, Volume 74, Issue 2, pp 111–117 | Cite as

Sucrose synthesis in Unpollinated ovaries of pomegranate (Punica granatum L.), as well as, in reproductive and vegetative shoot apices

  • Konstantinos MeletisEmail author
  • G. Tsaniklidis
  • I. E. Papadakis
  • S. N. Vemmos
Original Article


Sugar levels, the enzyme activity of UDP-Glucose Pyrophosphorylase (UGPase) and Sucrose Phosphate Synthase (SPS), key enzymes for sucrose synthesis, and the expression of their respective genes were examined in developing vegetative and reproductive shoot apices and in developing unpollinated ovaries of Pomegranate trees in order to detect differences possibly important for fruit production and plant vigor. The level of total sugars was higher in ovaries, followed by reproductive apices and vegetative apices, suggesting higher energy requirements in the reproductive organs. Moreover, the activities of SPS and UGPase were found to be elevated in the vegetative apices, indicating that sucrose synthesis is possibly differently regulated in comparison to the reproductive organs. Our results suggest that the sink power of each organ primarily regulates the levels of both transported (sucrose, mannitol) and non-transported (fructose, glucose) sugars. However, the endogenous production of sucrose may be a regulatory factor contributing to organ development.


APETALA 2 Carbohydrates Enzyme activity Gene expression Sucrose 



The authors would like to thank Prof. G. Aivalakis for his valuable and constructive suggestions during the planning and development of this research work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11756_2018_154_MOESM1_ESM.docx (22 kb)
ESM 1 (DOCX 22 kb)


