Chemical composition and antioxidant activity of sulphated polysaccharides extracted from Fucus vesiculosus using different hydrothermal processes
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Sulphated polysaccharides (SP) were extracted from Fucus vesiculosus seaweed by using two different hydrothermal processes: microwave-assisted extraction (MAE) and autohydrolysis (AH). The extraction yields, chemical composition, and antioxidant activity of the polysaccharides extracted were determined and compared. Although both processes afforded SP with similar yields (18.2 mass % and 16.5 mass %, for MAE and AH, respectively) and l-fucose as the main monosaccharide, the heterogeneous structure of the polysaccharide recovered was significantly affected by the AH process. The SP obtained by MAE contained 53.8 mole % of fucose, 35.3 mole % of xylose, and 10.8 mole % of galactose; while the SP obtained by AH was composed of 76.8 mole % of fucose and 23.2 mole % of galactose. Both samples presented comparable values of antioxidant activity by the di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (2,2-diphenyl-1-picrylhydrazyl, DPPH), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and lipid oxidation inhibition methods, but the polysaccharide obtained by AH exhibited a higher antioxidant potential by the differential pulse voltammetry technique. This study demonstrates that the chemical composition and antioxidant activity of SP obtained from F. vesiculosus vary according to the process used for their extraction. However, the SP obtained by MAE or AH both have the potential for use as natural antioxidants in industrial applications.
Keywordsantioxidant activity autohydrolysis fucan Fucus vesiculosus microwave-assisted extraction sulphated polysaccharides
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- Barros, L., Falcão, S., Baptista, P., Freire, C., Vilas-Boas, M., & Ferreira, I. C. F. R. (2008). Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chemistry, 111, 61–66. DOI:10.1016/j.foodchem.2008.03.033.Google Scholar
- Bhakuni, D. S., & Rawat, D. S. (2005). Bioactive marine natural products. New York, NY, USA: Springer.Google Scholar
- Costa, L. S., Fidelis, G. P., Cordeiro, S. L., Oliveira, R. M., Sabry, D. A., Câmara, R. B. G., Nobre, L. T. D. B., Costa, M. S. S. P., Almeida-Lima, J., Farias, E. H. C., Leite, E. L., & Rocha, H. A. O. (2010). Biological activities of sulfated polysaccharides from tropical seaweeds. Biomedicine & Pharmacotherapy, 64, 21–28. DOI: 10.1016/j.biopha.2009.03.005.CrossRefGoogle Scholar
- Dodgson, K. S. (1961). Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochemical Journal, 78, 312–319.Google Scholar
- Jiao, G. L., Yu, G. L., Wang, W., Zhao, X. L., Zhang, J. Z., & Ewart, S. H. (2012). Properties of polysaccharides in several seaweeds from Atlantic Canada and their potential anti-influenza viral activities. Journal of Ocean University of China, 11, 205–212. DOI: 10.1007/s11802-012-1906-x.CrossRefGoogle Scholar
- Kim, D. O., & Lee, C. Y. (2002). Extraction and isolation of polyphenolics. Current Protocols in Food Analytical Chemistry, 6, I1.2.1–I1.2.12. DOI: 10.1002/0471142913.fai0102s06.Google Scholar
- Wang, J., Zhang, Q. B., Zhang, Z. S., Song, H. F., & Li, P. C. (2010). Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 46, 6–12. DOI: 10.1016/j.ijbiomac.2009.10.015.CrossRefGoogle Scholar
- Yuan, H. M., Zhang, W. W., Li, X. G., Lü, X. X., Li, N., Gao, X. L., & Song, J. M. (2005). Preparation and in vitro antioxidant activity of κ-carrageenan oligosaccharides and their oversulfated, acetylated, and phosphorylated derivatives. Carbohydrate Research, 340, 685–692. DOI: 10.1016/j.carres.2004.12.026.CrossRefGoogle Scholar