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Moscow University Chemistry Bulletin

, Volume 74, Issue 3, pp 146–148 | Cite as

Quantification of Hexamethylenediamide Bis(N-Monosuccinyl-L-Glutamyl-L-Lysine) in Blood Plasma by HPLC–MS

  • A. A. Litvin
  • G. K. Kolyvanov
  • E. V. BlynskayaEmail author
  • O. G. Grybakina
  • P. O. Bochkov
  • R. V. Shevchenko
  • V. P. Zherdev
Article
  • 2 Downloads

Abstract

The quantitative determination of a new compound hexamethylenediamide bis(N-monosuccinyl-L-glutamyl-L-lysine) (GK-2) in rat blood plasma is developed and validated by the HPLC–MS technique. The method is linear in the range of 10 to 1000 ng/mL. It is shown that 81.62% of GK-2 is recovered from the plasma. The lower limit of its quantification was 10 ng/mL.

Keywords:

hexamethylenediamide bis(N-monosuccinyl-L-glutamyl-L-lysine) blood plasma HPLC–MS quantification Alzheimer’s disease 

Notes

FUNDING

This work was supported by the Ministry of Education and Science of the Russian Federation (state contract no. 14.N08.12.0051).

REFERENCES

  1. 1.
    Obianyo, O., Biochim. Biophys. Acta, 2013, vol. 1834, no. 10, p. 2213.CrossRefGoogle Scholar
  2. 2.
    Seredenin, S.B. and Gudasheva, T.A., RF Patent 2410392, 2010.Google Scholar
  3. 3.
    Gudasheva, T.A., Povarnina, P.Yu., Antipova, T.A., and Seredenin, S.B., Neurosci. Med., 2014, vol. 5, no. 2, p. 101.CrossRefGoogle Scholar
  4. 4.
    Validierung analytischer Verfahren der fictiven Firma “Muster” fur die Arzneimittel-Herstellung (der Bundesverband der Arzneimittel-Hersteller (BAH)), 2004.Google Scholar

Copyright information

© Allerton Press, Inc. 2019

Authors and Affiliations

  • A. A. Litvin
    • 1
  • G. K. Kolyvanov
    • 1
  • E. V. Blynskaya
    • 1
    Email author
  • O. G. Grybakina
    • 1
  • P. O. Bochkov
    • 1
  • R. V. Shevchenko
    • 1
  • V. P. Zherdev
    • 1
  1. 1.Zakusov Research Institute of PharmacologyMoscowRussia

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