Lotus japonicus Handbook

  • Antonio J. Márquez

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Lotus japonicus, A General Introduction

    1. Eloísa Pajuelo, Jens Stougaard
      Pages 3-24
    2. Pedro Díaz, Omar Borsani, Jorge Monza
      Pages 25-37
    3. Pedro Díaz, Jorge Monza, Antonio Márquez
      Pages 39-50
  3. Symbiotic Processes

    1. Makoto Hayashi1, Myra L Tansengco1, Norio Suganuma, Krzysztof Szczyglowski, Lene Krusell, Thomas Ott et al.
      Pages 53-82
    2. Ani Barbulova, Maurizio Chiurazzi
      Pages 83-86
    3. Sonja Kosuta, Thilo Winzer, Martin Parniske
      Pages 87-95
  4. Cell and Molecular Biology Protocols

    1. Clara L Díaz, Helmi R M Schlaman, Herman P Spaink
      Pages 99-109
    2. Mette Grønlund, Adamantia Agalou, Maria C Rubio, Gerda E M Lamers, Andreas Roussis, Herman P Spaink
      Pages 111-122
    3. Catherine Kistner, Manuel Matamoros
      Pages 123-124
    4. Thomas Ott, Lene Krusell, Michael Udvardi
      Pages 125-128
    5. Jillian Perry, Martin Parniske
      Pages 129-131
  5. Functional Genomics

    1. Satoshi Tabata
      Pages 135-138
    2. Thomas Ott, Lene Krusell, Susanne Freund, Michael Udvardi
      Pages 139-148
    3. Sebastian Kloska, Peter Krüger, Joachim Selbig
      Pages 149-154
    4. Guilhem Desbrosses, Dirk Steinhauser, Joachim Kopka, Michael Udvardi
      Pages 165-174
  6. Mutants, Gene-Tagging, and Genetic Analysis

    1. K Judith Webb, Mark Robbins, Trevor L Wang, Martin Parniske, Antonio Márquez
      Pages 177-186
    2. Eloisa Pajuelo
      Pages 187-195
    3. Jillian Perry, Tracey Welham, Soizic Cheminant, Martin Parniske, Trevor Wang
      Pages 197-210
    4. Leila Tirichine, José A. Herrera-Cervera, Jens Stougaard
      Pages 211-215
    5. Niels Sandal, Katja Krause, Mirela Frantescu, Simona Radutoiu, Lene Krusell, Lene H Madsen et al.
      Pages 217-232
    6. Leif Schauser, Sreenath Subrahmanyam, Lene Heegaard Madsen, Niels Sandal, Jens Stougaard
      Pages 233-242
    7. Lene Heegaard Madsen, Jens Stougaard
      Pages 243-248
  7. Transformation and Reverse Genetic Analysis

    1. Patrizia Lombari, Emanuela Ercolano, Hicham El Alaoui, Maurizio Chiurazzi
      Pages 251-259
    2. Clara L Díaz, Mette Grønlund, Helmi R M Schlaman, Herman P Spaink
      Pages 261-277
    3. Leila Tirichine, José A. Herrera-Cervera, Jens Stougaard
      Pages 279-284
    4. Simona Radutoiu, Lene H Madsen, Esben B Madsen, Anna Marie Nielsen, Jens Stougaard
      Pages 285-287
    5. Stig Uggerhøj Andersen, Cristina Cvitanich, Mette Grønlund, Hanne Busk, Dorthe Bødker Jensen, Erik Østergaard Jensen
      Pages 289-292
  8. Primary and Secondary Metabolism

    1. Alicia Orea, Purificación Pajuelo, José M Romero, Antonio J Márquez
      Pages 295-313
    2. Antonio J Márquez, Marco Betti, Margarita García-Calderón, Guillermo Estivill, Alfredo Credali, Purificación Pajuelo et al.
      Pages 315-328
    3. Alicia Orea, Ian Prosser, José M Romero, Antonio J Márquez
      Pages 329-340
    4. Nicolas Rispail, Robert Nash, K Judith Webb
      Pages 341-348
    5. Nicolas Rispail, Phillip Morris, K Judith Webb
      Pages 349-354
    6. Alexandra Lanot, Phillip Morris
      Pages 355-361
  9. Lotus Japonicus Literature

    1. Marco Betti, Antonio J Márquez
      Pages 365-381
  10. Back Matter
    Pages 383-384

About this book


Legumes are very important plants playing a central role in biological research. They are a key component of sustainable agricultural systems because of symbiotic nitrogen fixation and other beneficial symbiosis with mycorrhizal fungi. Studies on most of the major leguminous crops are hampered by large genome sizes and other disadvantages which have hindered the isolation and characterisation of genes with important roles in legume biology and agriculture. For this reason Lotus japonicus was chosen as a model species for legume research some ten years ago. Since then, many groups around the world have adopted Lotus as a model and have developed numerous resources and protocols to facilitate basic and applied research on this species. This handbook represents the first effort to compile basic descriptions and methods for research in Lotus, including symbiotic processes, cell and molecular biology protocols, functional genomics, mutants, gene tagging and genetic analysis, transformation and reverse genetic analysis, primary and secondary metabolism, and an exhaustive update of the scientific literature available on this plant.


Assimilat DNA Expression RNA enzymes gene expression genetics metabolism mutant nitrogen roots

Editors and affiliations

  • Antonio J. Márquez
    • 1
  1. 1.University of SevilleSpain

Bibliographic information

Industry Sectors
Consumer Packaged Goods