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Liquid chromatographic determination of meloxicam in serum after solid phase extraction

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Abstract

A liquid chromatographic method for the determination of meloxicam in serum has been developed. The technique includes a solid phase extraction of the serum samples on [poly (divinylbenzeneco-N-vinylpyrrolidone)] as a solid phase extraction sorbent. After conditioning, the cartridge was loaded with 1 mL of acidified serum containing an internal standard. Elution was carried out using 1 mL of water-acetonitrile (φ r = 1: 1) mixture. After evaporation of the eluate to dryness and reconstitution of the residue with 0.1 mL of methanol, the samples were analyzed on a Symmetry C18 column. Mobile phase consisted of 1 % aqueous acetic acid/THF/acetonitrile (φ r = 60: 30: 10) + 0.1 mL of 1-octane sulfonic acid. Detection was carried out using a photodiode array detector. Full validation of the proposed method is provided. Linearity of the method was proven over the range of 0.01–10 φg mL−1 of meloxicam. Meloxicam assay was accurate and reliable with average intra- and inter-day precisions lower than 5.0 % and the intra- and inter-day accuracy higher than 97 %. Limits of detection (LOD) and quantitation (LOQ) found were 0.003 μg mL−1 and 0.01 μg mL−1, respectively. The proposed method was successfully utilized to quantify meloxicam in serum.

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References

  • Altinőz, S., Nemutlu E., & Kır, S. (2002). Polarographic behaviour of meloxicam and its determination in tablet preparations and spiked plasma. Il Farmaco, 57, 463–468. DOI: 10.1016/S0014-827X(02)01239-9.

    Article  Google Scholar 

  • Altiokka, G., Atkosar, Z., & Tuncel, M. (2001). Pulse polarographic determination of meloxicam. Pharmazie, 56, 184–185.

    CAS  Google Scholar 

  • Bae, J.-W., Kim, M.-J., Jang, C.-G., & Lee, S.-Y. (2007). Determination of meloxicam in human plasma using a HPLC method with UV detection and its application to a pharmacokinetic study. Journal of Chromatography B, 859, 69–73. DOI: 10.1016/j.jchromb.2007.09.004.

    Article  CAS  Google Scholar 

  • Bebawy, L. I. (1998). Stability-indicating method for the determination of meloxicam and tetracaine hydrochloride in the presence of their degradation products. Spectroscopy Letters, 31, 797–820. DOI: 10.1080/00387019808007401.

    Article  CAS  Google Scholar 

  • Beltagi, A. M., Ghoneim, M. M., & Radi, A. (2002). Electrochemical reduction of meloxicam at mercury electrode and its determination in tablets. Journal of Pharmaceutical and Biomedical Analysis, 27, 795–801. DOI: 10.1016/S0731-7085(01)00528-3.

    Article  CAS  Google Scholar 

  • Busch, U., Schmid, J., Heinzel, G., Schmaus, H., Baierl, J., Huber, C., & Roth, W. (1998). Pharmacokinetics of meloxicam in animals and the relevance to humans. Drug Metabolism and Disposition, 26, 576–584.

    CAS  Google Scholar 

  • Dasandi, B., Shivaprakash, Saroj, H., & Bhat, K. M. (2002). LC determination and pharmacokinetics of meloxicam. Journal of Pharmaceutical and Biomedical Analysis, 28, 999–1004. DOI: 10.1016/S0731-7085(02)00064-X.

    Article  CAS  Google Scholar 

  • García, M. S., Sánchez-Pedreño, C., Albero, M. I., & Martí, J. (2000). Spectrophotometric methods for determining meloxicam in pharmaceuticals using batch and flow-injection procedures. European Journal of Pharmaceutical Sciences, 9, 311–316. DOI: 10.1016/S0928-0987(99)00069-X.

    Article  Google Scholar 

  • Hassan, E. M. (2002). Spectrophotometric and fluorimetric methods for the determination of meloxicam in dosage forms. Journal of Pharmaceutical and Biomedical Analysis, 27, 771–777. DOI: 10.1016/S0731-7085(01)00530-1.

    Article  CAS  Google Scholar 

  • Igualada, C., Moragues, F., & Pitarch, J. (2007). Rapid method for the determination of non-steroidal anti-inflammatory drugs in animal tissue by liquid chromatography-mass spectrometry with ion-trap detector. Analytica Chimica Acta, 586, 432–439. DOI: 10.1016/j.aca.2006.10.040.

    Article  CAS  Google Scholar 

  • Joseph-Charles, J., & Bertucat, M. (1999). Determination of meloxicam in tablet formulations by ultraviolet spectrophotometry and high-performance liquid chromatography. Analytical Letters, 32, 2051–2059. DOI: 10.1080/00032719908542951.

    Article  CAS  Google Scholar 

  • Luger, P., Daneck, K., Engel, W., Trummlitz, G., & Wagner, K. (1996). Structure and physicochemical properties of meloxi cam, a new NSAID. European Journal of Pharmaceutical Sciences, 4, 175–187. DOI: 10.1016/0928-0987(95)00046-1.

