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Evaluation of the Genes Expression Related to the Immune System in Response to Helicobacter pylori Catalase Epitopes

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Abstract

Helicobacter pylori is the main cause of gastritis, peptic ulcers, gastric mucosa-associated lymphoid tissue lymphoma (MALT) and gastric cancer. H. pylori Infects more than half of the world’s population. Antibiotic treatments are not ideal because of antibiotic side effects, re-infection and increased antibiotic resistance. Vaccination against H. pylori is considered as an alternative with the suitable cost for eradication therapy. Th1 immune response plays an important role in vaccine-induced protection against H. pylori infection. An effective vaccine should be able to change the immune balance in favor of the Th1 immune response. Epitope vaccines are believed to elicit more specific and safe immune responses than other vaccines against Helicobacter pylori infection. Catalase is one of the most important immunogenic antigens of H. pylori. We found that the seven epitopes of H. pylori catalase can produce a significant Th1responses by expressing IFN-γ. A decrease in regulation levels of IL-4 was observed after stimulation with catalase epitopes. These results confirm strong response of Th1 immune to H. pylori compared to Th2 immune responses. Understanding of cellular immunity against H. pylori infection guides us to design of H. pylori appropriate vaccines better.

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Funding

Ahvaz Jundishapur University of Medical Sciences has funded our research.

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Authors and Affiliations

  1. Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Manoochehr Makvandi & Niloofar Neissi

  2. Alimentary Tract Research Center, Imam Khomeini Hospital Clinical Research Development Unit, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Manoochehr Makvandi

  3. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Parastoo Tarighi

  4. Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Kimia Makvandi

  5. Department of Medical Laboratory Sciences, School of Para Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Niloufar Rashidi

  6. Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Niloufar Rashidi

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  1. Manoochehr Makvandi
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  2. Niloofar Neissi
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  3. Parastoo Tarighi
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  4. Kimia Makvandi
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  5. Niloufar Rashidi
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Correspondence to Niloufar Rashidi.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Manoochehr Makvandi, Neissi, N., Tarighi, P. et al. Evaluation of the Genes Expression Related to the Immune System in Response to Helicobacter pylori Catalase Epitopes. Mol. Genet. Microbiol. Virol. 35, 47–51 (2020). https://doi.org/10.3103/S089141682001005X

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  • DOI: https://doi.org/10.3103/S089141682001005X

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