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Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol

  • Zhou-Xin Yang
  • Dong-Yang GuoEmail author
  • Ling-Zhi Shen
  • Gen-Xiang Mao
  • Ji-Huan Dai
  • Sha-Sha Chen
  • Jing YanEmail author
Original Article


Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases.


Panaxadiol Poly (I:C) Inflammatory Transcriptome 



Double-stranded ribonucleic acid


Toll-like receptors 3


Tumor necrosis factor alpha




Polymerase chain reaction


Deoxyribonucleic acid


Chemokine (C-X-C motif) ligand 2


Colony stimulating factor 3


Prostaglandin endoperoxide synthase 2


Aconitate decarboxylase 1


Leukemia inhibitory factor


Interleukin 1 beta


Glyceraldehyde-3-phosphate dehydrogenase


Kyoto Encyclopedia of Genes and Genomes


Gene Ontology

RPMI 1640

Roswell Park Memorial Institute 1640


Enzyme linked immunosorbent assay


Standard deviation


Graft-versus-host disease



This work was supported by the National Natural Science Foundation of China (81772051, 81501113, 81801902, 81801903), Health Bureau of Zhejiang Province (2017KY001), Chinese traditional medicine science and technology projects of Zhejiang Province (2017ZA001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.


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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Zhou-Xin Yang
    • 1
  • Dong-Yang Guo
    • 2
    Email author
  • Ling-Zhi Shen
    • 3
  • Gen-Xiang Mao
    • 2
  • Ji-Huan Dai
    • 2
  • Sha-Sha Chen
    • 2
  • Jing Yan
    • 2
    Email author
  1. 1.Department of Second Clinical Medical CollegeZhejiang Chinese Medical UniversityHangzhouChina
  2. 2.Department of Critical Care MedicineZhejiang HospitalHangzhouChina
  3. 3.Faculty of Clinical MedicineWenzhou Medical UniversityWenzhouChina

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