Study of the interaction of antigens and antibodies by photoluminescence spectra excited by ultraviolet laser radiation
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Photoluminescence spectra of isotonic solutions of antigens, i.e., “Taeniarhynchus saginatus” (polysaccharide) larvae and aqueous solutions of antibodies to them, extracted from calf blood serum are measured. Photoluminescence spectra were excited by ultraviolet radiation (266 nm) of the fourth harmonic of the yttrium aluminum garnet laser. The spectra of these objects are strong bands with intensity maxima at wavelengths of 341 and 332 nm for serum and polysaccharide, respectively. It was found that mixing of polysaccharide and serum solutions in a ratio 1:6 results in a drastic decrease in the photoluminescence intensity spectrum, which indicates bonding of corresponding molecular structures.
Keywordsphotoluminescence antibodies antigens albumin metabolism polysaccharide serum spectrum
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