pp 1–7 | Cite as

Expression and localization of MYH11 in mouse tissues, oocytes and preimplantation embryos

  • Junbo Fang
  • Diefang Meng
  • Jianchao Huo
  • Yanyan Zhang
  • Hui PengEmail author
Original Article


Myosin heavy chain 11 (MYH11), a member of the myosin family, is an actin-based cytoskeletal motor protein. Limited previous studies have revealed that the Myh11 gene is expressed in cumulus cells, cumulus-oocyte complexes, and ovarian theca cells. However, its expression profiles in mouse tissues, oocytes and preimplantation embryos are largely unknown. Here, we found that the MYH11 protein was expressed in multiple mouse tissues. In the ovary, the MYH11 protein was highly expressed in oocytes and smooth muscle fiber cells. After ovulation and fertilization, Myh11 mRNA expression had sharply declined in zygotes and could not be detected from 8-cell to blastocyst stages, while the protein persisted until the blastocyst stage. Furthermore, the MYH11 protein was localized in the cytoplasm of oocytes and preimplantation embryos. These results provide the first evidence that MYH11 is enriched in oocytes and early embryos and might play a key role in oogenesis and early embryogenesis of mice.


Myosin heavy chain 11 Expression profile Oocyte Preimplantation embryo Mouse 



The authors thank Professor Wenchang Zhang for helpful discussions. This work was supported by grants from the National Natural Science Foundation of China (grant number 31672415), Fujian Provincial Natural Science Foundation (grant number 2018 J01594), and FAFU Program for Distinguished Young Scholars (grant number XJQ201509).

Compliance with ethical standards

Competing interests

The authors have declared that no competing interests exist.


