© 2006

Electron Tomography

Methods for Three-Dimensional Visualization of Structures in the Cell

  • Joachim Frank

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Michael Marko, Chyong-Ere Hsieh, Carmen A. Mannella
    Pages 49-81
  3. Abraham J. Koster, Montserrat Bárcena
    Pages 113-161
  4. Sami S. Brandt
    Pages 187-215
  5. Jose-Maria Carazo, Gabor T. Herman, Carlos O. S. Sorzano, Roberto Marabini
    Pages 217-243
  6. Michael Radermacher
    Pages 245-273
  7. Elmar Zeitler
    Pages 275-305
  8. Pawel A. Penczek, Joachim Frank
    Pages 307-330
  9. Reiner Hegerl, Achilleas S. Frangakis
    Pages 331-352
  10. Achilleas S. Frangakis, Reiner Hegerl
    Pages 353-370
  11. Ming Jiang, Qiang Ji, Xun Wang, Bruce F. McEwen
    Pages 371-399
  12. Achilleas S. Frangakis, Bimal K. Rath
    Pages 401-416
  13. Back Matter
    Pages 441-455

About this book


Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.

This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author's field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.


biology cell cell biology crystal electron microscopy materials science microscopy structural biology tissue

Editors and affiliations

  • Joachim Frank
    • 1
  1. 1.Wadsworth Center and State University of New YorkAlbanyUSA

Bibliographic information