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Characterization of recombinant antibodies for detection of TNT and its derivatives

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Abstract

Diverse recombinant immunoreagents specific for TNT-derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP-protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT-analog TNP-Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.

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Authors and Affiliations

  1. Institute of General and Physical Chemistry, Studentski Trg 12-16, 11000, Belgrade, Serbia

    Mladen Simonoviíc & Branislav Simonović

  2. Faculty of Mathematics and Natural Sciences, University of Kragujevac, Radoja Domanovića 12, 34000, Kragujevac, Serbia

    Svetlana Milosević-Zlatanović

  3. Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, 11000, Belgrade, Serbia

    Nenad Milosavić

  4. Faculty of Chemistry, University of Belgrade, Studentski Trg 12-16, 11000, Belgrade, Serbia

    Miroslav M. Vrvić

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  1. Mladen Simonoviíc
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  2. Svetlana Milosević-Zlatanović
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  3. Nenad Milosavić
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  4. Miroslav M. Vrvić
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  5. Branislav Simonović
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Correspondence to Mladen Simonoviíc.

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Simonoviíc, M., Milosević-Zlatanović, S., Milosavić, N. et al. Characterization of recombinant antibodies for detection of TNT and its derivatives. Chem. Pap. 63, 391–398 (2009). https://doi.org/10.2478/s11696-009-0043-5

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  • DOI: https://doi.org/10.2478/s11696-009-0043-5

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