, Volume 74, Issue 12, pp 1579–1590 | Cite as

Lipid production in Dunaliella salina under autotrophic, heterotrophic, and mixotrophic conditions

  • Zohreh Zare Chavoshi
  • Mansour ShariatiEmail author
Original Article


The effects of autotrophic, heterotrophic, and mixotrophic conditions were examined on micro green alga Dunaliella salina (UTEX 200) in terms of cell growth, total chlorophyll concentration, and lipid production. Results revealed a minimum growth rate of microalgae in the heterotrophic condition which is not recommended for D. salina. When the cells were cultured in mixotrophic conditions containing different concentrations of acetate or glucose, in 100 mM acetate or 60 mM glucose, the highest levels of growth rate and lipid production were obtained compared with autotrophic culture; however, the chlorophyll concentration decreased. Moreover, a significant correlation between ROS production and lipid synthesis was observed. When the combined acetate and glucose was added to the culture, no increase in growth rate and lipid synthesis was observed. This might be due to substrate inhibition. In the presence of glucose, the acidification of culture and bleaching occurred. Results indicated that lipid production is not affected by acidified conditions. In general, our results confirmed that mixotrophic culture with 100 mM acetate or 60 mM glucose is the most appropriate culture for D. salina concerning high lipid production as well as high growth rate in the shortest period of time.


Autotrophic Dunaliella salina Heterotrophic Mixotrophic 



We would like to thank the Graduate School of the University of Isfahan for providing research facilities for this study. Authors acknowledge Plant Antioxidant Center of Excellence (PACE), University of Isfahan.

Compliance with ethical standards

The authors declare that they have no conflict of interest.

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of BiologyUniversity of IsfahanIsfahanIran

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