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Patterns of Allelic Diversity in Spring Wheat Populations by SSR-Markers

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Abstract

Precise assessment of diversity in available breeding germplasm helps to preempt epidemics and abrupt environmental changes. Spring wheat germplasm consisting of 84 accessions including cultivars, breeding lines and landraces from various origins was scanned with 44 SSRs. For allele frequencies, allelic patterns, heterozygosity and polymorphism the selected population was divided in three subpopulations: (i) pre-green revolution (pre-1965), (ii) post-green revolution (post-1965), (iii) post-veery (post-2000). Alleles produced in pre-1965, post-1965 and post-2000 subpopulations were 115, 144 and 131, respectively. Mean PIC values for pre-1965, post-1965 and post-2000 subpopulations were 0.48, 0.52 and 051, respectively. Allelic patterns showed no locally common alleles in any of the subpopulation. The pre-1965 subpopulation had also no private allele, however, average number of private alleles decreased from post-1965 to post-2000 subpopulation. In case of effective alleles and Shannon’s information index trend was increasing from pre-1965 to post-1965 and then decreasing from post-1965 to post-2000. The decreasing trend alarms the reduced genetic diversity in wheat varieties developed after 2000. PCA and cluster analysis didn’t clearly differentiated subpopulations, though pre-1965 genotypes showed higher genetic distance from post-1965 and post-2000 subpopulations. The decreasing measures of genetic diversity in post-2000 wheat genotypes should be a concern for wheat breeders, therefore, all sources of broadening genetic diversity should be exploited.

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Authors and Affiliations

  1. Department of Environmental Sciences, COMSATS Institute of Information Technology, Vehari, Pakistan

    Muhammad Sajjad

  2. Center for Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan

    Sultan Habibullah Khan

  3. Agricultural Biotechnological Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan

    Munawar Shahzad

Authors
  1. Muhammad Sajjad
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  2. Sultan Habibullah Khan
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  3. Munawar Shahzad
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Correspondence to Muhammad Sajjad.

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Sajjad, M., Khan, S.H. & Shahzad, M. Patterns of Allelic Diversity in Spring Wheat Populations by SSR-Markers. Cytol. Genet. 52, 155–160 (2018). https://doi.org/10.3103/S0095452718020081

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