Genome wide characterization, evolution and expression analysis of FBA gene family under salt stress in Gossypium species
Abiotic stress is an important bottleneck in crop yield around the world. The main purpose of this study is to describe the function and expression patterns of Fructose-1, 6-bisphosphate Aldolase (FBA) gene family in Gossypium species under salt stress. FBA genes are implicated in plant growth and development as well as salt stress tolerance. In this study, 19 FBA genes were found in G. hirsutum, while 9 genes each in case of G. arboreum and G. raimondii. Based on protein domains and phylogenetic analysis, FBA genes were categorized into two classes I and II. Gene structure analysis and motifs division of FBA genes in three Gossypium species showed that numerous genes of FBA have unequal distribution of introns, suggesting new functions in the gene family. Combined syntenic blocks of FBA genes revealed 31duplicated genes among the three Gossypium species. Three kinds of duplications (segmental, tandem and dispersed) were observed with most of the FBA genes duplicated in segmental pattern and remaineds conserved, which could be a possible basis of expansion of FBA genes in cotton (Gossypium). Gene ontology results showed that FBA genes were involved in a couple of mechanisms such as vacuole compartmentalization, glycolysis process and zinc ion binding. The expression analysis depicted that most of class I genes were upregulated in salt tolerant species, G. darwinii as compared to salt susceptible species G. hirsutum. These finding shows comprehensive information on structure and promising function of FBA gene family in Gossypium plant under salt stress.
KeywordsG. darwinii FBA gene family G. hirsutum Gene expression Salt stress
Circular Genome Data Visualization
Gene Structure Display Server
Fructose-1, 6-bisphosphate aldolase
Hidden Markov Model
Million years ago
We express gratitude to all of our team and lab fellows who have helped us in our research experiment
M.S., K.W. and F.L. designed this research experiment. M.S. organized experiment, data collection and analysis of results. M.S. and A.D. created and revised a manuscript. A.D., F.L., X.C., X.W., Y.X., Y.H., M.Y., Z.Z. and M.K. reviewed the manuscript; finally, all authors examined and accepted this manuscript for submission.
The research work of this project was supported by National key research and development plan (2016YFD0100306, 2017YFD0101601) and National Natural Science Foundation of China (31530053, 31671745).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no competing interests.
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