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Effects of enzymes and hydrocolloids on physical, sensory, and shelf-life properties of wheat bread

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Abstract

This study compares two types of enzymes: maltogenic amylase (Novamyl 10000 BG) and lipase (Lipopan F BG, Lipopan Xtra BG), both separately and in combination, and seven hydrocolloids (guar gum, xanthan gum, carrageenan, β-glucan, carob gum, and carboxymethyl cellulose (CMC)) on the physical and sensory properties of a bakery product (white wheat bread). Their effect was observed on the baking characteristics of volume, specific volume, shape, mass, shelf-life (penetration and water activity test), and overall taste. The best results in shelf-life extension from the hydrocolloids were shown by β-glucan, a combination of xanthan gum + carrageenan and guar gum. From the enzymes, the best results were achieved with Lipopan Xtra BG and Novamyl 10000 BG + Lipopan F BG combination. The sensory properties (e.g. taste, colour, shape, aroma, elasticity, hardness) were evaluated by ten trained panellists, holding certification in sensory analysis. β-Glucan and Novamyl 10000 BG + Lipopan F BG combination increased the bread volume significantly and also were deemed to afford the most favourable taste.

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References

  • AACC (2000). Approved methods of the American Association of Cereal Chemists (10th ed.). St. Paul, MN, USA: AACC Press.

    Google Scholar 

  • Andronic, D., Bulancea, M., Dabija, A., & Miron, A. (2007). Researches concerning the influence of some commercial enzymatic preparats over the quality of frozen sheet dough. Journal of Agroalimentary Processes and Technologies, 13, 381–386.

    CAS  Google Scholar 

  • Caballero, P. A., Gómez, M., & Rosell, C. M. (2007). Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination. Journal of Food Engineering, 81, 42–53. DOI: 10.1016/j.jfoodeng.2006.10.007.

    Article  CAS  Google Scholar 

  • Christiansen, L., Vind, J., Borch, K., & Heldt-Hansen, H. (2003). Generation of lipases with different specificities and functionalities in breadmaking. In C. M. Courtin, V. S. Veraverbeke, & J. A. Delcour (Eds.), Proceedings of the Third European Symposium on Enzymes in Grain Processing: Recent Advances in Enzymes in Grain Processing (pp. 269–274). Leuven, Belgium: Katholieke Universiteit Leuven.

    Google Scholar 

  • Frank, P. (2008). Fresh-keeping technology builds brand equity. Baking Management (Issue November 1, 2008). Retrieved from http://baking-management.com/rd_applications/freshkeeping_technology_builds_1108/index1.html

  • Frost & Sullivan (2004). Bakery enzymes: The volume enhancers in the food enzyme industry. Retrieved from http://www.frost.com/sublib/display-market-insight-top.do?id=17170372

  • Gray, J. A., & Bemiller, J. N. (2003). Bread staling: Molecular basis and control. Comprehensive Reviews in Food Science and Food Safety, 2, 1–21. DOI: 10.1111/j.1541-4337.2003.tb00011.x.

    Article  CAS  Google Scholar 

  • Grosch, W., & Wieser, H. (1999). Redox reactions in wheat dough as affected by ascorbic acid. Journal of Cereal Science, 29, 1–16. DOI: 10.1006/jcrs.1998.0218.

    Article  CAS  Google Scholar 

  • Guarda, A., Rosell, C. M., Benedito, C., & Galotto, M. J. (2004). Different hydrocolloids as bread improvers and antistaling agents. Food Hydrocolloids, 18, 241–247. DOI: 10.1016/s0268-005x(03)00080-8.

    Article  CAS  Google Scholar 

  • Houde, A., Kademi, A., & Leblanc, D. (2004). Lipases and their industrial applications: An overview. Applied Biochemistry and Biotechnology, 118, 155–170. DOI: 10.1385/abab:118:1-3:155.

    Article  CAS  Google Scholar 

  • Kohajdová, Z., & Karovičová, J. (2008). Influence of hydrocolloids on quality of baked goods. Acta Scientiarum Polonorum, Technologia Alimentaria, 7, 43–49.

    Google Scholar 

  • Kohajdová, Z., & Karovičová, J. (2010). Impact of potassium iodate on the quality of wheat-spelt baked goods. Acta Scientiarum Polonorum, Technologia Alimentaria, 9, 443–450.

    Google Scholar 

  • Mandala, I., Karabela, D., & Kostaropoulos, A. (2007). Physical properties of breads containing hydrocolloids stored at low temperature. I. Effect of chilling. Food Hydrocolloids, 21, 1397–1406. DOI: 10.1016/j.foodhyd.2006.11.007.

    Article  CAS  Google Scholar 

  • Mikuš, Ł., Kocková, M., Dodok, L., & Kováčová, M. (2011a). Application of selected enzymes to improve shelf-life and rheological properties of bakery products. In D. Koceva Komlenić (Ed.), Book of Abstracts of the 6th International Congress FLOUR-BREAD’ 11, October 12–14, 2011 (pp. 66). Osijek, Croatia: University of Osijek.

    Google Scholar 

  • Mikuš, Ł., Valík, Ł., & Dodok, L. (2011b). Usage of hydrocolloids in cereal technology. Acta Universitatis Agriculturae et Silviculturae Mendeleianae Brunensis, 59, 325–333.

    Google Scholar 

  • Moayedallaie, S., Mirzaei, M., & Paterson, J. (2010). Bread improvers: Comparison of a range of lipases with a traditional emulsifier. Food Chemistry, 122, 495–499. DOI: 10.1016/j.foodchem.2009.10.033.

