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Laboratory Animal Research

, Volume 34, Issue 4, pp 185–194 | Cite as

Growing pigs developed different types of diabetes induced by streptozotocin depending on their transcription factor 7-like 2 gene polymorphisms

  • Ching-Fu Tu
  • Chi-Yun Hsu
  • Meng-Hwan Lee
  • Bo-Hui Jiang
  • Shyh-Forng Guo
  • Chai-Ching Lin
  • Tien-Shuh YangEmail author
Open Access
Article
  • 11 Downloads

Abstract

The different polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene promote variances in diabetes susceptibility in humans. We investigated whether these genotypes also promote differences in diabetic susceptibility in commercial pigs. Growing pigs (Landrace, both sex, 50–60 kg) with the C/C (n=4) and T/T (n=5) TCF7L2 genotypes were identified and intravenously injected with streptozotocin (STZ, 40 mg/kg) twice in weekly intervals, then a high-energy diet was offered. Oral glucose tolerance tests, blood analyses and the homeostasis model assessment-insulin resistance (HOMA-IR) index calculations were performed. The animals were sacrificed at the end of 12 weeks of treatment to reveal the pancreas histomorphometry. The results showed that all of the treated pigs grew normally despite exhibiting hyperglycemia at two weeks after the induction. The glycemic level of the fasting or postprandial pigs gradually returned to normal. The fasting insulin concentration was significantly decreased for the T/T carriers but not for the C/C carriers, and the resulting HOMA-IR index was significantly increased for the C/C genotype, indicating that the models of insulin dependence and resistance were respectively developed by T/T and C/C carriers. The histopathological results illustrated a significant reduction in the pancreas mass and insulin active sites, which suggested increased damage. The results obtained here could not be compared with previous studies because the TCF7L2 background has not been reported. Growing pigs may be an excellent model for diabetic in children if the animals are genetically pre-selected.

Keywords

Diabetes mellitus growing pigs streptozotocin high-energy diet transcription factor 7-like 2 genotype 

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Copyright information

© The Author(s) 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://doi.org/creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Ching-Fu Tu
    • 1
  • Chi-Yun Hsu
    • 1
    • 2
  • Meng-Hwan Lee
    • 1
  • Bo-Hui Jiang
    • 3
  • Shyh-Forng Guo
    • 1
  • Chai-Ching Lin
    • 2
  • Tien-Shuh Yang
    • 1
    • 2
    Email author
  1. 1.Division of Animal Technology, Animal Technology LaboratoriesAgricultural Technology Research InstituteHsinchu CityTaiwan, R.O.C.
  2. 2.Department of Biotechnology and Animal ScienceNational Ilan UniversityYilan City, Yilan CountyTaiwan, R.O.C.
  3. 3.Division of Animal Industry, Animal Technology LaboratoriesAgricultural Technology Research InstituteHsinchu CityTaiwan, R.O.C.

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