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Comparative Analysis of Paddlefish (Polyodon spathula) Populations’ Genetic Structure with Regard to Microsatellite DNA Markers

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Abstract

Comparative characteristics in genetic structure of cultured Ukrainian and Polish populations and the natural paddlefish populations from the United States is reported for three microsatellite DNA markers: Psp21, Psp26, and Psp28. The average Na value was 6.1 and 5.5 for the Ukrainian and Polish populations, respectively. The Na value for the natural populations was almost two times higher at 11.1 alleles on average. The mean values of observed (Но) heterozygosity were shown to exceed those of expected heterozygosity (Не) for both Ukrainian (0.709 > 0.616) and Polish (0.809 > 0.699) populations. The mean Но and Не values for the natural populations were in the range of genetic equilibrium according to the Hardy–Weinberg equation at 0.817 and 0.813, respectively. According to the data presented, changes in the total number of allelic variants in the cultured populations as compared to natural populations were demonstrated. The heterozygosity level values obtained and negative values of the fixation index Fis for the cultured populations point at the absence of inbreeding at the present stage of paddlefish cultivation, and this is indicative of a sufficient number of breeders with a heterozygous genotype for reproduction under aquaculture conditions.

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Funding

This work was not supported by any specific grant from financial organizations in the state, commercial, or noncommercial sector.

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Correspondence to Kh. M. Kurta or O. O. Malysheva or V. G. Spyrydonov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by S. Semenova

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Kurta, K.M., Malysheva, O.O. & Spyrydonov, V.G. Comparative Analysis of Paddlefish (Polyodon spathula) Populations’ Genetic Structure with Regard to Microsatellite DNA Markers. Cytol. Genet. 54, 31–37 (2020). https://doi.org/10.3103/S0095452720010107

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Keywords:

  • Polyodon spathula
  • DNA markers
  • microsatellites
  • genetic structure
  • alleles
  • loci
  • polymorphism