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Cytology and Genetics

, Volume 52, Issue 6, pp 416–421 | Cite as

Molecular Organization of 5S Ribosomal DNА of Deschapmpsia antarctica

  • O. O. Ishchenko
  • І. І. Panchuk
  • І. O. Andreev
  • V. A. Kunakh
  • R. A. VolkovEmail author
Article
  • 12 Downloads

Abstract

Deschampsіa antarctіca, one of the two angiosperm species growing in the extreme conditions of Antarctica, is a unique model for studying the relationship between genetic polymorphism and such factors of evolution as isolation, migration and adaptation to new environmental conditions. Molecular markers represent a useful tool for the investigation of these questions. To examine the potential of 5S rDNA to be used for the discrimination of D. antarctica populations we cloned and sequenced this genomic region for plants from three populations of this species from Maritime Antarctica. It was shown that in the genome of D. antarctica at least two structural classes of 5S rDNA are present, which differ by numerous base substitutions and insertions/deletions in the intergenic spacer. Based on this structural polymorphism we propose to apply this region for the evaluation of the intraspecific genetic diversity of D. antarctica.

Keywords:

genetic polymorphism molecular evolution 5S rDNA Deschampsіa antarctіca 

Notes

ACKNOWLEDGMENTS

Authors express their gratitude to the National Antarctic Scientific Center of Ukraine and personally to Dr. I.A. Kozeretska (Taras Shevchenko Kyiv National University) for the material provided for research.

COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.

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Copyright information

© Allerton Press, Inc. 2018

Authors and Affiliations

  • O. O. Ishchenko
    • 1
  • І. І. Panchuk
    • 1
  • І. O. Andreev
    • 2
  • V. A. Kunakh
    • 2
  • R. A. Volkov
    • 1
    Email author
  1. 1.Yuriy Fedkovych Chernivtsi National UniversityChernivtsiUkraine
  2. 2.Institute of Molecular Biology and Genetics, National Academy of Sciences of UkraineKyivUkraine

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