Chemical Papers

, Volume 64, Issue 4, pp 443–449 | Cite as

Preparation and properties of surfactant-bacillolysin ion-pair in organic solvents

  • Ying ZhangEmail author
  • Jiadong Gu
  • Shugen Wang
  • Xuerong Fan


The transesterification-active enzyme bacillolysin was extracted into organic solvents such as isooctane by enzyme-AOT (bis (2-ethylhexyl) sulfosuccinate) ion-pairing preserving its natural second structure and catalytic activity. Extraction efficiency was affected by the interaction mode of the two phases, ionic strength, and pH of aqueous phase, surfactant and enzyme concentration. Magnetic stirring with phase mixing was favorable for the enzyme extraction. Optimal ionic strength and pH were 8 mM CaCl2 and 5.0, respectively. Critical number of AOT molecule for an enzyme molecule to be extracted into isooctane was 89. Optimal initial enzyme concentration in the aqueous phase was 7 mg mL−1 while the initial AOT concentration in isooctane was 3 mM. Within CMC (critical micellar concentration) of AOT in isooctane, the increase of initial AOT concentration enhanced the extraction efficiency.


bacillolysin AOT ion-pair solvent transesterification 


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2010

Authors and Affiliations

  • Ying Zhang
    • 1
    Email author
  • Jiadong Gu
    • 1
  • Shugen Wang
    • 1
  • Xuerong Fan
    • 1
  1. 1.Key Laboratory of Eco-Textiles, School of Textiles and ClothingJiangnan UniversityWuxiChina

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