GbABR1 is associated with Verticillium wilt resistance in cotton
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We cloned the GbABR1 gene from highly resistant Gossypium barbadense Xinhai15 based on the candidate genes screened by transcriptome sequencing that were related to resistance to Verticillium wilt. A sequence characteristic analysis showed that GbABR1 was an ERF subfamily B4 member and was a new member of the AP2 family of sea-island cotton. The GbABR1 gene was expressed highly in roots compared with the levels in leaves and stems in cotton. Expression was enhanced significantly in cotton after infection by Verticillium dahliae, indicating that GbABR1 probably plays an important role in the response to biotic stress. The results of subcellular localisation showed that GFP:GbABR1 was localised to the nucleus. GbABR1 silencing via the virus-induced gene silencing (VIGS) method indicated that the incidence of disease and the disease index in VIGS-silenced plants were much higher than in the control after infection by Verticillium dahliae. The GbABR1-overexpressing Arabidopsis plants showed similar resistance to Verticillium dahliae compared to the wild type. These results indicate that the GbABR1 gene plays a positive role in resistance to Verticillium wilt. The GbABR1-overexpressing Arabidopsis plants presented dwarfism, early maturation and early bolting compared with wild-type plants, suggesting that GbABR1 also participates in growth and development.
KeywordsGossypium barbadense GbABR1 Transcription factor Verticillium wilt Function
This work was supported by the National Key Research and Development Program of China (NO:2016YFD0101900), the Natural Science Foundation of China (NSFC grant No. 31571724, U1704104), Innovation and Talent Introduction Program on Crop Stress Biology (111 Project), Henan outstanding talent project (154200510006).
- Abeles FB, Morgan PW, Saltveit ME Jr (1992) Ethylene in plant biology, vol 63, 2nd edn. Academic Press, New York, pp 3899–3911Google Scholar
- Berrocal-Lobo, M., Molina, A., and Solano, R. (2002). Constitutive expression of ETHYLENE RESPON SEFACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi. Plant J 29:23–32. Doi: https://doi.org/10.1046/j.1365-313x.2002.01191.x
- Broekaert WF, Delaure SL, De Bolle MF, Cammue BP (2006) The role of ethylene in host–pathogen interactions. Annu Rev Phytopathol 44:393–416. https://doi.org/10.1146/annurev.phyto.44.070505.143440 CrossRefPubMedGoogle Scholar
- Chia, KF (2013) The Arabidopsis transcription factor ERF13 negatively regulates defense against Pseudomonas syringae. Dissertations & Theses, University of California, San Diego.Google Scholar
- Dietz KJ, Vogel MO, Viehhauser A (2010) AP2/EREBP transcription factors are part of gene regulatory networks and integrate metabolic, hormonal and environmental signals in stress acclimation and retrograde signaling. Protoplasma 245:3–14. https://doi.org/10.1007/s00709-010-0142-8 CrossRefPubMedGoogle Scholar
- Feng JX, Liu D, Pan Y, Gong W, Ma LG, Luo JC, Deng XW, Zhu YX (2005) An annotation update via cDNA sequence analysis and comprehensive profiling of developmental, hormonal or environmental responsiveness of the Arabidopsis AP2/EREBP transcription factor gene family. Plant Mol Biol 59:853–868. https://doi.org/10.1007/s11103-005-1511-0 CrossRefPubMedGoogle Scholar
- Gayoso C, Pomar F, Novo-Uzal E, Merino F, deIlarduya OM (2010) The Ve-mediated resistance response of the tomato to V. dahliae involves H2O2, peroxidase and lignins and drives PAL gene expression. BMC Plant Biol 10:232. https://doi.org/10.1186/1471-2229-10-232 CrossRefPubMedPubMedCentralGoogle Scholar
- Lee JH, Yoon HJ, Terzaghi W, Martinez C, Dai M, Li J, Byun MO, Deng XW (2010) DWA1 and DWA2, two Arabidopsis DWD protein components of CUL4-based E3 ligases, act together as negative regulators in ABA signal transduction. Plant Cell 22:1716–1732. https://doi.org/10.1105/tpc.109.073783 CrossRefPubMedPubMedCentralGoogle Scholar
- Meng Xianpeng (2009). Isolation and characterization of transcription factor ERF related to disease-resistance in Gossypium barbadense L. Chinese Academy of Agricultural Sciences, Dissertations & Theses.Google Scholar
- Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002) DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration and cold-inducible gene expression. Biochem Biophys. Biochem Biophys Res Commun 12:998–1009. https://doi.org/10.1006/bbrc.2001.6299. CrossRefGoogle Scholar
- Scarpeci TE, Frea VS, Zanor MI, Valle EM (2016) Overexpression of AtERF019delays plant growth and senescence and improves drought tolerance in Arabidopsis. J Exp Bot. https://doi.org/10.1093/jxb/erw429
- Xu Z S, Xia L Q, Chen M, et al. (2007). Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 (TaERF1) that increases multiple stress tolerance. https://doi.org/10.1007/s11103-007-9237-9.
- Zhang Q., Gao M., Wu L., Wang Y., Chen Y. (2016a). Divergent expression patterns in two Vernicia species revealed the potential role of the hub gene VmAP2/ERF036 in resistance to fusarium oxysporum in Vernicia montana. Genes, Dec 1;7(12). https://doi.org/10.3390/genes7120109