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Hypocrealean fungi associated with populations of Ips typographus in West Carpathians and selection of local Beauveria strains for effective bark beetle control

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Abstract

In Slovakia, a diversity of entomopathogenic fungi (Ascomycota, Hypocreales) associated with outbreaks of Ips typographus was studied in 81 localities and as many as 113 in vitro cultures of five entomopathogenic species were isolated from infected individuals: Beauveria bassiana (87 isolates), B. pseudobassiana (14 isolates), B. caledonica (6 isolates), Lecanicillium lecanii (4 isolates) and Isaria farinosa (2 isolates). B. pseudobassiana is recorded in natural populations of I. typographus for the first time. Biological properties of selected Beauveria isolates, including colony growth, biomass production, conidia yield and pathogenicity to I. typographus adults, were studied in a series of laboratory bioassays and much intra- and interspecific variability was detected. B. bassiana isolates produced biomass or conidia at significantly higher rate than B. pseudobassiana and B. caledonica isolates. Two B. bassiana isolates were selected as the most virulent to bark beetle adults, demonstrating a mean LC50 ranging from 0.72 to 2.05 × 106 conidia ml−1, and were qualified as promising candidates for biocontrol of I. typographus. Their virulence was significantly higher than that of the mycoinsecticides Boverol®, which was used as a reference strain in the virulence bioassays.

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Acknowledgement

This research was funded by the Operational Program of Research and Development and co-financed with the European Fund for Regional Development (EFRD). Grant: ITMS 26220220087: The development of ecological methods to control chosen forest pests in vulnerable mountainous regions of Slovakia.

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Authors and Affiliations

  1. Institute of Forest Ecology, Slovak Academy of Sciences, Akademická 2, 949 01, Nitra, Slovakia

    Marek Barta

  2. Natural History Museum Bratislava, Vajanského nábrežie 2, 810 06, Bratislava, Slovakia

    Ivona Kautmanová

  3. Institute of Zoology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 06, Bratislava, Slovakia

    Helena Čičková

  4. State Forests of TANAP, 059 60, Tatranská Lomnica, Slovakia

    Ján Ferenčík

  5. Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 06, Bratislava, Slovakia

    Štefan Florián

  6. Central European University in Skalica, Kráľovská 11, 909 01, Skalica, Slovakia

    Július Novotný

  7. Scientica s.r.o, Hybešova 33, 831 06, Bratislava, Slovakia

    Milan Kozánek

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Barta, M., Kautmanová, I., Čičková, H. et al. Hypocrealean fungi associated with populations of Ips typographus in West Carpathians and selection of local Beauveria strains for effective bark beetle control. Biologia 73, 53–65 (2018). https://doi.org/10.2478/s11756-018-0005-x

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