Table 3

From: Bringing Laboulbeniales into the 21st century: enhanced techniques for extraction and PCR amplification of DNA from minute ectoparasitic fungi

Genus Species Isolate Host COUNTRY: locality Collector Year of collection Preservation Number of thalli used Extraction protocol SSU ITS LSU
Gloeandromyces nycteribiidarum DH619a Streblidae sp. TRINIDAD J.C. Camacho 2014 95 % EtOH 12 thalli Extract-N-Amp (with glycerine)    KT800008
Herpomyces chaetophilus DH602b Periplaneta americana USA: Massachusetts, Cambridge, Kirkland House T.W. Wang 2014 95 % EtOH 10 female thalli Extract-N-Amp (with glycerine) KT800023 KT800039 KT800009
Herpomyces ectobiae MG001 Blattella germanica POLAND: Warsaw M. Gorczak 2014 95 % EtOH piece of antenna with ± 20 adult thalli ISOLATE II Plant DNA Kit without freeze/thaw KT800024 KT800040  
Herpomyces periplanetae DH602c Periplaneta americana USA: Massachusetts, Cambridge, Kirkland House T.W. Wang 2014 95 % EtOH 11 female thalli Extract-N-Amp (with glycerine) KT800025 KT800041 KT800010
Herpomyces stylopygae DE_HerpBL1 Blatta lateralis HUNGARY W.P. Pfliegler 2014 80 % EtOH piece of antenna with ± 30 adult thalli Heat-extraction KT800026 KT800042 KT800011
Hesperomyces virescens DH167e Cycloneda sanguinea sanguinea GUATEMALA: Huehuetenango Dept., La Laguna R.S. Zack 2013 dried 18 adult thalli Extract-N-Amp KT800027   KT800012
Hesperomyces virescens JP353a Olla v-nigrum USA: Georgia, Peach County, USDA-ARS E. Brooks Thompson 2014 95 % EtOH 10 adult thalli QIAamp DNA Micro Kit KT800028 KT800043 KT800013
Hesperomyces virescens DH486c Harmonia axyridis USA: Massachusetts, World’s End peninsula J. Rykken 2006 dried 16 adult thalli Extract-N-Amp (with glycerine) KT800029 KT800044 KT800014
Hesperomyces virescens DH646c Harmonia axyridis GERMANY: Bavaria state, Bereuth S. Tragust 2013 95 % EtOH 2 adult thalli Extract-N-Amp   KT800045 KT800015
Hesperomyces virescens HM497c Harmonia axyridis USA: Georgia, Peach County, USDA-ARS E. Brooks Thompson 2014 95 % EtOH 15 adult thalli Extract-N-Amp (with Hoyer’s medium) KT800030 KT800046 KT800016
Hesperomyces virescens DE_HV01 Harmonia axyridis HUNGARY: Debrecen W.P. Pfliegler 2014 80 % EtOH 9 adult thalli Heat-extraction KT800031 KT800047 KT800017
Hesperomyces virescens MT001 Harmonia axyridis POLAND: Warsaw M. Tischer 2015 95 % EtOH 1 adult thallus ISOLATE II Plant DNA Kit KT800032 KT800048 KT800018
Laboulbenia diopsidis DH468a Diopsidae sp. SIERRA LEONE: Eastern Province, Nemahugoima W. Rossi 2013 100 % EtOH 12 adult thalli Extract-N-Amp KT800033 KT800049 KT800019
Monoicomyces invisibilis MT004 Anotylus sculpturatus POLAND: Warsaw M. Tischer 2015 95 % EtOH 1 adult thallus ISOLATE II Plant DNA Kit KT800034   
Polyandromyces coptosomalis DH313f Phoeacia sp. nov. ECUADOR: Orellana Province, Quito D. Forero 2009 95 % EtOH 7 female and 2 male thalli Extract-N-Amp KT800035   KT800020
Rhachomyces philonthinus TM10446 Philonthus sp. POLAND: Zachodniopomorskie, Lobzany T. Majewski 2004 70 % EtOH ± 15 adult thalli ISOLATE II Plant DNA Kit KT800036   
Rickia wasmannii DE_Rak4 Myrmica scabrinodis HUNGARY: Rakaca A. Tartally 2014 80 % EtOH 30 adult thalli Heat-extraction KT800037 KT800050 KT800021
Zodiomyces vorticellarius MG003 Helochares obscurus POLAND: Warsaw M. Gorczak 2015 95 % EtOH 1 adult thallus ISOLATE II Plant DNA Kit KT800038   KT800022