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Biologia

, Volume 74, Issue 8, pp 929–939 | Cite as

Barley and spelt differ in leaf silicon content and other leaf traits

  • Mateja GrašičEmail author
  • Barbara Škoda
  • Aleksandra Golob
  • Katarina Vogel-Mikuš
  • Alenka Gaberščik
Original Article
  • 45 Downloads

Abstract

Silicon is considered to be a beneficial element for plants, improving their potential to overcome various stress conditions. Accumulation of silicon differs across different plant species and during plant development, leading to differences in their sensitivity to environmental constraints. We studied the leaf contents of silicon and some other elements for barley (Hordeum vulgare L.) and spelt (Triticum spelta L.) in their vegetative and reproductive stages, while also monitoring the different morphological, biochemical, and optical leaf traits. For barley, the leaf silicon and calcium contents were 1.6% and 1.1%, respectively, and for spelt, they were 1.4% and 0.6%, respectively. There were considerable morphological differences between these two species, including significantly higher prickle hair density in barley, which was positively related to leaf contents of phytoliths, silicon, and calcium. The reflectance of the barley leaves was significantly (p ≤ 0.05) positively related to leaf phytolith and silicon contents throughout the whole spectrum, while light transmittance was significantly (p ≤ 0.05) negatively related to leaf phytolith and silicon contents in the visible and near infrared regions. For spelt, there were no such correlations. Barley showed a significant increase in total and phytolith-bound silicon in the leaves from the vegetative to reproductive stage, which was not the case in spelt. Differences between barley and spelt were also observed in stomata density and length, which would also affect water management in these plants, and thus also their uptake of silicon and calcium.

Keywords

Hordeum vulgare L. Triticum spelta L. Vegetative stage Reproductive stage Silicon accumulation 

Notes

Acknowledgements

The authors are grateful to Christopher Berrie for revision of the English writing. The authors acknowledge financial support from the Slovenian Research Agency through core research funding for the programme Plant Biology (P1-0212), and the project Young Researchers (39096).

