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Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

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Abstract

Benzo[c]phenanthridine alkaloid sanguilutine was extracted and purified from the dried roots of Sanguinaria canadensis. The interaction of the positively charged iminium form of alkaloid with double-stranded DNA oligonucleotides was studied using luminescence spectroscopy. The results showed that the interaction with various double-stranded oligonucleotides was not specific to A-T or G-C base pairs; also, no preference was found for either homogeneous or heterogeneous base composition of strands. The association constants were calculated to be in the range of (1.31–14.36) × 105 M−1. The luminescence intensity response at 610 nm to low concentrations of double-stranded DNA was found to be linear and can potentially be used for the fluorometric quantification of DNA. The limit of detection was estimated to be 120 ng mL−1 of DNA (calculated by 3σ method).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic

    Stanislava Rybáková & Jana Urbanová

  2. Central European Institute of Technology CEITEC MU, Masaryk University, Kamenice 2, 61137, Brno, Czech Republic

    Michal Rájecký & Petr Táborský

  3. Department of Chemistry and Biochemistry, Faculty of Medicine, Masaryk University, Kamenice 2, 61137, Brno, Czech Republic

    Kristýna Pěnčíková & Eva Táborská

  4. Department of Analytical Chemistry, University of Barcelona, Diagonal 645, 08028, Barcelona, Spain

    Raimundo Gargallo

Authors
  1. Stanislava Rybáková
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  2. Michal Rájecký
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  3. Jana Urbanová
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  4. Kristýna Pěnčíková
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  5. Eva Táborská
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  6. Raimundo Gargallo
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  7. Petr Táborský
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Correspondence to Petr Táborský.

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Rybáková, S., Rájecký, M., Urbanová, J. et al. Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine. Chem. Pap. 67, 568–572 (2013). https://doi.org/10.2478/s11696-013-0340-x

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  • DOI: https://doi.org/10.2478/s11696-013-0340-x

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