Abstract
Some species of Psychotria (Rubiaceae) are important in herbal medicine, where their extracts are used internally for infections of the female reproductive system, bronchitis, gastrointestinal disturbances, skin irritations, tumors, ulcers, and eye disturbances. The antiproliferative and genotoxic effects of Psychotria myriantha Mull. Arg. and P. leiocarpa Cham. et Schlecht infusions on the Allium cepa L. cell cycle were evaluated. The teas were prepared by infusing the leaves in distilled water, in two concentrations: 1.13 mg/mL and 6.78 mg/mL. Three groups of four bulbs were used for each Psychotria species. After the bulbs rooted in distilled water, they were transferred to the teas for 24 hours, except for the control that stayed in water. The rootlets were then collected, fixed in ethanol-acetic acid (3:1) for 24 hours, and stored in 70% ethanol. For each group of bulbs, 4000 cells were analyzed, calculating the mitotic indexes, submitting them to statistic analysis, using the χ 2 test (p = 0.05). The results showed a decrease in mitotic index with an increase in tea concentration in both species. In P. leiocarpa, the mitotic index values differed significantly between the control and concentration of 6.78 mg/mL (χ 2 = 9.863). For P. myriantha, the values referring to the mitotic index differed greatly between the control and the treatments (χ 2 = 124.8). With this study, it was determined that P. myriantha and P. leiocarpa infusions possess antiproliferative effects on the A. cepa cell cycle, and teas of P. myriantha also have genotoxic activity.
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Abbreviations
- CCI:
-
cell cycle irregularities
- MI:
-
mitotic index
References
Adjibadé Y. 1989. Pharmacognosie du Psychotria forsteriana A. Gray (Rubiaceae) — aspects botanique, chimique et essais pharmacologiques preliminaries. PhD Thesis, Université Louis Pasteur de Strasbourg I.
Almassy Jr A.A., Lopes R.C, Armond C., Silva F. & Casali V.W.D. 2005. Folhas de Chá: Plantas Medicinais na Terapęutica Humana. UFV, Viçosa, 233 pp., ISBN: 85-7269-214-2.
Andersson L.A. 1992. A provisional checklist of neotropical Rubiaceae. Scripta Botanica Belgica 1: 1–119.
Ayres M., Ayres Jr M., Ayres D.L. & Santos A.S. 2003. BioEstat 3.0: Aplicaçőes estatísticas nas área de Cięncias Biológicas e Médicas. Sociedade Civil Mamirauá, Belém, MCT-CNPq, Conservation International, 290 pp.
Burim R.V., Canalle R., Lopes J.L.C. & Takahashi C.S. 1999. Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo. Genet. Mol. Biol. 22: 401–406.
Cabrera G.L. & Rodríguez D.M.G. 1999. Genotoxicity of soil from farmland irrigated with wastewater using three plant bioassays. Mutat. Res. 426: 211–214.
Camparoto M.L., Teixeira R.O., Mantovani M.S. & Vicentini V.E.P. 2002. Effects of Maytenus ilicifolia Mart. and Bauhinia candicans Benth infusions on onion root-tip and rat bone-marrow cells. Genet. Mol. Biol. 25: 85–89.
Çelik T.A. & Aslantürk Ö.S. 2006. Anti-mitotic and anti-genotoxic effects of Plantago lanceolata aqueous extract on Allium cepa root tip meristem cells. Biologia 61: 693–697.
Çelik T.A. & Aslantürk Ö.S. 2007. Cytotoxic and genotoxic effects of Lavandula stoechas aqueous extracts. Biologia 62: 292–296.
Dias F.L. & Takahashi C.S. 1994. Cytogenetic evaluation of the effect of aqueous extracts of the medicinal plants Alpinia nutans Rose (Zingiberaceae) and Pogostemum heyneanus Benth (Labiateae) on wistar rats and Allium cepa Linn. (liliaceae) root tip cells. Braz. J. Genet. 17: 175–180.
Dillenburg C.R. & Porto M.L. 1985. Tribo Psychotrieae. Boletim Instituto de Biocięncias da UFRGS 39: 1–76.
Elisabetsky E., Amador T.A. & Leal M.B. 1997. Merging ethnopharmacology with chemotaxonomy: an approach to unveil bioactive natural products. The case of Psychotria alkaloids as potencial analgesics. Cięncia e Cultura 49: 378–385.