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

References

  1. Baltzer JL, Thomas SC (2005) Leaf optical responses to light and soil nutrient availability in temperate deciduous trees. Am J Bot 92:214–223.  https://doi.org/10.3732/ajb.92.2.214 CrossRefGoogle Scholar
  2. Beavers AH, Jones RL (1963) Some mineralogical and chemical properties of plant opal. Soil Sci 96:375–379CrossRefGoogle Scholar
  3. Bonafaccia G, Galli V, Francisci R, Mair V, Skrabanja V, Kreft I (2000) Characteristics of spelt wheat products and nutritional value of spelt wheat-based bread. Food Chem 68:437–441.  https://doi.org/10.1016/S0308-8146(99)00215-0 CrossRefGoogle Scholar
  4. Bonafaccia G, Merendino N, Bonafaccia F, Molinari R, Galli V, Pravst I, Škrabanja V, Luthar Z, Golob A, Germ M (2016) Concentration of proteins, β-glucans, total phenols and antioxidant capacity of Slovenian samples of barley. Folia Biologica et Geologica 57:11–18.  https://doi.org/10.3986/fbg0015 CrossRefGoogle Scholar
  5. Cakmak I (2005) The role of potassium in alleviating detrimental effects of abiotic stresses in plants. J Plant Nutr Soil Sci 168:521–530.  https://doi.org/10.1002/jpln.200420485 CrossRefGoogle Scholar
  6. Caldwell MM (1968) Solar ultraviolet radiation as an ecological factor for alpine plants. Ecol Monogr 38:243–268.  https://doi.org/10.2307/1942430 CrossRefGoogle Scholar
  7. Campbell KG (1997) Spelt: agronomy, genetics, and breeding. In: Janick J (ed) Plant breeding reviews, vol 15. John Wiley and Sons, Inc, New York, pp 187–213.  https://doi.org/10.1002/9780470650097.ch6 Google Scholar
  8. Carter GA (1991) Primary and secondary effects of water content on the spectral reflectance of leaves. Am J Bot 78:916–924CrossRefGoogle Scholar
  9. Castro KL, Sanchez-Azofeifa GA (2008) Changes in spectral properties, chlorophyll content and internal mesophyll structure of senescing Populus balsamifera and Populus tremuloides leaves. Sensors 8:51–69.  https://doi.org/10.3390/s8010051 CrossRefGoogle Scholar
  10. Cooke J, Leishman M (2011) Silicon concentration and leaf longevity: is silicon a player in the leaf dry mass spectrum? Funct Ecol 25:1181–1188.  https://doi.org/10.1111/j.1365-2435.2011.01880.x CrossRefGoogle Scholar
  11. Demmig-Adams B, Adams WW (2002) Antioxidants in photosynthesis and human nutrition. Science 298:2149–2153.  https://doi.org/10.1126/science.1078002 CrossRefGoogle Scholar
  12. Dietrich D, Hinke S, Baumann W, Fehlhaber R, Bäucker E, Rühle G, Wienhaus O, Marx G (2003) Silica accumulation in Triticum aestivum L. and Dactylis glomerata L. Anal Bioanal Chem 376:399–404.  https://doi.org/10.1007/s00216-003-1847-8 CrossRefGoogle Scholar
  13. Drumm H, Mohr H (1978) The mode of interaction between blue (UV) light photoreceptor and phytochrome in anthocyanin formation of the Sorghum seedling. Photochem Photobiol 27:241–248.  https://doi.org/10.1111/j.1751-1097.1978.tb07595.x CrossRefGoogle Scholar
  14. Faisal S, Callis KL, Slot M, Kitajima K (2012) Transpiration-dependent passive silica accumulation in cucumber (Cucumis sativus) under varying soil silicon availability. Botany 90:1058–1064.  https://doi.org/10.1139/B2012-072 CrossRefGoogle Scholar
  15. Gal A, Brumfeld V, Weiner S, Addadi L, Oron D (2012) Certain biominerals in leaves function as light scatterers. Adv Opt Mater 24:77–83.  https://doi.org/10.1002/adma.201104548 Google Scholar
  16. Gawlik-Dziki U, Świeca M, Dziki D (2012) Comparison of phenolic acids profile and antioxidant potential of six varieties of spelt (Triticum spelta L.). J Agric Food Chem 60:4603–4612.  https://doi.org/10.1021/jf3011239 CrossRefGoogle Scholar
  17. Gitelson AA, Zur Y, Chivkunova OB, Merzlyak MN (2002) Assessing carotenoid content in plant leaves with reflectance spectroscopy. Photochem Photobiol 75:272–281.  https://doi.org/10.1562/0031-8655(2002)0750272ACCIPL2.0.CO2 CrossRefGoogle Scholar
  18. González A, Martín I, Ayerbe L (1999) Barley yield in water-stress conditions: the influence of precocity osmotic adjustment and stomatal conductance. Field Crop Res 62:23–34.  https://doi.org/10.1016/S0378-4290(99)00002-7 CrossRefGoogle Scholar
