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Biologia

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Genetic stability assessment of Taraxacum pieninicum plantlets after long-term slow growth storage using ISSR and SCoT markers

  • Monika Kamińska
  • Andrzej Tretyn
  • Alina TrejgellEmail author
Original Article
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Abstract

Genetic stability is highly important in terms of endangered species gene banks. Culture and cold stresses under in vitro conditions may lead to genetic variability. Many methods and possibilities to design appropriate starters for DNA-fingerprinting purposes increase cost and time consumption. Furthermore, multiplicity of possible primers makes it difficult to standardize plant research. The aim of this study was to verify effectiveness of various methods in assessing the clonal homogeneity of Taraxacum pieninicum plantlets regenerated after long-term in vitro cold-storage and to simplify the selection of the genetic stability analysis for Asteraceae family. Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT) polymorphism assays were performed to detect DNA sequence variation. No differences were observed using 16 ISSR markers and 12 SCoT markers between regrown plantlets after storage and plants cultivated from seeds in a soil. Furthermore, SCoT markers were most effective for screening T. pieninicum genome and appeared to be highly useful for different micropropagated and endangered species of Asteraceae family.

Keywords

Asteraceae Cold storage Endangered species Micropropagation Molecular markers 

Abbreviations

AAD

Arbitrarily Amplified Dominant

AFLP

Amplified Fragment Length Polymorphism

IRAP

Inter-Retrotransposon Amplified Polymorphism

ISSR

Inter Simple Sequence Repeats

MSAP

Methylation Sensitive Amplified Polymorphism

QTL

Qualitative Trait Loci

RAPD

Random Amplified Polymorphic DNA

REMAP

Retrotransposon-Microsatellite Amplified Polymorphism

RFLP

Restriction Fragment Length Polymorphism

ScoT

Start Codon Targeted

S-SAP

Sequence-Specific Amplified Polymorphism

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Notes

Acknowledgments

This project was supported by funds from Ministry of Science and Higher Education (PL) for young scientists from Nicolaus Copernicus University in Toruń (2827-B).

Author contributions

Monika Kamińska designed and carried out all the experiments, analyzed the data and wrote the manuscript, Alina Trejgell helped in analysis of the data and in writing the manuscript, and Andrzej Tretyn helped in preparing the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Monika Kamińska
    • 1
  • Andrzej Tretyn
    • 1
  • Alina Trejgell
    • 1
    Email author
  1. 1.Department of Plant Physiology and BiotechnologyNicolaus Copernicus UniversityToruńPoland

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