  1. An Q, Peng W, Cheng Y, Lu Z, Zhou C, Zhang Y, Su J (2019) Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos. J Cell Physiol 234:1–12. CrossRefGoogle Scholar
  2. Babu GJ, Warshaw DM, Periasamy M (2000) Smooth muscle myosin heavy chain isoforms and their role in muscle physiology. Microsc Res Techn 50:532–540.<532::AID-JEMT10>3.0.CO;2-E CrossRefGoogle Scholar
  3. Babu GJ, Pyne GJ, Zhou Y et al (2004) Isoform switching from SM-B to SM-A myosin results in decreased contractility and altered expression of thin filament regulatory proteins. Am J Physiol Cell Physiol 287:C723–C729. CrossRefPubMedGoogle Scholar
  4. Barfeld SJ, East P, Zuber V, Mills IG (2014) Meta-analysis of prostate cancer gene expression data identifies a novel discriminatory signature enriched for glycosylating enzymes. BMC Med Genom 7:513. CrossRefGoogle Scholar
  5. Deng Z, Liu P, Marlton P et al (1993) Smooth muscle myosin heavy chain locus (MYH11) maps to 16p13.13-p13.12 and establishes a new region of conserved synteny between human 16p and mouse 16. Genomics 18:156–159. CrossRefPubMedGoogle Scholar
  6. Foth BJ, Goedecke MC, Soldati D (2006) New insights into myosin evolution and classification. Proc Natl Acad Sci U S A 103:3681–3686. CrossRefPubMedPubMedCentralGoogle Scholar
  7. García-Láez V, Beltrán D, De los Santos JM, Esteban FJ, Martínez-Conejero JA, De los Santos MJ (2011) Identification of gene expression of MYH11 and PF4V1 in cumulus cells (CC) after ovarian stimulation by microarrays technology: possible implications on oocyte maturation. Fertil Steril 96:S240. CrossRefGoogle Scholar
  8. Hao Y, Cui Y, Gu X (2016) Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing. Sci Rep 6:27507. CrossRefPubMedPubMedCentralGoogle Scholar
  9. Itami N, Kawahara-Miki R, Kawana H, Endo M, Kuwayama T, Iwata H (2014) Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles. J Assist Repr Genet 31:1079–1088. CrossRefGoogle Scholar
  10. Korn ED (2000) Coevolution of head, neck, and tail domains of myosin heavy chains. Proc Natl Acad Sci U S A 97:12559–12564. CrossRefPubMedPubMedCentralGoogle Scholar
  11. Landreh L, Stukenborg JB, Soder O, Svechnikov K (2013) Phenotype and steroidogenic potential of PDGFRα-positive rat neonatal peritubular cells. Mol Cell Endocrinol 372:96–104. CrossRefPubMedGoogle Scholar
  12. Li L, Baibakov B, Dean J (2008) A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell 15:416–425. CrossRefPubMedPubMedCentralGoogle Scholar
  13. Masters TA, Kendrick-Jones J, Buss F (2017) Myosins: domain organisation, motor properties, physiological roles and cellular functions. In: Jockusch BM (ed) The actin cytoskeleton. Springer International Publishing, Cham, pp 77–122. CrossRefGoogle Scholar
  14. McGuire MM, Bowden W, Engel NJ, Ahn HW, Kovanci E, Rajkovic A (2011) Genomic analysis using high-resolution single-nucleotide polymorphism arrays reveals novel microdeletions associated with premature ovarian failure. Fertil Steril 95:1595–1600. CrossRefPubMedPubMedCentralGoogle Scholar
  15. Miano JM, Cserjesi P, Ligon KL, Periasamy M, Olson EN (1994) Smooth muscle myosin heavy chain exclusively marks the smooth muscle lineage during mouse embryogenesis. Circ Res 75:803–812. CrossRefPubMedGoogle Scholar
  16. Okumu LA, Bruinton S, Braden TD, Simon L, Goyal HO (2012) Estrogen-induced maldevelopment of the penis involves down-regulation of myosin heavy chain 11 (MYH11) expression, a biomarker for smooth muscle cell differentiation. Biol Reprod 87:109. CrossRefPubMedPubMedCentralGoogle Scholar
  17. Pannu H, Tran-Fadulu V, Papke CL et al (2007) MYH11 mutations result in a distinct vascular pathology driven by insulin-like growth factor 1 and angiotensin II. Hum Mol Genet 16:2453–2462. CrossRefPubMedPubMedCentralGoogle Scholar
  18. Peng H, Chang B, Lu C et al (2012) Nlrp2, a maternal effect gene required for early embryonic development in the mouse. PLoS One 7:e30344. CrossRefPubMedPubMedCentralGoogle Scholar
  19. Peng H, Lin X, Liu F, Wang C, Zhang W (2015) NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse. J Reprod Develop 61:559–564. CrossRefGoogle Scholar
  20. Roussis IM, Guille M, Myers FA, Scarlett GP (2016) RNA whole-mount in situ hybridisation proximity ligation assay (rISH-PLA), an assay for detecting RNA-protein complexes in intact cells. PLoS One 11:e0147967. CrossRefPubMedPubMedCentralGoogle Scholar
  21. Schutz LF, Hurst RE, Schreiber NB, Spicer LJ (2018) Transcriptome profiling of bovine ovarian theca cells treated with fibroblast growth factor 9. Domest Anim Endocrin 63:48–58. CrossRefGoogle Scholar
  22. Tong ZB, Gold L, De Pol A et al (2004) Developmental expression and subcellular localization of mouse MATER, an oocyte-specific protein essential for early development. Endocrinology 145:1427–1434. CrossRefPubMedGoogle Scholar
  23. Wen Q, Wang Y, Tang J, Cheng CY, Liu YX (2016) Sertoli cell Wt1 regulates peritubular Myoid cell and fetal Leydig cell differentiation during fetal testis development. PLoS One 11:e0167920. CrossRefPubMedPubMedCentralGoogle Scholar
  24. Yan J, Zhang L, Xu J et al (2014) Smad4 regulates ureteral smooth muscle cell differentiation during mouse embryogenesis. PLoS One 9:e104503. CrossRefPubMedPubMedCentralGoogle Scholar
  25. Yang F, Wei Q, Adelstein RS, Wang PJ (2012) Non-muscle myosin IIB is essential for cytokinesis during male meiotic cell divisions. Dev Biol 369:356–361. CrossRefPubMedPubMedCentralGoogle Scholar
  26. Zhang W, Cheng Z, Qu X, Dai H, Ke X, Chen Z (2014) Overexpression of myosin is associated with the development of uterine myoma. J Obstet Gynaecol Re 40:2051–2057. CrossRefGoogle Scholar

Copyright information

© Institute of Zoology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Junbo Fang
    • 1
  • Diefang Meng
    • 1
  • Jianchao Huo
    • 1
  • Yanyan Zhang
    • 1
  • Hui Peng
    • 1
    • 2
    Email author
  1. 1.College of Animal ScienceFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  2. 2.University Key Lab for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian ProvinceFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China

Personalised recommendations