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Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system

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Abstract

A sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from 0.4 ng mL−1 to 400.0 ng mL−1 (r 2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals, human urine, and serum. Possible mechanism of the reaction was also discussed.

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Authors and Affiliations

  1. Department of Chemistry, Northwest University, Xi’an, 710069, China

    X. D. Shao, H. Y. Liu, X. F. Gao, W. Q. Chen & Z. H. Song

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  1. X. D. Shao
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  2. H. Y. Liu
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  3. X. F. Gao
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  4. W. Q. Chen
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  5. Z. H. Song
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Correspondence to Z. H. Song.

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Shao, X.D., Liu, H.Y., Gao, X.F. et al. Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system. Chem. Pap. 61, 353–358 (2007). https://doi.org/10.2478/s11696-007-0047-y

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  • DOI: https://doi.org/10.2478/s11696-007-0047-y

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