Advertisement

Russian Agricultural Sciences

, Volume 44, Issue 5, pp 460–464 | Cite as

Association of Leptin Gene (LEP) Polymorphism with Growth Rates and Milk Production in Holstein First-Calf Heifers

  • N. A. BalakirevEmail author
  • N. Yu. Saphina
  • Yu. R. Yulmeteva
  • Sh. K. Shakirov
  • F. F. Zinnatova
Animal Husbandry
  • 8 Downloads

Abstract

The objective of the work is to study the polymorphic leptin gene variants and their influence on growth, development, and milk production of Holstein first-calf heifers, whose 172 blood samples were analyzed. Genotyping the leptin gene locus was performed with the PCR-RFLP method. The frequencies of occurrence of the C and T alleles comprised 0.62 and 0.38, respectively; the values for genotypes CC, TC, and TT comprised 35.5% (61 animals), 53.3% (90 animals), and 12.2% (21 animals), respectively. It was ascertained that the analyzed animal population is in the genetic balanced equilibrium state according to the Hardy–Weinberg law. Under similar conditions of feeding and nutritional supplement, the animals having genotype CC were significantly superior to their peers with the other genotypes in all the parameters characterizing the live weight at different ages. However, the milk yield, the fat mass fraction, the milk fat yield, and the milk protein yield in the specimens having the TT genotype were higher by 8.9% (P ≤ 0.01; 673.4 kg), 0.20, 12.9% (P ≤ 0.05; 38.4 kg), and 9.0% (P ≤ 0.05; 22.7 KG), respectively, than that in the genotype TC carriers. The coefficient of milkability was 142.1 kg higher in all the first-calf heifers when compared to the lower value for the entire herd, therefore, indicating the specific dairy type of the analyzed animal population. The obtained data can prove the associations between the different genotypes of the leptin gene and the growth rates, the physical development, and the milk production in Holstein cattle.

Keywords

cows Holstein breed gene leptin polymorphism live weight milk yield fat protein 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Korovin, A.V., Karamaev, S.V., and Bakaeva, L.N., Features of the growth and development of dairy heifers under the conditions of the industrial complex, Izv. Orenb. Gos. Agrarn. Univ., 2013, no. 2, pp. 137–140.Google Scholar
  2. 2.
    Kosilov, V.I. and Mironenko, S.I., Formation and implementation of the reproductive function of cattle females of the Red Steppe breed and its hybrids, Vestn. Ross. Akad. S-kh. Nauk, 2010, no. 3, pp. 64–66.Google Scholar
  3. 3.
    Sharafutdinov, G.S., Sibagatullin, F.S., Adzhibekov, K.K., Shaidullin, R.R., et al., Kholmogorskii skot Tatarstana: Evolyutsiya, sovershenstvovanie i sokhranenie genofonda (Kholmogorsky Cattle of Tatarstan: Evolution, Improvement, and Preservation of the Gene Pool), Kazan: Kazan. Univ., 2004.Google Scholar
  4. 4.
    Annenkova, N.V., Productive qualities of dairy cows depending on the intensity of forming into an adult, Nauka I innovatsii v sel’skom khozyaistve (Materialy Mezhdunarodnoi nauchno-prakticheskoi konferentsii) (Science and Innovation in Agriculture (Proc. Int. Sci. Pract. Conf.)), Kursk: Kursk. Gos. S-kh. Akad., 2011, part 3, pp. 27–29.Google Scholar
  5. 5.
    Kolesen’, V.P., Yurashchik, S.V., Deshko, I.A., and Dyuba, M.I., Osnovy razvedeniya sel’skokhozyaistvennykh zhivotnykh (Basics of Animal Husbandry Breeding), Grodno: Grodn. Gos. Agrarn. Univ., 2008.Google Scholar
  6. 6.
    Nkrumah, J.D., Li, C., Yu, J., Hansen, C., Keisler, D.H., and Moore, S.S., Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit, J. Anim. Sci., 2005, vol. 83, pp. 20–28.CrossRefGoogle Scholar
  7. 7.
    Rachkova, E.N., Zinnatova, F.F., Yul’met’eva, Yu.R., Akhmetov, T.M., and Shakirov, Sh.K., Association of TG5 and LEP polymorphism with the dynamics of lactation of first-calf cows, Vet. Vrach, 2016, no. 6, pp. 61–66.Google Scholar
  8. 8.
    Armstrong, E., Peñagaricano, F., Artigas, R., De Soto, L., Corbi, C., Llambí, S., Rincón, G., and Postiglioni, A., Molecular markers related to marbling in Uruguayan Creole cattle, Arch. Zootec., 2011, vol. 60, no. 231, pp. 707–716.CrossRefGoogle Scholar
  9. 9.
    Ye, S., Dhillon, S., Ke, X., Collins, A., and Day, I., An efficient procedure for genotyping single nucleotide polymorphisms, Nucleic Acids Res., 2001, vol. 29, no. 17, p.88.CrossRefGoogle Scholar
  10. 10.
    Kalashnikova, L.A., Dunin, I.M., Glazko, V.I., Ryzhova, N.V., and Golubina, E.P., DNK-tekhnologii otsenki sel’skokhozyaistvennykh zhivotnykh (DNA Technologies for Evaluation of Farm Animals), Lesnye Polyany: Vseross. Nauchno-Issled. Inst. Plemennogo Dela, 1999.Google Scholar
  11. 11.
    Merkur’eva, E.K. and Shangin-Berezovskii, G.N., Genetika s osnovami biometrii (Genetics with Basics of Biometrics), Moscow: Kolos, 1983.Google Scholar
  12. 12.
    Svechin, Yu.K. and Dunaev, L.I., Forecasting milk production of cattle, Zootekhniya, 1989, no. 1, pp. 49–53.Google Scholar

Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • N. A. Balakirev
    • 1
    Email author
  • N. Yu. Saphina
    • 2
    • 3
  • Yu. R. Yulmeteva
    • 2
  • Sh. K. Shakirov
    • 2
  • F. F. Zinnatova
    • 2
  1. 1.Scryabin Moscow State Academy of Veterinary Medicine and BiotechnologyMoscowRussia
  2. 2.Tatar Research Institute of Agriculture, “Kazan Science Center” Federal Research CenterRussian Academy of SciencesKazanRussia
  3. 3.Bauman Kazan State Academy of Veterinary MedicineKazanRussia

Personalised recommendations