Russian Agricultural Sciences

, Volume 44, Issue 1, pp 58–63 | Cite as

Influence of Biofertilizers and Liming on Vetch–Oat Mixture Productivity and Change in Sod-Podzolic Soil Microbocenosis

  • A. N. Naliukhin
  • A. A. Zavalin
  • O. V. Siluyanova
  • D. A. Belozerov
Agrochemistry. Soil Science


The influence of modification of granules of organomineral fertilizer (OMF) by BisolbiFit (Bacillus subtilis Ch-13), Phosphatovite (Bacillus mucilaginosus), and PhosphoActiv (Bacillus subtilis Ch-13 + Bacillus mucilaginosus) biopreparations on the productivity of vetch–oat mixture, as well as on changes in the structure of soil microbocenosis, has been studied under the conditions of a two-factor field experiment. The research was carried out on a soddy medium podzolic light loamy soil. The experiment included six versions: control (without fertilizers); N30P30K30; organomineral fertilizer (OMF); OMF + BisolbiFit modifier; OMF + Phosphatovite; OMF + PhosphoActive with liming (pHKCl 5.9) and without liming (pHKCl 5.1). The fertilizers were introduced with equal portions of nitrogen (30 kg a.i./ha). On average, the highest yield increase for the period from 2015 to 2017 (11.7% with application of N30P30K30 and 10.6% with application of unmodified OMF) was obtained in the OMF + BisolbiFit combined with liming version. The use of other modifiers increased the yield of the herbage of vetch–oat mixture only at the trend level. The use of all types of fertilizers increased the content of crude protein by 1.24–3.34% compared to the control. A trend towards an increase in the concentration of phosphorus and potassium in the herbage has been recorded. The highest yield increase per 1 kg of active ingredient (a.i.) of fertilizers was obtained with application of BisolbiFit: 18.0 kg of feed units without liming and 20.7 kg of feed units with liming. The modern method of high throughput sequencing of the 16S ribosomal RNA gene has detected the leading phylums, the share of which in the soil was significantly higher than that of the others: Acidobacteria (7.1–11.5%) and Actinobacteria (13.6–20.4%), Bacterroidetes (7.2–19.3%), Proteobacteria (45.3–56.2%), and Verrucomicrobia (4.3–10.3%). The highest proportion of Actinobacteria (15–20%) was recorded in versions with application of N30P30K30, OMF, and OMF + BiosolbiFit and the highest proportion of the Proteobacteria phylum was recorded in the version with application of OMF + BisolbiFit, which indicates intensive nitrification processes.


vetch–oat mixture organomineral biomodifiers yield profitability of fertilizers soil microbocenosis sod-podzolic soil 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Doran, J.W. and Zeiss, M.R., Soil health and sustainability: Managing biotic component of soil quality, Appl. Soil Ecol., 2000, vol. 15, pp. 3–11.CrossRefGoogle Scholar
  2. 2.
    Semenov, A.M. and Sokolov, M.S., The concept of health of the soil: Fundamental and applied aspects of the justification of evaluation criteria, Agrokhimiya, 2016, no. 1, pp. 3–16.Google Scholar
  3. 3.
    Zavalin, A.A., Tarasov, A.L., Chebotar’, V.K., and Kazakov, A.E., Efficiency of application of ammonium nitrate processed by biopreparation for spring wheat, Vestn. Ross. Akad. S-kh. Nauk, 2008, no. 1, pp. 64–66.Google Scholar
  4. 4.
    Zavalin, A.A., Chernova, L.S., and Gavrilova, A.Yu., Increase of efficiency of mineral fertilizers after their biomodification with BisolbiFit, Plodorodie, 2014, no. 6, pp. 6–8.Google Scholar
  5. 5.
    Tikhonovich, I.A., Kozhemyakov, L.N., and Chebotar’, V.K., Biopreparaty v sel’skom khozyaistve (Metodologiya i praktika primeneniya mikroorganizmov v rastenievodstve i kormoproizvodstve) (Biopreparations in Agriculture (Methodology and Practice of Using Microorganisms in Plant Growing and Forage Production)), Moscow: RASKhN, 2005.Google Scholar
  6. 6.
    Zavalin, A.A., Chebotar’, V.K., Aritkin, A.G., and Esin, V.V. Experience of application of biomineral fertilizers, Mir Sery N P K, 2011, no. 6, pp. 27–30.Google Scholar
  7. 7.
    Naliukhin, A.N., Vlasova, O.A., and Siluyanova, O.V., Efficiency of biological modification of granules of organomineral fertilizers in cultivating agricultural crops, Rol’ molodykh uchenykh v reshenii aktual’nykh zadach APK: Sbornik nauch. trudov mezhd. nauchnoprakt. konf. (The Role of Young Scientists in Solving Urgent Problems of the Agroindustrial Complex: Proc. Sci. Works of Int. Sci.Pract. Conf.), St. Petersburg, 2016, pp. 67–70.Google Scholar
  8. 8.
    Okon, Y. and Labandera-Gonzalez, C., Agronomic applications of Azospirillum: An evaluation of 20 years world-wide field inoculation experiments, Soil Biol. Biochem., 1994, vol. 26, pp. 1591–1601.CrossRefGoogle Scholar
  9. 9.
    Novye tekhnologii proizvodstva i primeneniya biopreparatov kompleksnogo deistviya (New Technologies for the Production and Use of Biologics of Complex Action), Zavalin, A.A. and Kozhemyakov, A.P., Eds., St. Petersburg: Khimizdat, 2010.Google Scholar
  10. 10.
    Naliukhin, A.N., Chukhina, O.V., and Vlasova, O.A., Soils of the Vereshchagin VGMKhA experimental field and their agrochemical characteristics, Molochnokh. Vestn., 2015, no. 3, pp. 35–46.Google Scholar
  11. 11.
    Andronov, E.E., Kutovaya, O.V., and Ivanova, E.A., Perspectives of using data of high-performance sequencing in fundamental and applied soil microbiology, Sovremennye metody issledovanii pochv i pochvennogo pokrova. Mater. Vseros. konf. s mezhdunar. uchastiem (Modern Methods of Soil and Soil Cover Research. Proc. All-Russ. Conf. with Int. Participation), 2015, pp. 36–50.Google Scholar
  12. 12.
    Yu, Y., Lee, Ch., Kim, J., and Hwang, S., Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction, Biotechnol. Bioeng., 2005, vol. 89, no. 6, pp. 670–679.CrossRefPubMedGoogle Scholar
  13. 13.
    Chirak, E.L., Pershina, E.V., Dol’nik, A.S., Kutovaya, O.V., Vasilenko, E.S., Kogut, B.M., Merzlyakova, Ya.V., and Andronov, E.E., The taxonomic structure of microbial communities in soils of different types according to the data of high-performance sequencing of the libraries of the 16S-pRNA gene, S-kh. Biol., 2013, no. 3, pp. 100–109.Google Scholar

Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • A. N. Naliukhin
    • 1
  • A. A. Zavalin
    • 2
  • O. V. Siluyanova
    • 1
  • D. A. Belozerov
    • 1
  1. 1.Vereshchagin Vologda State Dairy AcademyVologdaRussia
  2. 2.Pryanishnikov All-Russia Research Institute of AgrochemistryMoscowRussia

Personalised recommendations