, Volume 73, Issue 2, pp 137–144 | Cite as

Effect of sodium selenate on photosynthetic efficiency, antioxidative defence system and micronutrients in maize (Zea mays)

  • Sucheta SharmaEmail author
  • Anju Sharma
  • Dhanwinder Singh
Original Article


Oxidative stress or formation of faulty proteins due to non-specific replacement of sulphur by selenium(Se)/mineral imbalance can be one of the reasons for Se phytotoxicity. Present investigation reports the effect of Se on photosynthetic efficiency, anti-oxidative status and micronutrients in maize. Selenate-Se application (1–32 mg kg−1 soil) showed significant growth reduction after 30 days of sowing and all the plants died with concentration higher than 4 mg kg−1 soil. Lower Se doses increased dry matter, chlorophyll, proline and activities of defence enzymes viz. peroxidase, catalase and superoxide dismutase and decreased malondialdehyde, glutathione and glutathione reductase activity as compared to control. All the parameters showed the reverse trend with Se treatment of 4 mg kg−1 soil. Concentration of nutrients (K, P, S, Mn, Mg and Ca) in leaves decreased with application of increasing Se doses. Shoot and root weight decreased (8.5–31.9% and 12–24%, respectively) in response to varying Se doses and highest Se accumulation in these tissues was observed with Se @ 4 mg kg−1 soil. The phyto-toxic effects of higher Se doses may be due to its prooxidant effects and disturbances in nutrients level.


Biochemical composition Growth Selenium Maize 



The authors are thankful to CSIR, New Delhi (38(1362/13/EMR-II) for financial support and research fellowship to AS.


