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The Effect of a Single Heating Treatment on Laboratory Mice Behavior, Adult Neurogenesis, and the Expression of Heatshock Protein HSP-70

  • O. V. Perepelkina
  • N. A. Ogienko
  • I. G. Lilp
  • D. G. Garbuz
  • A. V. Revishchin
  • G. V. Pavlova
  • I. I. PoletaevaEmail author
RESEARCH ARTICLE
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Abstract

Male hybrid mice (F1 CBA × C57BL/6J) were kept for 1.5 h at 39.5°С. In cognitive tests (extrapolation and puzzle-box elementary logic task solution), mice of the experimental groups (after exposure to an elevated temperature) solved the tasks more quickly and more successfully, while their activity in the “small open field” (in which no stress-inducing stimuli were present) and in the Porsolt test (unescapable swimming) was higher than in control mice. In separate experiments, the temporary impairment in the adult neurogenesis (the decrease of new cell numbers detected immunо-histochemically by the marker Ki67) after elevated temperature exposure was demonstrated in both the subgranular area of dentate fascia and in the subventricular proliferative zone of the forebrain. The heating treatment was accompanied by the increase of HSP-70 expression at the time point 3 h after the treatment, while no differences from control were found after 24 h.

Keywords:

hyperthermia behavioral activation cognitive ability elementary logic task adult neurogenesis heat shock proteins hybrid F1 mice. 

Notes

FUNDING

The work was supported by the Russian Foundation for Basic Researches (grant no. 17-29-01001 and state registration project AAAA-A16-116021660055-1.

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. Experiments were performed according to the Directive 2010/63/EU of European Parliament and European Union Council on welfare of animals used for scientific goals.

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Copyright information

© Allerton Press, Inc. 2019

Authors and Affiliations

  • O. V. Perepelkina
    • 1
  • N. A. Ogienko
    • 1
  • I. G. Lilp
    • 1
  • D. G. Garbuz
    • 2
  • A. V. Revishchin
    • 3
  • G. V. Pavlova
    • 3
  • I. I. Poletaeva
    • 1
    Email author
  1. 1.Department of Biology, Moscow State UniversityMoscowRussia
  2. 2.Institute of Molecular Biology, Russian Academy of SciencesMoscowRussia
  3. 3.Institute of Gene Biology, Russian Academy of SciencesMoscowRussia

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