    Article  CAS  Google Scholar 

  • Mondal, A., & Datta, A. K. (2008). Bread baking — A review. Journal of Food Engineering, 86, 465–474. DOI: 10.1016/j.jfoodeng.2007.11.014.

    Article  Google Scholar 

  • Novasina AG (1999). Novasina AW Sprint for measuring water activity. Pfäffikon, Switzerland: Novasina Axair.

    Google Scholar 

  • Phillips, G. O., & Williams, P. A. (Eds.) (2000). Handbook of hydrocolloids. Cambridge, UK: Woodhead Publishing Limited.

    Google Scholar 

  • Primo-Martín, C., Hamer, R. J., & de Jongh, H. H. J. (2006). Surface layer properties of dough liquor components: Are they key parameters in gas retention in bread dough? Food Biophysics, 1, 83–93. DOI: 10.1007/s11483-006-9008-1.

    Article  Google Scholar 

  • Purhagen, J. K., Sjöö, M. E., & Eliasson, A. C. (2011). Starch affecting anti-staling agents and their function in freestanding and pan-baked bread. Food Hydrocolloids, 25, 1656–1666. DOI: 10.1016/j.foodhyd.2011.03.004

    Article  CAS  Google Scholar 

  • Renard, A. C. (1996). Ultra-fresh yoghurt or dessert. Revue Laiti`ere Fran?caise, 555, 12–14.

    Google Scholar 

  • Ribotta, P. D., Pérez, G. T., León, A. E., & Añón, M. C. (2004). Effect of emulsifier and guar gum on micro structural, rheological and baking performance of frozen bread dough. Food Hydrocolloids, 18, 305–313. DOI: 10.1016/s0268-005x(03) 00086-9.

    Article  CAS  Google Scholar 

  • Rogers, D. E., Zeleznak, K. J., Lai, C. S., & Hoseney, R. C. (1988). Effect of native lipids, shortening, and bread moisture on bread firming. Cereal Chemistry, 65, 398–401.

    CAS  Google Scholar 

  • Rollet, B. (1995). Stabilizers for dairy desserts: Interesting synergism. Revue Laiti`ere Française, 555, 22–23.

    Google Scholar 

  • Rosell, C. M., Rojas, J. A., & Benedito de Barber, C. (2001). Influence of hydrocolloids on dough rheology and bread quality. Food Hydrocolloids, 15, 75–81. DOI: 10.1016/s0268-005x(00)00054-0.

    Article  CAS  Google Scholar 

  • Sanz Penella, J. M., Collar, C., & Haros, M. (2008). Effect of wheat bran and enzyme addition on dough functional performance and phytic acid levels in bread. Journal of Cereal Science, 48, 715–721. DOI: 10.1016/j.jcs.2008.03.006.

    Article  CAS  Google Scholar 

  • Shittu, T. A., Aminu, R. A., & Abulude, E. O. (2009). Functional effects of xanthan gum on composite cassava-wheat dough and bread. Food Hydrocolloids, 23, 2254–2260. DOI: 10.1016/j.foodhyd.2009.05.016.

    Article  CAS  Google Scholar 

  • Skendi, A., Papageorgiou, M., & Biliaderis, C. G. (2009). Effect of barley β-glucan molecular size and level on wheat dough rheological properties. Journal of Food Engineering, 91, 594–601. DOI: 10.1016/j.jfoodeng.2008.10.009.

    Article  CAS  Google Scholar 

  • Skendi, A., Biliaderis, C. G., Papageorgiou, M., & Izydorczyk, M. S. (2010). Effects of two barley β-glucan isolates on wheat flour dough and bread properties. Food Chemistry, 119, 1159–1167. DOI: 10.1016/j.foodchem.2009.08.030.

    Article  CAS  Google Scholar 

  • Sozer, N. (2009). Rheological properties of rice pasta dough supplemented with proteins and gums. Food Hydrocolloids, 23, 849–855. DOI: 10.1016/j.foodhyd.2008.03.016.

    Article  CAS  Google Scholar 

  • Stojceska, V., & Ainsworth, P. (2008). The effect of different enzymes on the quality of high-fibre enriched brewer’s spent grain breads. Food Chemistry, 110, 865–872. DOI: 10.1016/j.foodchem.2008.02.074.

    Article  CAS  Google Scholar 

  • van der Maarel, M. J. E. C, van der Veen, B., Uitdehaag, J. C. M., Leemhuis, H., & Dijkhuizen, L. (2002). Properties and applications of starch-converting enzymes of the α-amylase family. Journal of Biotechnology, 94, 137–155. DOI: 10.1016/s0168-1656(01)00407-2.

    Article  Google Scholar 

  • Wagner, M. J., Lucas, T., Le Ray, D., & Trystram, G. (2007). Water transport in bread during baking. Journal of Food Engineering, 78, 1167–1173. DOI: 10.1016/j.jfoodeng.2005.12.029.

    Article  Google Scholar 

  • Wang, X., Choi, S. G., & Kerr, W. L. (2004). Water dynamics in white bread and starch gels as affected by water and gluten content. LWT-Food Science and Technology, 37, 377–384. DOI: 10.1016/j.lwt.2003.10.008.

    Article  Google Scholar 

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Mikuš, Ľ., Kováčová, M., Dodok, L. et al. Effects of enzymes and hydrocolloids on physical, sensory, and shelf-life properties of wheat bread. Chem. Pap. 67, 292–299 (2013). https://doi.org/10.2478/s11696-012-0286-4

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  • DOI: https://doi.org/10.2478/s11696-012-0286-4

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