    Article  CAS  Google Scholar 

  • Nageswara Rao, R., Meena, S., & Raghuram Rao, A. (2005). An overview of the recent developments in analytical methodologies for determination of COX-2 inhibitors in bulk drugs, pharmaceuticals and biological matrices. Journal of Pharmaceutical and Biomedical Analysis, 39, 349–363. DOI: 10.1016/j.jpba.2005.03.040.

    Article  CAS  Google Scholar 

  • Nemutlu, E., & Kir, S. (2003). Method development and validation for the analysis of meloxicam in tablets by CZE. Journal of Pharmaceutical and Biomedical Analysis, 31, 393–400. DOI: 10.1016/S0731-7085(02)00641-6.

    Article  CAS  Google Scholar 

  • Noble, S., & Balfour, J. A. (1996). Meloxicam. Drugs, 51, 424–443.

    Article  CAS  Google Scholar 

  • Radi, A., El Ries, M. A., El-Anwar, F., & El-Sherif, Z. (2001a). Electrochemical oxidation of meloxicam and its determination in tablet dosage form. Analytical Letters, 34, 739–748. DOI: 10.1081/AL-100103216.

    Article  CAS  Google Scholar 

  • Radi, A.-E, Ghoneim, M., & Beltagi, A. (2001b). Cathodic adsorptive stripping square-wave voltammetry of the anti-inflammatory drug meloxicam. Chemical & Pharmaceutical Bulletin, 49, 1257–1260. DOI: 10.1248/cpb.49.1257.

    Article  CAS  Google Scholar 

  • Rigato, H. M., Duarte Mendes, G., Carter do Carmo Borges, N., & Moreno, R. A. (2006). Meloxicam determination in human plasma by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS) in Brazilian bioequivalence studies. International Journal of Clinical Pharmacology and Therapeutics, 44, 489–498.

    CAS  Google Scholar 

  • Sener, E., Altiokka, G., Atkosar, Z., & Tuncel, M. (2001). Flow injection analysis of meloxicam using UV-detection. Pharmazie, 56, 186–187.

    CAS  Google Scholar 

  • Shukla, M., Singh, G., Sindhura, B. G., Telang, A. G., Rao, G. S., & Malik, J. K. (2007). Comparative plasma pharmacokinetics of meloxicam in sheep and goats following intravenous administration. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 145, 528–532. DOI: 10.1016/j.cbpc.2007.01.020.

    Article  Google Scholar 

  • Tsai, R.-S., Carrupt, P.-A., El Tayar, N., Giroud, Y., Andrade, E., Testa, B., Breé, F., & Tillement, L.-P. (1993). Physicochemical and structural properties of non-steroidal anti-inflammatory oxicams. Helvetica Chimica Acta, 76, 842–854. DOI: 10.1002/hlca.19930760208.

    Article  CAS  Google Scholar 

  • Türck, D., Roth, W., & Busch, U. (1996). A review of the clinical pharmacokinetics of meloxicam. British Journal of Rheumatology, 35, 13–16.

    Google Scholar 

  • Velpandian, T., Jaiswal, J., Bhardwaj, R. K., & Gupta, S. K. (2000). Development and validation of a new high-performance liquid chromatographic estimation method of meloxicam in biological samples. Journal of Chromatography B: Biomedical Sciences and Applications, 738, 431–436. DOI: 10.1016/S0378-4347(99)00537-X.

    Article  CAS  Google Scholar 

  • Wiesner, J. L., de Jager, A. D., Sutherland, F. C. W., Hundt, H. K. L., Swart, K. J., Hundt, A. F., & Els, J. (2003). Sensitive and rapid liquid chromatography-tandem mass spectrometry method for the determination of meloxicam in human plasma. Journal of Chromatography B, 785, 115–121. DOI: 10.1016/S1570-0232(02)00862-0.

    Article  CAS  Google Scholar 

  • Yuan, Y., Chen, X., & Zhong, D. (2007). Determination of meloxicam in human plasma by liquid chromatography-tandem mass spectrometry following transdermal administration. Journal of Chromatography B, 852, 650–654. DOI: 10.1016/j.jchromb.2007.01.036.

    Article  CAS  Google Scholar 

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  1. Laboratoire de chromatographie, Faculté de Chimie, USTHB, B.P. no32, El-Alia, Bab-Ezzouar, 96100, Alger, Algeria

    Radia Ouarezki & Moulay-Hassane Guermouche

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  1. Radia Ouarezki
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  2. Moulay-Hassane Guermouche
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Correspondence to Moulay-Hassane Guermouche.

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Ouarezki, R., Guermouche, MH. Liquid chromatographic determination of meloxicam in serum after solid phase extraction. Chem. Pap. 64, 429–433 (2010). https://doi.org/10.2478/s11696-010-0031-9

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