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Identification of Markers for Dengue Infection in Patients after Visiting Dengue-Endemic Countries

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Abstract

In studied blood serum samples from Russian patients hospitalized with suspected dengue fever, one or more markers of dengue fever have been detected, namely, viral RNA, NS1 antigen, and IgM or IgG antibodies. Moreover, only the combined use of various immunological and genetic methods provided a more effective diagnosis of dengue fever. The detection of IgG to dengue virus in patients with high titers of IgG to tick-borne encephalitis virus (TBEV) and, conversely, the detection of IgG to TBEV virus in patients with high titers of IgG to dengue has been documented. The results confirm the literature data on pronounced antigenic cross-sections between different representatives of flaviviruses, which may complicate the ELISA diagnosis of TBEV and dengue in patients. Genotyping of obtained dengue virus isolates showed that imported cases of dengue fever are caused by subtypes 1–4 of the dengue virus, with the prevalence of infection cases caused by dengue virus 1. Sequencing of these dengue virus isolates, including genome-wide, showed that viral isolates obtained on the territory of Russia are phylogenetically close to modern variants of the dengue virus circulating in the countries of Southeast Asia.

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Correspondence to V. A. Ternovoi.

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COMPLIANCE WITH ETHICAL STANDARDS

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all participants involved in the study.

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The authors declare that they have no conflict of interest.

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Translated by A. Ostyak

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Ternovoi, V.A., Plyasunova, I.V., Sementsova, A.O. et al. Identification of Markers for Dengue Infection in Patients after Visiting Dengue-Endemic Countries. Mol. Genet. Microbiol. Virol. 34, 188–194 (2019) doi:10.3103/S0891416819030066

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Keywords:

  • dengue virus
  • antibodies
  • enzyme immunoassay
  • tick-borne encephalitis virus