© 2005

Lotus japonicus Handbook

  • Antonio J. Márquez

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Lotus japonicus, A General Introduction

    1. Eloísa Pajuelo, Jens Stougaard
      Pages 3-24
    2. Pedro Díaz, Omar Borsani, Jorge Monza
      Pages 25-37
    3. Pedro Díaz, Jorge Monza, Antonio Márquez
      Pages 39-50
  3. Symbiotic Processes

    1. Makoto Hayashi1, Myra L Tansengco1, Norio Suganuma, Krzysztof Szczyglowski, Lene Krusell, Thomas Ott et al.
      Pages 53-82
    2. Ani Barbulova, Maurizio Chiurazzi
      Pages 83-86
    3. Sonja Kosuta, Thilo Winzer, Martin Parniske
      Pages 87-95
  4. Cell and Molecular Biology Protocols

    1. Clara L Díaz, Helmi R M Schlaman, Herman P Spaink
      Pages 99-109
    2. Mette Grønlund, Adamantia Agalou, Maria C Rubio, Gerda E M Lamers, Andreas Roussis, Herman P Spaink
      Pages 111-122
    3. Catherine Kistner, Manuel Matamoros
      Pages 123-124
    4. Thomas Ott, Lene Krusell, Michael Udvardi
      Pages 125-128
    5. Jillian Perry, Martin Parniske
      Pages 129-131
  5. Functional Genomics

    1. Satoshi Tabata
      Pages 135-138
    2. Thomas Ott, Lene Krusell, Susanne Freund, Michael Udvardi
      Pages 139-148
    3. Sebastian Kloska, Peter Krüger, Joachim Selbig
      Pages 149-154
    4. Guilhem Desbrosses, Dirk Steinhauser, Joachim Kopka, Michael Udvardi
      Pages 165-174
  6. Mutants, Gene-Tagging, and Genetic Analysis

    1. K Judith Webb, Mark Robbins, Trevor L Wang, Martin Parniske, Antonio Márquez
      Pages 177-186
    2. Eloisa Pajuelo
      Pages 187-195

About this book


Legumes are very important plants playing a central role in biological research. They are a key component of sustainable agricultural systems because of symbiotic nitrogen fixation and other beneficial symbiosis with mycorrhizal fungi. Studies on most of the major leguminous crops are hampered by large genome sizes and other disadvantages which have hindered the isolation and characterisation of genes with important roles in legume biology and agriculture. For this reason Lotus japonicus was chosen as a model species for legume research some ten years ago. Since then, many groups around the world have adopted Lotus as a model and have developed numerous resources and protocols to facilitate basic and applied research on this species. This handbook represents the first effort to compile basic descriptions and methods for research in Lotus, including symbiotic processes, cell and molecular biology protocols, functional genomics, mutants, gene tagging and genetic analysis, transformation and reverse genetic analysis, primary and secondary metabolism, and an exhaustive update of the scientific literature available on this plant.


Assimilat DNA Expression RNA enzymes gene expression genetics metabolism mutant nitrogen roots

Editors and affiliations

  • Antonio J. Márquez
    • 1
  1. 1.University of SevilleSpain

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