Identification of Markers for Dengue Infection in Patients after Visiting Dengue-Endemic Countries

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In studied blood serum samples from Russian patients hospitalized with suspected dengue fever, one or more markers of dengue fever have been detected, namely, viral RNA, NS1 antigen, and IgM or IgG antibodies. Moreover, only the combined use of various immunological and genetic methods provided a more effective diagnosis of dengue fever. The detection of IgG to dengue virus in patients with high titers of IgG to tick-borne encephalitis virus (TBEV) and, conversely, the detection of IgG to TBEV virus in patients with high titers of IgG to dengue has been documented. The results confirm the literature data on pronounced antigenic cross-sections between different representatives of flaviviruses, which may complicate the ELISA diagnosis of TBEV and dengue in patients. Genotyping of obtained dengue virus isolates showed that imported cases of dengue fever are caused by subtypes 1–4 of the dengue virus, with the prevalence of infection cases caused by dengue virus 1. Sequencing of these dengue virus isolates, including genome-wide, showed that viral isolates obtained on the territory of Russia are phylogenetically close to modern variants of the dengue virus circulating in the countries of Southeast Asia.

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  1. 1

    Dengue Guidelines for Diagnosis, Treatment, Prevention and Control. WHO Library Cataloguing-in-Publication Data, TDR/World Health Organization, 2009.

  2. 2

    Ong, S.H., Molecular Epidemiology of Dengue Viruses from Complete Genome Sequences, PhD Thesis, Univ. of Basel, 2010.

  3. 3

    Berillo, S.A., Demina, O.K., Ternovoi, V.A., Shikov, A.N., Sergeeva, E.I., Demina, A.V., Kostina, N.E., Vinokurova, A.V., Mikheev, V.N., Agafonov, A.P., and Sergeev, A.N., Cases of Dengue fever in the Russian Federation in travelers, returning from Southeast Asia in 2010–2011, Epidemiol. Infect. Dis., 2012, no. 2, pp. 12–15.

  4. 4

    Khokhlova, N.I., Krasnova, E.I., Vereikina, O.A., Esikova, E.Yu., Pozdnyakova, L.L., Paturina, N.G., Berillo, S.A., Sementsova, A.O., Chausov, E.V., and Ternovoi, V.A., Clinical-laboratory characteristic of imported cases of Dengue fever in Novosibirsk citizens, Infekts.Bolezni, 2013, vol. 11, no. 2, pp. 81–85.

  5. 5

    P'yankov, S.A., Ivanova, E.V., Ternovoi, V.A., and Agafonov, A.P., Screening of blood serum for markers of infection with measles, rubella, cytomegalovirus and Dengue fever in Russian tourists, Epidemiol. Infect. Dis., 2014, vol. 19, no. 6, pp. 16–20.

  6. 6

    Sergeeva, E.I., Ternovoi, V.A., Chausov, E.V., Berillo, S.A., Demina, O.K., Shikov, A.N., Plasunova, I.V., Kartashov, M.J., and Agafonov, A.P., Imported cases of dengue fever in Russia during 2010–2013, Asian Pac. J. Trop. Med., 2015, vol. 8, no. 2, pp. 90–93.

  7. 7

    Larichev, V.F., Saifullin, M.A., Akinshina, Yu.A., Khutoretskaya, N.V., and Butenko, A.M., Imported cases of arbovirus infections in the Russian Federation., Epidemiol. Infect. Dis., 2012, vol. 1, pp. 35–38.

  8. 8

    Bakhmet’eva, S.V., Pukhovskaya, N.M., Zdanovskaya, N.I., Ivanov, L.I., Belozerova, N.B., Utkina, O.M., Zhuravlev, Ya.A., and Larichev, V.F., Etiological decoding of imported cases of tropical fevers in the Far Eastern region, Dal’nevost. Zh. Infekts. Patol., 2014, vol. 25, no. 25, pp. 91–93.

  9. 9

    Keasey, S.L., Pugh, C.L., Jensen, S.M.R., Smith, J.L., Hontz, R.D., Durbin, A.P., Dudley, D.M., O’Connor, D.H., and Ulrich, R.G., Antibody responses to Zika virus infections in environments of flavivirus endemicity, Clin. Vaccine Immunol., 2017, vol. 24, no. 4, p. e00036-17.

