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Dye decolorisation by laccase immobilised in lens-shaped poly(vinyl alcohol) hydrogel capsules

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Abstract

In this study, laccase (from Trametes versicolor, 8.3 U mg −1enz ) was used for the decolorisation of Saturn Blue L4G (10 mg L−1). The efficiency of the decolorisation (ratio between the amount of decolorised dye and initial amount of dye) by a free enzyme was 48 % and the decolorisation rate was determined at 2.11 × 10−3 mgdye mg −1enz min−1. After immobilisation in lens-shaped poly(vinyl alcohol) hydrogel capsules LentiKats® Biocatalyst (LB) (concentration of immobilised enzyme: 4 mg per g of particles; volume-loading rate of LB: 10 g per 100 mL of medium), the enzyme retained 16.1 % of its original activity (1.34 U mg −1enz ). Immobilised laccase was used for the dye decolorisation in 130 repeated batch tests with 71 % efficiency (LB activity: 7 × 10−3 mgdye min−1 g −1LB ). In continuous mode (after 716.5 h), the efficiency of the dye decolorisation was 48 % (LB activity: 3.3 × 10−4 mgdye min/−1 g −1LB ).

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  1. LentiKat’s a.s., Pod Vinicí 83, 471 27, Stráž pod Ralskem, Czech Republic

    Radek Stloukal, Jarmila Watzková & Barbora Gregušová

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  1. Radek Stloukal
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  2. Jarmila Watzková
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  3. Barbora Gregušová
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Correspondence to Radek Stloukal.

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Stloukal, R., Watzková, J. & Gregušová, B. Dye decolorisation by laccase immobilised in lens-shaped poly(vinyl alcohol) hydrogel capsules. Chem. Pap. 68, 1514–1520 (2014). https://doi.org/10.2478/s11696-014-0601-3

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