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New Bacterial Strains of Рseudomonas laurentiana: Promising Agents for Agrobiotechnology

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Abstract

Bacterial strains ANT 17 and ANT 56, antagonistic to plant pathogenic fungi Bipolaris sorokiniana, were isolated from activated sludge. Physiological, biochemical, and culture morphological properties and analysis of the 16S rRNA gene sequence and composition of fatty acids of cell walls of strains ANT 17 and ANT 56 supported its classification as the species Pseudomonas laurentiana. It was shown that strains P. laurentiana ANT 17 and P. laurentiana ANT 56 possess a set of properties characteristic of PGP (plant growth-promoting) microorganisms: they exhibit antifungal activity against phytopathogenic micromycetes and are capable of decomposing phosphates and synthesizing phytohormonal substances. Inoculation of cucumber, tomato, and cabbage seeds had a beneficial effect on their germination. Presowing treatment of wheat seeds under conditions of a natural infectious background with an inoculum of the isolated bacterial strains contributed to a decrease in the spread of fungi that cause root rot. The possibility of using strains P. laurentiana ANT 17 and P. laurentiana ANT 56 in biotechnology in order to increase the productivity of agroecosystems is suggested. The ability to stimulate the growth and development of plants for P. laurentiana strains is shown for the first time.

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Funding

This study was carried out using the equipment of the Agidel Regional Center for Shared Use in the framework of the state assignment of Ufa Federal Research Center, Russian Academy of Sciences (no. 075-00326-19-00) within the subject no. AAAA-A18-118022190100-9 at Ufa Institute of Biology (Ufa Institute of Biology, Ufa Federal Research Center, Russian Academy of Sciences).

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Authors and Affiliations

  1. Ufa Institute of Biology, Ufa Federal Research Center, Russian Academy of Sciences, 450054, Ufa, Russia

    G. F. Rafikova, E. V. Kuzina, T. Yu. Korshunova & O. N. Loginov

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  1. G. F. Rafikova
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  2. E. V. Kuzina
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  3. T. Yu. Korshunova
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  4. O. N. Loginov
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Correspondence to G. F. Rafikova.

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The authors declare no conflict of interests. The work was carried out without the use of animals and without the involvement of people as subjects.

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G.F. Rafikova ORCID: https://orcid.org/0000-0001-7655-5588.

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Translated by K. Lazarev

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Rafikova, G.F., Kuzina, E.V., Korshunova, T.Y. et al. New Bacterial Strains of Рseudomonas laurentiana: Promising Agents for Agrobiotechnology. Moscow Univ. Biol.Sci. Bull. 75, 206–211 (2020). https://doi.org/10.3103/S0096392520040082

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  • DOI: https://doi.org/10.3103/S0096392520040082

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