  19. Gould KS (2004) Nature’s Swiss army knife: the diverse protective roles of anthocyanins in leaves. J Biomed Biotechnol 5:314–320.  https://doi.org/10.1155/S1110724304406147 CrossRefGoogle Scholar
  20. Hodson MJ, Sangster AG (1988) Observations on the distribution of mineral elements in the leaf of wheat (Triticum aestivum L.), with particular reference to silicon. Ann Bot 62:463–471.  https://doi.org/10.2307/2656798 CrossRefGoogle Scholar
  21. Hosseini MM, Shao Y, Whalen JK (2011) Biocement production from silicon-rich plant residues: perspectives and future potential in Canada. Biosyst Eng 110:351–362.  https://doi.org/10.1016/j.biosystemseng.2011.09.010 CrossRefGoogle Scholar
  22. Huete AR (2004) Remote sensing for environmental monitoring. In: Janick FA, Pepper IL, Brusseau ML (eds) Environmental monitoring and characterization, 1st edn. Elsevier Academic Press, Burlington, pp 183–206.  https://doi.org/10.1016/B978-012064477-3/50013-8 CrossRefGoogle Scholar
  23. Jones LHP, Milne AA (1963) Studies of silica in the oat plant I. Chemical and physical properties of the silica. Plant Soil 18:207–220CrossRefGoogle Scholar
  24. Kaufman PB, Dayanandan P, Takeoka Y, Bigelow JD, Jones JD, Iler R (1981) Silica in shoots of higher plants. In: Simpson TL, Volcani BE (eds) Silicon and siliceous structures in biological systems. Springer, New York, pp 409–449.  https://doi.org/10.1007/978-1-4612-5944-2_15 CrossRefGoogle Scholar
  25. Klančnik K, Vogel-Mikuš K, Gaberščik A (2014a) Silicified structures affect leaf optical properties in grasses and sedge. J Photochem Photobiol B Biol 130:1–10.  https://doi.org/10.1016/j.jphotobiol.2013.10.011 CrossRefGoogle Scholar
  26. Klančnik K, Vogel-Mikuš K, Kelemen M, Vavpetič P, Pelicon P, Kump P, Jezeršek D, Gianoncelli A, Gaberščik A (2014b) Leaf optical properties are affected by the location and type of deposited biominerals. J Photochem Photobiol B Biol 140:276–285.  https://doi.org/10.1016/j.jphotobiol.2014.08.010 CrossRefGoogle Scholar
  27. Klančnik K, Zelnik I, Gnezda P, Gaberščik A (2015) Do reflectance spectra of different plant stands in wetland indicate species properties? In: Vymazal J (ed) The role of natural and constructed wetlands in nutrient cycling and retention on the landscape. Springer, Cham, pp 73–86.  https://doi.org/10.1007/978-3-319-08177-9_6 Google Scholar
  28. Kump P, Nečemer M, Rupnik Z, Pelicon P, Ponikvar D, Vogel-Mikuš K, Regvar M, Pongrac P (2011) Improvement of the XRF quantification and enhancement of the combined applications by EDXRF and micro-PIXE. In: Integration of nuclear spectrometry methods as a new approach to material research. IAEA, Vienna, pp 101–109Google Scholar
  29. Larcher W (2003) Physiological plant ecology: ecophysiology and stress physiology of functional groups, 4th edn. Springer, BerlinCrossRefGoogle Scholar
  30. Levizou E, Drilias P, Psaras GK, Manetas Y (2005) Nondestructive assessment of leaf chemistry and physiology through spectral reflectance measurements may be misleading when changes in trichome density co-occur. New Phytol 165:463–472.  https://doi.org/10.1111/j.1469-8137.2004.01250.x CrossRefGoogle Scholar
  31. Liakatas A, Proutsos N, Alexandris S (2002) Optical properties affecting the radiant energy of an oak forest. Meteorol Appl 9:433–436.  https://doi.org/10.1017/S135048270200405X CrossRefGoogle Scholar
  32. Liang Y, Sun W, Zhu YG, Christie P (2007) Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environ Pollut 147:422–428.  https://doi.org/10.1016/j.envpol.2006.06.008 CrossRefGoogle Scholar
  33. Lichtenthaler HK, Buschmann C (2001a) Extraction of photosynthetic tissues: chlorophylls and carotenoids. Curr Protocol Food Anal Chem 1:165–170.  https://doi.org/10.1002/0471709085.ch21 Google Scholar
  34. Lichtenthaler HK, Buschmann C (2001b) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Curr Protocol Food Anal Chem 1:171–178.  https://doi.org/10.1002/0471709085.ch21 Google Scholar
  35. Lu H, Zhang J, Wu N, Liu KB, Xu D, Li Q (2009) Phytoliths analysis for the discrimination of foxtail millet (Setaria italica) and common millet (Panicum miliaceum). PLoS One 4:e4448.  https://doi.org/10.1371/journal.pone.0004448 CrossRefGoogle Scholar