Fachinetto J.M., Bagatini M.D., Durigon J., Silva A.C.F. & Tedesco S.B. 2007. Anti-proliferative effect of infusion of Achyrocline satureoides on the Allium cepa cell cycle. Braz. J. Pharmacognosy 17: 49–54.
Fiskesjö G. 1994. Allium Test II: Assessment of chemical’s genotoxic potencial by recording aberrations in chromosomes and cell divisions in root tips of Allium cepa L. Environ. Toxicol. Wat. Qual. 9: 235–241.
Gadano A., Gurni A., López P., Ferraro G. & Carballo M.L. 2002. In vitro genotoxic evaluation of the medicinal plant Chenopodium ambrosioides. J. Ethnopharmacol. 81: 11–16.
Grisolia C.K., Takahashi C.S. & Ferrari I. 1995. In vitro and in vivo tests in humans confirm that the antimalarial drug mefloquine is not mutagenic. Braz. J. Genet. 18: 611–615.
Guerra M. & Souza M.J. 2002. Como observar cromossomos: um guia de técnicas em citogenética vegetal, animal e humana. FUNPEC, Pernambuco, 131 pp.
Henriques A.T., Kerber V.A. & Moreno P.R.H. 2001. Alcalóides: generalidades e aspectos básicos, pp. 651–666. In: Simőes C.M., Schenkel E.P., Gosmann G., Mello J.C.P., Mentz L.A. & Petrovick P.R. (eds) Farmacognosia: da Planta ao Medicamento, UFGRS, Porto Alegre.
Itoyama M.M., Picudo H.E.M.C. & Cordeiro J.A. 1997. Effects of caffeine on mitotic index in Drosophila prosaltans (Diptera). Braz. J. Genet. 20: 655–657.
Knoll M.F., Silva A.C.F., Canto-Dorow T.S. & Tedesco S.B. 2006. Effects of Pterocaulon polystachyum DC. (Asteraceae) on onion (Allium cepa) root-tip cells. Genet. Mol. Biol. 29: 539–542.
Lajis N.H., Mahmud Z. & Toia R.F. 1993. The alkaloids of Psychotria rostrata. Planta Med. 59: 383–384.
Leal M.B. 1994. Estudo psicofarmacológico de espécies de Psychotria (Rubiaceae) do estado do Rio Grande do Sul. MSc. Thesis, Universidade Federal do Rio Grande do Sul.
Perry L.M. 1980. Medicinal Plants of East and Southeast Asia: Rubiaceae. The MIT Press, Cambridge, 620 pp.
Ragunathan I. & Panneerselvam N. 2007. Antimutagenic potential of curcumin on chromosomal aberrations in Allium cepa. Journal of Zhejiang University 8: 470–475.
Roberts M.F. & Strack D. 1999. Biochemistry and physiology of alkaloids and betalains, pp. 17–78. In: Wink M. (ed.) Biochemistry of Plant Secondary Metabolism.
Santos L.V.D., Fett-Neto A.G., Kerber V.A., Elisabetsky E., Quirion J.C. & Henriques A.T. 2001. Indole monoterpene alkaloids from leaves of Psychotria suterella Mull. Arg. (Rubiaceae). Biochem. Syst. Ecol. 29: 1185–1187.
Silva C.R., Monteiro M.R., Araújo A.C. & Bezerra R.J.A.C. 2004. Absence of mutagenic and cytotoxic potentiality of senna (Cassia angustifolia Vahl.) evaluated by microbiological tests. Braz. J. Pharmacognosy 14: 1–3.
Smith L.B. & Downs R.J. 1956. Resumo preliminar das Rubiaceae de Santa Catarina. Sellowia 7: 13–86.
Teixeira R.O., Camparoto M.L., Mantovani M.S. & Vicentini V.E.P. 2003. Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L. in in vivo assays. Genet. Mol. Biol. 26: 551–555.
Vicentini V.E.P., Camparoto M.L., Teixeira R.O. & Mantovani M.S. 2001. Averrhoa carambola L., Syzygium cumini (L.). Skeels and Cissus sicyoides L.: medicinal herbal tea effects on vegetal and test systems. Acta Scient. 23: 593–598.
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Lubini, G., Fachinetto, J.M., Laughinghouse, H.D. et al. Extracts affecting mitotic division in root-tip meristematic cells. Biologia 63, 647–651 (2008). https://doi.org/10.2478/s11756-008-0108-x
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DOI: https://doi.org/10.2478/s11756-008-0108-x