  10. 10

    Ganushkina, L.A., Patraman, I.V., Rezza, G., Migliorini, L., Litvinov, S.K., and Sergiev, V.P., Detection of Aedes aegypti, Aedes albopictus, and Aedes koreicus in the area of Sochi, Russia, Vector Borne Zoonotic Dis., 2016, vol. 16, no. 1, pp. 58–60.

  11. 11

    Henchal, E.A. and Putnak, J.R., The dengue viruses, Clin. Microbiol. Rev., 1990, vol. 3, pp. 376–396.

  12. 12

    Luchinina, S.V., Semenov, A.I., Stepanova, O.N., Pogodina, V.V., Gerasimov, S.G., Shcherbinina, M.S., Kolesnikova, L.I., and Suslova, T.A., Vaccinal prevention of tick-borne encephalitis in Chelyabinsk region: Dynamics of vaccination, population immunity, analysis of TBE cases in vaccinated persons, Epidemiol. Vaccine Prev., 2016, vol. 15, no. 1 (86), pp. 67–76.

  13. 13

    State Report “On the State of Sanitary and Epidemiological Welfare of the Population in the Sverdlovsk Region in 2016”, Yekaterinburg, 2017.

  14. 14

    Gritsun, T.S., Lashkevich, V.A., Gould, E.A., Tick-borne encephalitis, Antiviral Res., 2003, vol. 57, pp. 129–146.

  15. 15

    Loktev, V.B., Taxonomy of flaviviruses and their genetic diversity, in Tick-Borne Infections in the Siberian Region, Vlasov, V.V. and Repin, V.E., Eds., Novosibirsk: Siberian Branch Russ. Acad. Sci., 2011.

  16. 16

    Leonova, G.N., Kondratov, I.G., Ternovoi, V.A., Romanova, E.V., Protopopova, E.V., Chausov, E.V., Pavlenko, E.V., Ryabchikova, E.I., Belikov, S.I., and Loktev, V.B., Characterization of Powassan viruses from Far Eastern Russia, Arch. Virol., 2009, vol. 154, no. 5, pp. 811–820.

  17. 17

    Kozlova, A.A., Butenko, A.M., Larichev, V.F., Azaryan, A.R., Grishanova, A.P., Ivashchenko, E.I., Shendo, G.L., Dzagurova, T.K., Pilikova, O.M., and Vasilenko, N.F., The study of the area of distribution of west Nile virus in the territory of the European part of Russia, the results of serological epidemiological researches, report 1: Astrakhan region, Krasnodar krai, Stavropol krai, Saratov region, Epidemiol. Infect. Dis., 2016, vol. 21, no. 5, pp. 244–252.

  18. 18

    Slon Campos, J.L., Poggianella, M., Marchese, S., Mossenta, M., Rana, J., Arnoldi, F., Bestagno, M., and Burrone, O.R., DNA-immunization with dengue virus E protein domains I/II, but not domain III, enhances Zika, West Nile and Yellow Fever virus infection, PLoS One, 2017, vol. 12, no. 7, p. e0181734.

  19. 19

    McAuley, A.J., Sawatsky, B., Ksiazek, T., Torres, M., Korva, M., Lotrič-Furlan, S., Avšič-Županc, T., von Messling, V., Holbrook, M.R., Freiberg, A.N., Beasley, D.W.C., and Bente, D.A., Cross-neutralization of viruses of the tick-borne encephalitis complex following tick-borne encephalitis vaccination and/or infection, NPJVaccines, 2017, vol. 2, p. 5.

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Correspondence to V. A. Ternovoi.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all participants involved in the study.


The authors declare that they have no conflict of interest.

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Translated by A. Ostyak

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Ternovoi, V.A., Plyasunova, I.V., Sementsova, A.O. et al. Identification of Markers for Dengue Infection in Patients after Visiting Dengue-Endemic Countries. Mol. Genet. Microbiol. Virol. 34, 188–194 (2019) doi:10.3103/S0891416819030066

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  • dengue virus
  • antibodies
  • enzyme immunoassay
  • tick-borne encephalitis virus