A comparative study of Trypanosoma sp. (Protozoa: Kinetoplastida) parasitizing four species of loricarides in the Brazilian Amazon
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Trypanosomes are flagellate hemoparasites that infect a wide variety of vertebrates, with neotropical freshwater fish of the Loricariidae family among their known hosts. The present study describes the morphometry of three morphotypes of Trypanosoma sp. parasites of four species of loricariids in the Brazilian Amazon. Between April and May 2018, 28 specimens of Pterygoplichthys pardalis were captured in Pacoval Lake and one specimen of Hypostomus sp., one specimen of Squaliforma sp. and one representative of Lamontichthys cf. stibaros were collected from the mouth of the Tapajós River, in an area known as Enseada Grande, located in the municipal region of Santarém, in the west of the state of Pará, Brazil. A blood sample was collected by cardiac puncture and smears were prepared. The trypanosomes were photographed to allow morphometry to be carried out. The specimens of Squaliforma sp., Lamontichthys cf. stibaros and Hypostomus sp. exhibited infection with trypanosomatids whereas of the 28 specimens of P. pardalis, only five were infected by Trypanosoma sp., representing a prevalence of 17.86%, with a mean infection intensity of 11 × 104 parasites/mL and an average abundance of 0.37. The presence of three morphological types of Trypanosoma sp. infecting the Loricariids was identified. Morphotype A was identified infecting P. pardalis and L. cf. stibaros, morphotype B infected Squaliforma sp. and Hypostomus sp. and morphotype C infected only L. cf. stibaros. Mixed infection by morphotypes A and C was observed in L. cf. stibaros.
KeywordsHemoparasites Fish Brazilian Amazon
The authors would like to thank the Coordination for the Improvement of Higher Level Personnel (CAPES) for the scholarship of Darlison C. Souza.
Compliance with ethical standards
The authors are fully committed to the COPE guidelines (Committee On Publication Ethics) and declare that all institutional and national guidelines for the care and use of animals were followed. The method by the was approved by the Ethics Committee on the Use of Animals of the Universidade Federal do Oeste do Pará under certificate number 0420180027. Was also submitted to the national genetic heritage management system and associated traditional knowledge, as summarized below, was registered in SisGen, in compliance with the provisions of Law 13,123/2015 and certificate number A099911.
Conflict of interest
The authors declare that they have no conflict of interest.
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