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Application of the alizarin red S mass marking technique and its detection in stocked asp (Leuciscus aspius)

  • Karel Halačka
  • Jan Mareš
  • Lukáš Vetešník
  • Petr BlabolilEmail author
Short Communication


Alizarin Red S marking and detection was tested on asp (Leuciscus aspius Linnaeus, 1758) in an experimental pool followed by a mark-recapture experiment in the field. Placing the asp in a 0.15 g L−1 solution for one hour produced marks that were detectable for at least one year. In order to detect the fluorescent marks, novel equipment was developed comprising a battery-powered laser source (<100 mW laser pointer, wavelength 532 nm) and protective filtration goggles (filtering wavelength 190–540 nm). The detection equipment and procedure proved satisfactory for marking large numbers of fish and for detection in the field. It allowed repeat observations of the same fish and examination of fin rays (eventually fin clips), which are usually too large to examine under a standard microscope. This method is highly recommended for future studies.


Aspius aspius Fin rays Fluorescence Green laser Non-invasive detection 



This study was supported through project QJ1620240 “Application of “top-down” biomanipulation to reduce eutrophication caused by agriculture in reservoirs“. Thanks are given to Dr. Kevin Roche for language correction.

Compliance with ethical standards

Ethical approval

All applicable international, national and institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors declare that no competing interests exist.


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Copyright information

© Institute of Zoology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Karel Halačka
    • 1
  • Jan Mareš
    • 2
  • Lukáš Vetešník
    • 1
  • Petr Blabolil
    • 3
    Email author
  1. 1.Institute of Vertebrate Biology of the Academy of Sciences of the Czech RepublicBrnoCzech Republic
  2. 2.Department of Fisheries and HydrobiologyMendel University in BrnoBrnoCzech Republic
  3. 3.Institute of HydrobiologyBiology Centre of the Czech Academy of SciencesČeské BudějoviceCzech Republic

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