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Immobilization in biotechnology and biorecognition: from macro- to nanoscale systems

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Abstract

Biological molecules such as enzymes, cells, antibodies, lectins, peptide aptamers, and cellular components in an immobilized form are extensively used in biotechnology, in biorecognition and in many medicinal applications. This review provides a comprehensive summary of the developments in new immobilization materials, techniques, and their practical applications previously developed by the authors. A detailed overview of several immobilization materials and technologies is given here, including bead cellulose, encapsulation in ionotropic gels and polyelectrolyte complexes, and various immobilization protocols applied onto surfaces. In addition, the review summarises the screening and design of an immobilization protocol, practical applications of immobilized biocatalysts in the industrial production of metabolites, monitoring, and control of fermentation processes, preparation of electrochemical/optical biosensors and biofuel cells.

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Authors and Affiliations

  1. Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia

    Marek Bučko, Danica Mislovičová, Jozef Nahálka, Alica Vikartovská, Jana Šefčovičová, Jaroslav Katrlík, Ján Tkáč & Peter Gemeiner

  2. Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41, Bratislava, Slovakia

    Igor Lacík

  3. Axxence Slovakia s.r.o., Poštová 503/1, 81106, Bratislava, Slovakia

    Vladimír Štefuca

  4. Institute of Chemical and Environmental Engineering, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Milan Polakovič

  5. Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Michal Rosenberg, Martin Rebroš, Daniela Šmogrovičová & Juraj Švitel

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  1. Marek Bučko
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Correspondence to Peter Gemeiner.

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Bučko, M., Mislovičová, D., Nahálka, J. et al. Immobilization in biotechnology and biorecognition: from macro- to nanoscale systems. Chem. Pap. 66, 983–998 (2012). https://doi.org/10.2478/s11696-012-0226-3

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