Optical Memory and Neural Networks

, Volume 27, Issue 4, pp 292–296 | Cite as

Optical Analysis of Synovial Fluid of Patients with Knee Joint Osteoarthrosis

  • E. V. TimchenkoEmail author
  • P. E. Timchenko
  • L. T. Volova
  • D. A. Dolgushkin
  • V. A. Lazarev
  • E. F. Yagofarova
  • M. D. Markova


The results of the study of synovial fluid (SF) of patients with knee joint osteoarthritis (OA). The optical analysis of the SF samples, harvested during exploratory punctures of knee joints of patients suffering the osteoarthrosis in different stages using the standard method, was made. A certain component composition of surface of SF samples that differs for healthy people and the patients, having knee joint osteoarthrosis in different stages, is possible to identify as a result of analysis with the use of Raman spectroscopy method. The introduced optical coefficients help to estimate the spectral composition of surface of SF samples of the patients, suffering the early and late stages of ОА.


optical analysis Raman spectrometry osteoarthrosis synovial fluid 



The reported study was funded by RFBR according to the research project no. 18-32-00004.


  1. 1.
    Chichasova, N.V., Hyaluronic acid preparations in the treatment of osteoarthritis. Synvisc (Hylan G-F 20): Efficacy and safety, Mod. Rheumatol. J., 2013, no. 4, pp. 85–91.Google Scholar
  2. 2.
    Novikov, V.E., Cartilage protectors, Rev. Clin. Pharmacol. Drug Ther., 2010, vol. 8, no. 4, pp. 41–47.Google Scholar
  3. 3.
    Kupchinov, B.I., Ermakov, S.F., and Beloenko, E.D., Biotribology of Synovial Joints, Minsk: Vedi, 1997.Google Scholar
  4. 4.
    Matveyeva, E.L., Karaseva, T.Y., and Karasev, E.A., The changes in synovial fluid composition of patients with neglected and recent meniscus injuries, Genij Ortop., 2008, no. 3.Google Scholar
  5. 5.
    Kamishnikova, V.S., The Methods of Clinical Laboratory Research: Textbook, Kamishnikov, V.S., Ed., Moscow: Medpress-Inform, 2009, 3rd ed.Google Scholar
  6. 6.
    Chernyakova, J.M. and Sementovskaya, E.A., Synovial Fluid: Composition, Properties, Research Laboratory Methods, 2005, no. 2.Google Scholar
  7. 7.
    Timchenko, P.E., Timchenko, E.V., Pisareva, E.V., Vlasov, M.Yu., Red’kin, N.A., and Frolov, O.O., Spectral analysis of allogeneic hydroxyapatite powders, J. Phys.: Conf. Ser., 2017, vol. 784, no. 1, 012060.Google Scholar
  8. 8.
    Timchenko, E.V., Timchenko, P.E., Lichtenberg, A., Assmann, A., Aubin, H., Akhyari, P., Volova, L.T., and Pershutkina, S.V., Assessment of decellularization of heart bioimplants using a Raman spectroscopy method, J. Biomed. Opt., 2017, vol. 22, no. 9, 091511.CrossRefGoogle Scholar
  9. 9.
    Timchenko, E.V., Timchenko, P.E., Volova, L.T., Dolgushkin, D.A., Shalkovsky, P.Y., and Pershutkina, S.V., Detailed spectral analysis of decellularized skin implants, J. Phys.: Conf. Ser., 2016, vol. 737, 012050, pp. 1–4.Google Scholar
  10. 10.
    Esmonde-White, K.A., Raman Spectroscopy Detection of Molecular Changes Associated with Osteoarthritis, 2009.Google Scholar
  11. 11.
    Esmonde-White, K.A., Mandair, G.S., Raaii, F., Roessler, B.J., and Morris, M.D., Raman spectroscopy of dried synovial fluid droplets as a rapid diagnostic for knee joint damage, Biomed. Opt. Spectrosc., 2008, vol. 6853. doi 10.1117/12.770360Google Scholar
  12. 12.
    Ellis, I.D., Cowcher, P.D., Ashton, L., O’Hagan, S., and Goodacrea, R., Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool, Analyst, 2013, vol. 138, no. 14, pp. 3847–4204.CrossRefGoogle Scholar
  13. 13.
    Esmonde-White, K.A., Mandair, G.S., Esmonde-White, F.W.L., Raaii, F., Roessler, B.J., and Morris, M.D., Osteoarthritis screening using Raman spectroscopy of dried human synovial fluid drops, Opt. Bone Biol. Diagn., 2009, vol. 7166. doi 10.1117/12.810057Google Scholar
  14. 14.
    Esmonde-White, K.A., Mandair, G.S., Raaii, F., Jacobson, J.A., Miller, B.S., Urquhart, A.G., Roessler, B.J., and Morris, M.D., Raman spectroscopy of synovial fluid as a tool for diagnosing osteoarthritis, J. Biomed. Opt., 2009, vol. 14, no. 3, 034013. doi 10.1117/1.3130338CrossRefGoogle Scholar
  15. 15.
    Stetsyura, I.Y., Remotely moved sensors on the basis of the giant Raman scattering effect for invitro research, Cand. Sci. (Phys.-Math.) Dissertation, Saratov, 2016.Google Scholar
  16. 16.
    Matveyeva, E.L., Karmatskih, O.L., Talashova, I.A., Makushin, V.D., Chegurov, O.K., and Soldatov, Y.P., Clinical importance of studying albumin spectrum of synovial fluid (Preliminary report), Genij Ortop., 2000, no. 1, pp. 69–71.Google Scholar
  17. 17.
    Timchenko, E.V., Timchenko, P.E., Volova, L.T., Ponomareva, Yu.V., and Taskina, L.A., Raman spectroscopy of the organic and mineral structure of bone grafts, Quantum Electron., 2014, vol. 44, no. 7, pp. 696–699.CrossRefGoogle Scholar

Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • E. V. Timchenko
    • 1
    Email author
  • P. E. Timchenko
    • 1
  • L. T. Volova
    • 2
  • D. A. Dolgushkin
    • 2
  • V. A. Lazarev
    • 2
  • E. F. Yagofarova
    • 1
  • M. D. Markova
    • 1
  1. 1.Samara National Research UniversitySamaraRussia
  2. 2.Samara State Medical UniversitySamaraRussia

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