Abstract
Based on the cDNA sequence encoding taxane 2α-O-benzoyltransferase (TBT) from Taxus yunnanensis (Gen-Bank Accession No.: AY970522), genomic sequences of TBTs from T. yunnanensis (TyTBT) and T. cuspidata (TcuTBT) were cloned for the first time. They both contain only one intron. The finding that the introns of TyTBT and TcuTBT are more diverse than their exons implies that the gene diversity is more within introns than within exons, which may be important for keeping the functions of the genes.
References
Baloglu, E. & Kingston, D.G.I. 1999. The taxane diterpenoids. J. Nat. Prod. 62: 1448–1472.
Croom, E.M.J. 1995. Taxus for toxol and toxids, pp. 37–70. In: Suffness, M. (ed.) Taxol Science and Applications, CRC Press, Boca Raton.
Furmanowa, M. & Syklowska-Baranek, K. 2000. Hairy root cultures of Taxus x media var. Hicksii Rehd. as a new source of paclitaxel and 10-deacetylbaccatin III. Biotechnol. Lett. 22: 683–686.
Han, K.H., Fleming, P., Walker, K., Loper, M., Chilton, W.S., Mocek, U., Gordon, M.P. & Floss, H.G. 1994. Genetic transformation of mature Taxus: an approach to genetically control the in vitro production of the anticancer drug, Taxol. Plant Sci. 95: 187–196.
Han, R.L., Hao, G. & Zhang, D.X. 2004. Interfamilial relationships of Santalales as revealed by chloroplast trnL intron sequences. J. Tropical Subtropical Bot. 12: 393–398.
Holton, R.A., Somoza, C., Kim, H.B., Liang, F., Biediger, R.J., Boatman, P.D., Shindo, M., Smith, C.C. & Kim, S. 1995. The total synthesis of paclitaxel starting with camphor. ACS Symp. Ser. 583: 288–301.
Jennewein, S. & Croteau, R. 2001. Taxol: biosynthesis, molecular genetics, and biotechnological applications. Appl. Microbiol. Biotechnol. 57: 13–19.
Kai, G., Miao, Z., Qiu, C., Zhang, L., Zhao, L., Li, Z., Xu, T., Zhang, L., Gong, Y., Zhao, D., Liu, D., Sun, X. & Tang, K. 2004. Molecular cloning and characterization of a taxadienol acetyl transferase cDNA from Taxus × media. Plant Sci. 167: 759–764.
Kohler, J. & Goldspiel, B.R. 1994. Evaluation of new drug Paclitaxel (Taxol). Pharmacotherapy 14: 3–34.
Shin, S.W., Kim, Y.S. & Lim, S. 2000. Elicitors for the regulation of baccatin III biosynthesis in plant cell culture system. Yakhak Hoechi 44: 60–65.
Walker, K. & Croteau, R. 2000. Taxol biosynthesis — molecular cloning of a benzoyl-CoA-taxane 2α-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli. Proc. Natl. Acad. Sci. USA 97: 13591–13596.
Walker, K. & Croteau, R. 2001. Molecules of interest: taxol biosynthetic genes. Phytochemistry 58: 1–7.
Yuan, Y.J., Wei, Z.J., Miao, Z.Q. & Wu, J.C. 2002. Acting paths of elicitors on taxol biosynthesis pathway and their synergistic effect. Biochem. Eng. J. 10: 77–83.
Yukimune, Y., Hara, Y., Nomura, E., Seto, H. & Yoshida, S. 2000. The configuration of methyl jasmonate affects paclitaxel and baccatin III production in Taxus cells. Phytochemistry 54: 13–17.
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Zhao, D., Li, L., Wang, Q. et al. Cloning of taxane 2α-O-benzoyltransferase (TBT) genomic DNA from Taxus . Biologia 61, 327–329 (2006). https://doi.org/10.2478/s11756-006-0059-z
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DOI: https://doi.org/10.2478/s11756-006-0059-z