  36. Ma JF (1990) Studies on beneficial effects of silicon on Rice plants. Kyoto University, DissertationGoogle Scholar
  37. Ma JF, Takahashi E (2002) Soil, fertilizer, and plant silicon research in Japan, 1st edn. Elsevier, AmsterdamGoogle Scholar
  38. Markovich O, Kumar S, Cohen D, Addadi S, Fridman E, Elbaum R (2015) Silicification in leaves of sorghum mutant with low silicon accumulation. Silicon.  https://doi.org/10.1007/s12633-015-9348-x
  39. Mathur S, Jajoo A (2014) Alterations in photochemical efficiency of photosystem II in wheat plant on hot summer day. Physiol Mol Biol Plants 20:527–531.  https://doi.org/10.1007/s12298-014-0249-z CrossRefGoogle Scholar
  40. McNaughton SJ, Tarrants JL, McNaughton MM, Davis RD (1985) Silica as a defense against herbivory and a growth promotor in African grasses. Ecology 66:528–535.  https://doi.org/10.2307/1940401 CrossRefGoogle Scholar
  41. Meier U (2001) Growth stages of mono-and dicotyledonous plants. Federal Biological Research Centre for Agriculture and Forestry. https://www.politicheagricole.it/flex/AppData/WebLive/Agrometeo/MIEPFY800/BBCHengl2001.pdf. Accessed 12 December 2018
  42. Meunier JD, Colin F, Alarcon C (1999) Biogenic silica storage in soils. Geology 27:835–838.  https://doi.org/10.1130/0091-7613(1999)027<0835:BSSIS>2.3.CO;2 CrossRefGoogle Scholar
  43. Miyake Y, Takahashi E (1978) Silicon deficiency of tomato plant. Soil Sci Plant Nutr 24:175–189.  https://doi.org/10.1080/00380768.1978.10433094 CrossRefGoogle Scholar
  44. Miyake Y, Takahashi E (1985) Effect of silicon on the growth of soybean plants in solution culture. Soil Sci Plant Nutr 31:625–636.  https://doi.org/10.1080/00380768.1986.10557510 CrossRefGoogle Scholar
  45. Mooney HA, Ehleringer J, Björkman O (1977) The energy balance of leaves of the evergreen desert shrub Atriplex hymenelytra. Oecologia 29:301–310.  https://doi.org/10.1007/BF00345804 CrossRefGoogle Scholar
  46. Morikawa CK, Saigusa M (2004) Mineral composition and accumulation of silicon in tissues of blueberry (Vaccinum corymbosus cv. Bluecrop) cuttings. Plant Soil 258:1–8.  https://doi.org/10.1023/B:PLSO.0000016489.69114.55 CrossRefGoogle Scholar
  47. Motomura H, Fujii T, Suzuki M (2006) Silica deposition in abaxial epidermis before the opening of leaf blades of Pleioblastus chino (Poaceae, Bambusoideae). Ann Bot 97:513–519.  https://doi.org/10.1093/aob/mcl014 CrossRefGoogle Scholar
  48. Nečemer M, Kump P, Ščančar J, Jaćimović R, Simčič J, Pelicon P, Budnar M, Jeran Z, Pongrac P, Regvar M, Vogel-Mikuš K (2008) Application of X-ray fluorescence analytical techniques in phytoremediation and plant biology studies. Spectrochim Acta B At Spectrosc 63:1240–1247.  https://doi.org/10.1016/j.sab.2008.07.006 CrossRefGoogle Scholar
  49. Nikolic M, Nikolic N, Liang Y, Kirkby EA, Römheld V (2007) Germanium-68 as an adequate tracer for silicon transport in plants. Characterization of silicon uptake in different crop species. Plant Physiol 143:495–503.  https://doi.org/10.1104/pp.106.090845 CrossRefGoogle Scholar
  50. Out WA, Madella M (2016) Morphometric distinction between bilobate phytoliths from Panicum miliaceum and Setaria italica leaves. Archaeol Anthropol Sci 8:505–521.  https://doi.org/10.1007/s12520-015-0235-6 CrossRefGoogle Scholar
  51. Perry CC, Williams RJP, Fry SC (1987) Cell wall biosynthesis during silicification of grass hairs. J Plant Physiol 126:437–448.  https://doi.org/10.1016/S0176-1617(87)80028-7 CrossRefGoogle Scholar
  52. Piperno DR (2006) Phytoliths: a comprehensive guide for archaeologists and Paleoecologists. AltaMira Press, OxfordGoogle Scholar
  53. Prychid CJ, Rudall PJ, Gregory M (2003) Systematics and biology of silica bodies in monocotyledons. Bot Rev 69:377–440. https://doi.org/10.1663/0006-8101(2004)069[0377:SABOSB]2.0.CO;2Google Scholar
  54. Raven JA (1983) The transport and function of silicon in plants. Biol Rev 58:179–207.  https://doi.org/10.1111/j.1469-185X.1983.tb00385.x CrossRefGoogle Scholar
  55. Rovner I (1971) Potential of opal phytoliths for use in paleoecological reconstruction. Quat Res 1:343–359.  https://doi.org/10.1016/0033-5894(71)90070-6 CrossRefGoogle Scholar
