Methods and Protocols

  • Mark T. Quinn
  • Frank R. DeLeo

Part of the Methods in Molecular Biology book series (MIMB, volume 2087)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Neutrophils and Neutrophil Disorders: Overviews

    1. Front Matter
      Pages 1-1
    2. Harry L. Malech, Frank R. DeLeo, Mark T. Quinn
      Pages 3-10
  3. Neutrophil Isolation

    1. Front Matter
      Pages 31-31
    2. Silvie Kremserova, William M. Nauseef
      Pages 33-42
    3. Daniel W. Siemsen, Liliya N. Kirpotina, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Porter, Benfang Lei et al.
      Pages 43-59
  4. Neutrophil Chemotaxis, Phagocytosis, and Bactericidal Activity

    1. Front Matter
      Pages 77-77
    2. Liliya N. Kirpotina, Douglas J. Kominsky, Mark T. Quinn, Steve D. Swain
      Pages 79-91
    3. Klaudia Szymczak, Margery G. H. Pelletier, Peter C. W. Gaines
      Pages 93-106
    4. Maurice B. Hallett, Jennie S. Campbell, Iraj Laffafian, Sharon Dewitt
      Pages 117-125
    5. Asya Smirnov, Michael D. Solga, Joanne Lannigan, Alison K. Criss
      Pages 127-140
    6. Gaelen Guzman, Fikadu G. Tafesse
      Pages 141-148
    7. Nicholas J. Magon, Heather A. Parker, Louisa V. Ashby, Reuben J. Springer, Mark B. Hampton
      Pages 149-164
  5. Biochemistry, Biology, and Signal Transduction of Neutrophils

    1. Front Matter
      Pages 165-165
    2. Nicole D. Barth, Marc Vendrell, David A. Dorward, Adriano G. Rossi, Ian Dransfield
      Pages 167-190
    3. Maurice B. Hallett, Rhiannon E. Roberts, Sharon Dewitt
      Pages 191-205
    4. Philip P. Ostrowski, Ziv Roth, Sergio Grinstein
      Pages 207-213
    5. Samia Bedouhène, Pham My-Chan Dang, Margarita Hurtado-Nedelec, Jamel El-Benna
      Pages 215-222
    6. Katja Branitzki-Heinemann, Graham Brogden, Maren von Köckritz-Blickwede
      Pages 223-233
    7. Noah Fine, William Khoury, Michael Glogauer
      Pages 235-241
    8. Patrick P. McDonald, Richard D. Ye
      Pages 261-275
    9. Scott D. Kobayashi, Adeline R. Porter, Sarah L. Anzick, Dan E. Sturdevant, Frank R. DeLeo
      Pages 277-298
  6. NADPH Oxidase and Production of Reactive Oxygen Species

    1. Front Matter
      Pages 299-299
    2. Claes Dahlgren, Halla Björnsdottir, Martina Sundqvist, Karin Christenson, Johan Bylund
      Pages 301-324
  7. Analysis of Neutrophil Extracellular Traps

    1. Front Matter
      Pages 413-413
    2. Ulrike Abu-Abed, Volker Brinkmann
      Pages 415-424
    3. Iwona Cichon, Michal Santocki, Weronika Ortmann, Elzbieta Kolaczkowska
      Pages 443-466
  8. Back Matter
    Pages 467-470

About this book


Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophil: Methods and Protocols, chapters retained from previous editions have been updated and include many new approaches. In addition, this third edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI describes how to analyze formation and function of neutrophil extracellular traps. 

Neutrophil: Methods and Protocols, Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.


·         Provides neutrophil protocols that are broadly applicable to leukocyte biology

·         Includes protocols for isolation of neutrophils from humans and animal species

·         Presents an interesting and informative overview of the field of neutrophil biology

·         Facilitates a better understanding of the role of neutrophils in host defense



Molecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists


Venous Blood (3D) Culturing Eosinophils SIGNAL TRANSDUCTION Rap GTPase Extracellular Matrix

Editors and affiliations

  • Mark T. Quinn
    • 1
  • Frank R. DeLeo
    • 2
  1. 1.Department of Microbiology and ImmunologyMontana State UniversityBozemanUSA
  2. 2.Laboratory of Bacteriology, Rocky Mountain Laboratories Division of Intramural Research, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthHamiltonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0153-2
  • Online ISBN 978-1-0716-0154-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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