
  3. Nina Grankvist, Jeramie D. Watrous, Mohit Jain, Roland Nilsson
    Pages 73-92
  4. Patricia Altea-Manzano, Dorien Broekaert, João A. G. Duarte, Juan Fernández-García, Mélanie Planque, Sarah-Maria Fendt
    Pages 93-118
  5. Diana Széliová, Harald Schoeny, Špela Knez, Christina Troyer, Cristina Coman, Evelyn Rampler et al.
    Pages 119-160
  6. Darshan M. Sivaloganathan, Xuanqing Wan, Mark P. Brynildsen
    Pages 161-188
  7. Sarah McGarrity, Sigurður T. Karvelsson, Ólafur E. Sigurjónsson, Óttar Rolfsson
    Pages 223-269
  8. Vitaly A. Selivanov, Silvia Marin, Josep Tarragó-Celada, Andrew N. Lane, Richard M. Higashi, Teresa W.-M. Fan et al.
    Pages 271-298
  9. Scott Campit, Sriram Chandrasekaran
    Pages 299-313
  10. Kristopher Rawls, Bonnie V. Dougherty, Jason Papin
    Pages 315-330
  11. Marzia Di Filippo, Chiara Damiani, Marco Vanoni, Davide Maspero, Giancarlo Mauri, Lilia Alberghina et al.
    Pages 331-343
  12. André du Toit, Ben Loos, Jan Hendrik S. Hofmeyr
    Pages 345-357
  13. Sabine Peres, Vincent Fromion
    Pages 359-367
  14. Back Matter
    Pages 369-370

About this book


This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases. 


lipid metabolism Dynamic stable-isotope modeling transcriptional regulatory networks pSIRM Exosome-mediated metabolic flux analysis

Editors and affiliations

  • Deepak Nagrath
    • 1
  1. 1.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0158-7
  • Online ISBN 978-1-0716-0159-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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