  1. Aebi H (1983) Catalase. In: Bergmeyer HO (ed) Methods of enzymatic analysis, vol 111. Academic Press, New York, 273ppGoogle Scholar
  2. Akbulut M, Cakir S (2010) The effects of selenium phytotoxicity on the antioxidant systems of leaf tissues in barley (Hordeum vulgare L.) seedlings. Plant Physiol Biochem 48:160–166CrossRefPubMedGoogle Scholar
  3. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  4. Chen Y, Mo H-Z, Zheng M-Y, Xian M, Qi Z-Q, Li YQ, Hu LB, Chen J, Yang LF (2014) Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa. PLoS One 9(10):e110904CrossRefPubMedPubMedCentralGoogle Scholar
  5. Combs GF Jr (2015) Biomarkers of selenium status. Nutrients 7:2209–2236CrossRefPubMedPubMedCentralGoogle Scholar
  6. Dhillon KS, Dhillon SK (2002) Selenium adsorption in soils as influenced by different anions. J Pl Nutr Soil Sci 163:577–582CrossRefGoogle Scholar
  7. Fargasova A, Pastierova J, Svetkova K (2006) Effect of Se metal pair combinations (Cd, Zn, Cu, Pb) on photosynthetic pigments production and metal accumulation in Sinapis alba seedling. Plant Soil Environ 52:8–15CrossRefGoogle Scholar
  8. Feng R, Wei C, Tu S (2013) The roles of selenium in protecting plants against abiotic stresses. Environ Exp Bot 87:58–68CrossRefGoogle Scholar
  9. Filek M, Zembela M, Kornas A, Walas S, Mrovic H, Hartikienen H (2010) The uptake and translocation of macro- and microelements in rape and wheat seedlings as affected by selenium supply level. Plant Soil 336:303–312CrossRefGoogle Scholar
  10. Fridovich I (1985) Superoxide dismutases. Annu Rev Biochem 64:97–112CrossRefGoogle Scholar
  11. Garcia-Banuelos ML, Hermosillo-Cereceres MA, Sánchez E (2011) The importance of selenium biofortification in food crops. Curr Nutr Food Sci 7:181–190CrossRefGoogle Scholar
  12. Garousi F, Veres S, Bódi É, Várallyay S, Kovács B (2015) Role of selenite and selenate uptake by maize plants in chlorophyll A and B content. Int J Biol Biomolec Agric Food Biotechnol Engineer 9:625–668Google Scholar
  13. Garousi F, Veres S, Bódi É, Várallyay S, Kovács B (2016) Assessment and comparison of selenium-enriched maize with sodium selenite and sodium selenate. Agrártudományi Közlemények 68:11–15Google Scholar
  14. Gomes-Junior RA, Gratao PL, Gaziola SA, Mazzafera P, Lea PJ, Azeredo RA (2007) Selenium-induced oxidative stress in coffee cell suspension cultures. Funct Plant Biol 34:449–456CrossRefGoogle Scholar
  15. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139PubMedGoogle Scholar
  16. Hawrylak-Nowak B (2008) Effect of selenium on selected macronutrients in maize plants. J Elem 13:513–519Google Scholar
  17. Hawrylak-Nowak B, Matraszek R, Magdalena P (2015) The dual effects of two inorganic selenium forms on the growth, selected physiological parameters and macronutrients accumulation in cucumber plants. Acta Physiol Plant 37:41–52CrossRefGoogle Scholar
  18. Heath RL, Packer L (1968) Photo-peroxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198CrossRefPubMedGoogle Scholar
  19. Jezek P, Hlusek J, Losak T, Juzl M, Elzner P, Kracmar S, Bunka F, Martensson A (2012) Effect of foliar application of selenium on the content of selected amino acids in potato tubers (Solanum tuberosum L.) Plant Soil Environ 57(7):315–320CrossRefGoogle Scholar
  20. Keles Y, Oncel I (2002) Response of antioxidative defence system to temperature and water stress combinations in wheat seedlings. Plant Sci 163:783–790CrossRefGoogle Scholar
  21. Labanowska M, Filek M, Koscielniak J, Kurdziel M, Kulis E, Hartikainen H (2012) The effects of short-term selenium stress on Polish and Finnish wheat seedlings—EPR, enzymatic and fluorescence studies. J Plant Physiol 69:275–284CrossRefGoogle Scholar
  22. Lehotai N, Kolbert Z, Peto A, Feigl G, Ördög A, Kumar D, Tari L, Erdei L (2012) Selenite-induced hormonal and signaling mechanisms during root growth of Arabidopsis thaliana L. J Exp Bot 63:5677–5687CrossRefPubMedGoogle Scholar
  23. Longchamp M, Castrec-Rouelle M, Biron P, Bariac T (2015) Variations in the accumulation, localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenate. Food Chem 182:128–135CrossRefPubMedGoogle Scholar
  24. Lyons GH, Stangoulis JCR, Graham RD (2005) Tolerance of wheat (Triticum aestivum) to high soil and solution selenium levels. Plant Soil 270:179–188CrossRefGoogle Scholar
  25. Mallick N, Mohn FH (2003) Use of chlorophyll fluorescence in metal-stress research: a case study with green microalga Scenedesmus. Ecotoxicol Environ Saf 55:64–69CrossRefPubMedGoogle Scholar
  26. Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the auto-oxidation of pyragallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:169–174CrossRefGoogle Scholar
  27. Mroczek-Zdyrska M, Wójcik M (2012) The influence of selenium on root growth and oxidative stress induced by lead in Vicia faba L. minor plants. Biol Trace Elem Res 147:320–328CrossRefPubMedGoogle Scholar
  28. Nowak J, Kaklewski K, Ligocli M (2004) Influence of selenium on oxido-reductive enzymes activity in soil and in plants. Soil Biol Biochem 36:1553–1558CrossRefGoogle Scholar
  29. Pazurkiewicz-Kocot K, Galas W, Kita A (2003) The effect of selenium on the accumulation of some metals in Zea mays L. plants treated with indole-acetic acid. Cell Mol. Biol Lett 8:97–103Google Scholar
  30. Pilon-Smits EAH, Quinn CF (2010) Selenium metabolism in plants. Cell Biol Metals Nutr 17:225–241CrossRefGoogle Scholar
  31. Ramos SJ, Faquin V, de Almeida HJ, A´vila FW, Guimaraães Guilherme LR, Alves Bastos CE, A´vila PA (2011) Selenate and selenite on yield, mineral nutrition and biofortification with selenium in lettuce cultivars. Rev Bras Cienc Solo 35:1347–1355CrossRefGoogle Scholar
  32. Saffaryazdi A, Lahouti M, Ganjeali A, Byat H (2012) Impact of selenium supplementation on growth and accumulation on spinach (Spinacia oleracea L.) plants. Not Sci Biol 4:95–100Google Scholar
  33. Shannon LM, Kay E, Lew JW (1966) Peroxidase isoenzymes from horseradish roots. J Biol Chem 241:2166–2172PubMedGoogle Scholar
  34. Sharma S, Bansal A, Dhillon KS, Dhillon SK (2010) Comparative effects of selenate and selenite on growth and biochemical composition of rapeseed (Brassica napus L.) Plant Soil 329:339–348CrossRefGoogle Scholar
  35. Sharma S, Gupta R, Singh D (2016) Variation in Se tolerance, accumulation, and growth parameters of different wheat cultivars. Commun Soil Sci Plant Anal 47:203–212CrossRefGoogle Scholar
  36. Ullah I, Ali M, Farooqi A (2010) Chemical and nutritional varieties of some maize varieties grown in NWFP. Pakistan Pak J Nutr 9:1113–1117CrossRefGoogle Scholar
  37. Van Hoewyk D (2013) A tale of two toxicities: malformed selenoproteins and oxidative stress both contribute to selenium stress in plants. Ann Bot 163:1–8CrossRefGoogle Scholar
  38. Witham FH, Blaydes DF, Devlin RM (1971) Experiments in plant physiology. Van Nostrand, New York, 245ppGoogle Scholar
  39. Yoshimoto N, Takahashi H, Smith FW, Yamaya T, Saito K (2002) Two distinct high affinity sulfate transporters with different inducibilities mediate uptake of sulfate in Arabidopsis roots. Plant J 29:465–473CrossRefPubMedGoogle Scholar

Copyright information

© Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2018

Authors and Affiliations

  1. 1.Department of BiochemistryPunjab Agricultural UniversityLudhianaIndia
  2. 2.Department of Soil SciencePunjab Agricultural UniversityLudhianaIndia

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