First Report of Molecular Characterization of Oochoristica hemidactyli Johri, 1955 (Cestoda, Linstowiidae) from the Common Wall Lizard, Hemidactylus brooki Gray, 1845 (Reptilia: Gekkonidae) Along with Morphological Redescription

Abstract

Introduction

During an investigation, a rare cestode species belonging to the genus, Oochoristica were found in Meerut, UP, India, from the intestine of the host, Hemidactylus brookii Gray, 1845 (Reptilia: Gekkonidae). The tapeworms were found to be similar in their morphological characters with Oochoristica hemidactyli Johri, 1955 (Cestoda: Linstowiidae).

Materials and Methods

The host Hemidactylus brookii used in this analysis was caught from May to July 2018 from Department of Zoology, Chaudhary Charan Singh University, Meerut (UP), India. Parasites were examined by microscopy. 18S and ITS1-5.8S-ITS2 region of ribosomal RNA were sequenced and analyzed for the molecular study.

Results

This paper provides the first trustworthy confirmation about the occurrence of O. hemidactyli inferred with molecular data using ribosomal 18S and ITS1-5.8S-ITS2 gene cluster DNA sequences. Molecular analysis shows that the studied specimens were clustered separately and did not show a close relationship with any other cestode spp., represented in the GenBank.

Conclusion

This is the foremost molecular characterization of any species of Oochoristica and of O. hemidactyli after 64 years of its original description that contributing to the systematic analysis of the helminthological biodiversity of reptilian hosts. These results provide a reference for future molecular study of species of Oochoristica.

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References

  1. 1.

    de Chambrier A, Waeschenbach A, Fisseha M, Scholz T, Mariaux J (2015) A large 28S rDNA-based phylogeny confirms the limitations of established morphological characters for classification of proteocephalidean tapeworms (Platyhelminthes, Cestoda). Zookeys. https://doi.org/10.3897/zookeys.500.9360

    Article  PubMed  PubMed Central  Google Scholar 

  2. 2.

    Galazzo DE, Dayanandan S, Marcogliese DJ, McLaughlin JD (2002) Molecular systematics of some North American species of Diplostomum (Digenea) based on rDNA-sequence data and comparisons with European congeners. Can J Zool 80:2207–2217. https://doi.org/10.1139/z02-198

    CAS  Article  Google Scholar 

  3. 3.

    Gray JE (1845) Catalogue of the specimens of lizards in the collection of the British Museum, vol 27. Edward Newman, London, p 289

    Google Scholar 

  4. 4.

    Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  5. 5.

    Johri GN (1955) On a new cestode, Oochoristica hemidactyli from the common wall lizard, Hemidactylus flaviviridis Rüppell. Proc Indian Acad Sci Sect B 41:79–81

    Google Scholar 

  6. 6.

    Littlewood DTJ, Olson PD (2001) Small subunit rDNA and the phylum Platyhelminthes: signal, noise, conflict and compromise. In: Littlewood DTJ, Bray RA (eds) Interrelationships of the Platyhelminthes. Taylor & Francis, London, pp 262–278

    Google Scholar 

  7. 7.

    Lühe M (1998) Oochoristica nov. gen. Taeniadarum (Vorläufige Mitteilung). Zool Anz 21:650–652

    Google Scholar 

  8. 8.

    McAllister CT, Bursey CR (2017) A new species of Oochoristica (Cyclophyllidea: Linstowiidae) from non-native Mediterranean geckos, Hemidactylus turcicus (Sauria: Gekkonidae), from Texas, USA. Acta Parasitol 62:296–305. https://doi.org/10.1515/ap-2017-0037

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Milne I, Lindner D, Bayer M, Husmeier D, McGuire G, Marshall DF, Wright F (2009) TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics 25:126–127. https://doi.org/10.1093/bioinformatics/btn575

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818. https://doi.org/10.1093/bioinformatics/14.9.817

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolution genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680. https://doi.org/10.1093/nar/22.22.4673

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We would like to acknowledge the Head, Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing laboratory facilities.

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Correspondence to Anshu Chaudhary.

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The authors declare that they have no conflict of interest.

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All experiments and handling of host were according to the animal welfare guidelines and recommendations under Committee for the Purpose of Control And Supervision of Experiments on Animals (CPCSEA), India.

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Verma, C., Chaudhary, A., Sharma, B. et al. First Report of Molecular Characterization of Oochoristica hemidactyli Johri, 1955 (Cestoda, Linstowiidae) from the Common Wall Lizard, Hemidactylus brooki Gray, 1845 (Reptilia: Gekkonidae) Along with Morphological Redescription. Acta Parasit. 65, 250–255 (2020). https://doi.org/10.2478/s11686-019-00129-6

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Keywords

  • Oochoristica hemidactyli
  • Cestode
  • Hemidactylus brookii
  • 18S
  • ITS1-5.8S-ITS2
  • India