Oxidative stress response to aluminum oxide (Al2O3) nanoparticles in Triticum aestivum
- 137 Downloads
The development of nanotechnologies has increased the amount of manufactured metal oxide nanoparticles in the environment. In the view of nanoparticle dispersion to the environment, assessment of their toxicity becomes very crucial. Aluminum oxide (Al2O3) nanoparticles have wide range of use in industry as well as personal care products. The aim of this study was to evaluate the dose dependent effects of 13-nm-sized Al2O3 nanoparticles on wheat correlating with the appearance of enzymatic and non-enzymatic antioxidant defense response. Wheat roots were exposed to different concentrations of Al2O3 nanoparticles (5, 25 and 50 mg mL−1) for 96 h. The effects of Al2O3 nanoparticles were studied using different parameters such as H2O2 content, superoxide dismutase and catalase activity, lipid peroxidation, total proline, photosynthetic pigment and anthocyanin content. The results indicated that while Al2O3 nanoparticles caused a dose dependent increase in H2O2 content, superoxide dismutase activity, lipid peroxidation and proline contents, the catalase activity was decreased in compare the control. Moreover, total chlorophyll, chlorophyll a, carotenoids and anthocyanin contents reduced in the highest concentration 50 mg mL−1. In conclusion, Al2O3 nanoparticles caused oxidative stress in wheat after 96 h.
KeywordsTriticum aestivum Aluminum oxide nanoparticles Enzymatic antioxidants Non-enzymatic antioxidants Oxidative stress Photosynthetic pigments
This study is supported by Research Foundation of Marmara University (BAPKO) project number FEN-A-110915-0431.
- Cho YW, Park EH, Lim CJ (2000) Glutathione S-transferase activities of S-type and L-type thiol transferase from Arabidopsis thaliana. J Biochem Mol Biol 33:179–183Google Scholar
- Montillet JL, Chamnongpol S, Rustérucci C, Dat J, van de Cotte B, Agnel JP, Battesti C, Inzé D, Van Breusegem F, Triantaphylides C (2005) Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves. Plant Physiol 138:1516–1526CrossRefPubMedPubMedCentralGoogle Scholar
- Riahi-Madvar A, Rezaee F, Jalali V (2012) Effects of alumina nanoparticles on morphological properties and antioxidant system of Triticum aestivum. Iran J Plant Physiol 3:595–603Google Scholar
- Shirazi AM, Shariati F, Keshavarz AK, Ramezanpour Z (2015) Toxic effect of aluminum oxide nanoparticles on green micro–algae Dunaliella salina. Int J Environ Res 9:585–594Google Scholar