  56. Rozema J, Björn LO, Bornman JF, Gaberščik A, Hader DP, Trošt T, Germ M, Klisch M, Gröniger A, Sinha RP, Lebert M, He YY, Buffoni-Hall R, de Bakker NVJ, van de Staaij J, Meijkamp BB (2002) The role of UV-B radiation in aquatic and terrestrial ecosystems—an experimental and functional analysis of the evolution of UV-absorbing compounds. J Photochem Photobiol B Biol 66:2–12.  https://doi.org/10.1016/S1011-1344(01)00269-X CrossRefGoogle Scholar
  57. Sánchez-Díaz M, García JL, Antolín MC, Araus JL (2002) Effects of soil drought and atmospheric humidity on yield, gas exchange, and stable carbon isotope composition of barley. Photosynthetica 40:415–421.  https://doi.org/10.1023/A:1022683210334 CrossRefGoogle Scholar
  58. Sangster AG, Wynn Parry D (1969) Some factors in relation to bulliform cell silicification in the grass leaf. Ann Bot 33:315–323.  https://doi.org/10.1093/oxfordjournals.aob.a084285 CrossRefGoogle Scholar
  59. Schoelynck J, Bal K, Backx H, Okruszko T, Meire P, Struyf E (2010) Silica uptake in aquatic and wetland macrophytes: a strategic choice between silica, lignin and cellulose? New Phytol 186:385–391.  https://doi.org/10.1111/j.1469-8137.2009.03176.x CrossRefGoogle Scholar
  60. Schreiber U, Kühl M, Klimant I, Reising H (1996) Measurement of chlorophyll fluorescence within leaves using a modified PAM fluorometer with a fiber-optic microprobe. Photosynth Res 47:103–109.  https://doi.org/10.1007/BF00017758 CrossRefGoogle Scholar
  61. Shakhatreh Y, Kafawin O, Ceccarelli S, Saoub H (2001) Selection of barley lines for drought tolerance in low-rainfall areas. J Agron Crop Sci 186:119–127.  https://doi.org/10.1046/j.1439-037x.2001.00459.x CrossRefGoogle Scholar
  62. Slaton MR, Hunt ER Jr, Smith WK (2001) Estimating near-infrared leaf reflectance from leaf structural characteristics. Am J Bot 88:278–284.  https://doi.org/10.2307/2657019 CrossRefGoogle Scholar
  63. Tamai K, Ma JF (2003) Characterization of silicon uptake by rice roots. New Phytol 158:431–436.  https://doi.org/10.1046/j.1469 CrossRefGoogle Scholar
  64. Terashima I, Saeki T (1985) A new model for leaf photosynthesis incorporating the gradients of light environment and of photosynthetic properties of chloroplasts within a leaf. Ann Bot 56:489–499.  https://doi.org/10.1093/oxfordjournals.aob.a087034 CrossRefGoogle Scholar
  65. Tripathi DK, Singh VP, Ahmad P, Chauhan DK, Prasad SM (eds) (2016) Silicon in plants: advances and future prospects. CRC Press, Boca RatonGoogle Scholar
  66. Ullah S, Schlerf M, Skidmore AK, Hecker C (2012) Identifying plant species using mid-wave infrared (2.5-6 μm) and thermal infrared (8-14 μm) emissivity spectra. Remote Sens Environ 118:95–102.  https://doi.org/10.1016/j.rse.2011.11.008 CrossRefGoogle Scholar
  67. Vekemans B, Janssens K, Vincze L, Adams F, Vanespen P (1994) Analysis of X-ray spectra by iterative least squares (AXIL): new developments. X-Ray Spectrom 23:278–285.  https://doi.org/10.1002/xrs.1300230609 CrossRefGoogle Scholar
  68. Vidic NJ, Prus T, Grčman H, Zupan M, Lisec A, Kralj T, Vrščaj B, Rupreht J, Šporar M, Suhadolc M, Mihelič R, Lobnik F (2015) Soils of Slovenia with soil map 1: 250 000. European Commission Joint Research Centre (JRC). http://soil.bf.uni-lj.si/projekti/pdf/atlas_final_2015.pdf. Accessed 12 December 2018
  69. Wang M, Zheng Q, Shen Q, Guo S (2013) The critical role of potassium in plant stress response. Int J Mol Sci 14:7370–7390.  https://doi.org/10.3390/ijms14047370 CrossRefGoogle Scholar
  70. Zhang C, Wang L, Zhang W, Zhang F (2013) Do lignification and silicification of the cell wall precede silicon deposition in the silica cell of the rice (Oryza sativa L.) leaf epidermis? Plant Soil 372:137–149.  https://doi.org/10.1007/s11104-013-1723-z CrossRefGoogle Scholar
  71. Zuk-Golaszewska K, Kurowski T, Załuski D, Sadowska M, Golaszewski J (2015) Physio-agronomic performance of spring cultivars T. aestivum and T. spelta grown in organic farming system. Int J Plant Prod 9:211–236.  https://doi.org/10.22069/IJPP.2015.2063 Google Scholar

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© Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of Biology, Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia

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