  1. Ahmed M, Shahid AA, Akhtar S, Latif A, ud Din S, Fanglu M, Rao AQ, Sarwar MB, Husnain T, Xuede W (2018) Sucrose synthase genes: a way forward for cotton fiber improvement. Biologia 73:703–713. CrossRefGoogle Scholar
  2. Babb VM, Haigler CH (2001) Sucrose phosphate synthase activity rises in correlation with high-rate cellulose synthesis in three heterotrophic systems. Plant Physiol 127:1234–1242. CrossRefGoogle Scholar
  3. Bernier G, Périlleux C (2005) A physiological overview of the genetics of flowering time control. Plant Biotechnol J 3:3–16. CrossRefGoogle Scholar
  4. Bernier G, Havelange A, Houssa C, Petitjean A, Lejeune P (1993) Physiological signals that induce flowering. Plant Cell 5:1147–1155. CrossRefGoogle Scholar
  5. Chen A, He S, Li F, Li Z, Ding M, Liu Q, Rong J (2012) Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns. BMC Plant Biol 12:85–102. CrossRefGoogle Scholar
  6. Cheng WH, Chourey PS (1999) Genetic evidence that invertase-mediated release of hexoses is critical for appropriate carbon partitioning and normal seed development in maize. Theor Appl Genet 98:485–495. CrossRefGoogle Scholar
  7. Dai N, Cohen S, Portnoy V, Tzuri G, Harel-Beja R, Pompan-Lotan M, Carmi N, Zhang G, Diber A, Pollock S et al (2011) Metabolism of soluble sugars in developing melon fruit: a global transcriptional view of the metabolic transition to sucrose accumulation. Plant Mol Biol 76:1–18. CrossRefGoogle Scholar
  8. Duclos DV, Björkman T (2008) Meristem identity gene expression during curd proliferation and flower initiation in Brassica oleracea. J Exp Bot 59:421–433. CrossRefGoogle Scholar
  9. Francis D, Halford NG (2006) Nutrient sensing in plant meristems. Plant Mol Biol 60:981–993. CrossRefGoogle Scholar
  10. Garcia-Luis A, Fornes F, Guardiola JL (1995) Leaf carbohydrates and flower formation in citrus. J Am Soc Hortic Sci 120:222–227Google Scholar
  11. Gibson SI (2005) Control of plant development and gene expression by sugar signaling. Curr Op Plant Biol 8:93–102. CrossRefGoogle Scholar
  12. Hanke M, Flachowsky H, Peil A, Hättasch C (2007) No flower no fruit - genetic potentials to trigger flowering in fruit trees. Genes, Genomes, Genomics 1:1–20Google Scholar
  13. Hendriks JHM, Kolbe A, Gibon Y, Stitt M, Geigenberger P (2003) ADP-glucose Pyrophosphorylase is activated by posttranslational redox-modification in response to light and to sugars in leaves of Arabidopsis and other plant species. Plant Physiol 133:838–849. CrossRefGoogle Scholar
  14. Heyer AG, Raap M, Schroeer B, Marty B, Willmitzer L (2004) Cell wall invertase expression at the apical meristem alters floral, architectural, and reproductive traits in Arabidopsis thaliana. Plant 39:161–169. Google Scholar
  15. Holland D, Hatib K, Bar-Ya’akov I (2009) Pomegranate: botany, horticulture, breeding. Horticultural Rev 35:127–191. CrossRefGoogle Scholar
  16. Huber JL, Huber SC, Campbell WH, Redinbaugh MG (1992) Reversible light/dark modulation of spinach leaf nitrate reductase activity involves protein phosphorylation. Arch Biochem Biophys 296:58–65. CrossRefGoogle Scholar
  17. Ito A, Hayama H, Kashimura Y (2002) Sugar metabolism in buds during flower bud formation: a comparison of two Japanese pear [Pyrus pyrifolia (Burm.) Nak.] cultivars possessing different flowering habits. Sci Hort 96:163–175. CrossRefGoogle Scholar
  18. Kalt-Torres W, Kerr PS, Usuda H, Huber SC (1987) Diurnal changes in maize leaf photosynthesis. I. Carbon exchange rate, assimilate export rate, and enzyme activities. Plant Physiol 83:283–288. CrossRefGoogle Scholar
  19. Kleczkowski LA, Geisler M, Ciereszko I, Johansson H (2004) UDP-glucose pyrophosphorylase. An old protein with new tricks. Plant Physiol 134:912–918. CrossRefGoogle Scholar
  20. Koch KE (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Op Plant Biol 7:235–246. CrossRefGoogle Scholar
  21. Lara MEB, Garcia MCG, Fatima T, Ehness R, Lee TK, Proels R et al (2004) Extracellular invertase is an essential component of cytokinin-mediated delay of senescence. Plant Cell 16:1276–1287. CrossRefGoogle Scholar
  22. Lastdrager J, Hanson J, Smeekens S (2014) Sugar signals and the control of plant growth and development. J Exp Bot 65:799–807. CrossRefGoogle Scholar
  23. Lemoine R, La Camera S, Atanassova R, Dédaldéchamp F, Allario T, Pourtau N, Bonnemain JL, Laloi M, Coutos-Thévenot P, Maurousset L et al (2013) Source-to-sink transport of sugar and regulation by environmental factors. Frontiers Plant Sci 4:1–21. CrossRefGoogle Scholar
  24. Martz F, Wilczynska M, Kleczkowski LA (2002) Oligomerization status, with the monomer as active species, defines catalytic efficiency of UDP-glucose pyrophosphorylase. Biochem J 367:295–300. CrossRefGoogle Scholar
  25. Matsoukas IG (2014) Interplay between sugar and hormone signaling pathways modulate floral signal transduction. Plant Genet Genom 218(1–12):5. Google Scholar
  26. Micallef BJ, Haskins KA, Vanderveer PJ, Roh K-S, Shewmaker CK, Sharkey TD (1995) Altered photosynthesis, flowering and fruiting in transgenic tomato plants that have an increased capacity for sucrose synthesis. Planta 196:327–334. CrossRefGoogle Scholar
  27. Ohto M, Onai K, Furukawa Y, Aoki E, Araki T, Nakamura K (2001) Effects of sugar on vegetative development and floral transition in Arabidopsis. Plant Physiol 127:252–261. CrossRefGoogle Scholar
  28. Park JI, Ishimizu T, Suwabe K, Sudo K, Masuko H, Hakozaki H, Nou IS, Suzuki G, Watanabe M (2010) UDP-glucose pyrophosphorylase is rate limiting in vegetative and reproductive phases in Arabidopsis thaliana. Plant Cell Physiol 51:981–996. CrossRefGoogle Scholar
  29. Rabot A, Henry C, Ben Baaziz K, Mortreau E, Azri W, Lothier J et al (2012) Insight into the role of sugars in bud burst under light in the rose. Plant Cell Physiol 53:1068–1082. CrossRefGoogle Scholar
  30. Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell 14:s185–s205. CrossRefGoogle Scholar
  31. Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signaling in plants: conserved and novel mechanisms. Ann Rev Plant Biol 57:675–709. CrossRefGoogle Scholar
  32. Rounis V, Skarmoutsos K, Tsaniklidis G, Nikoloudakis N, Delis C, Karapanos I, Aivalakis G (2015) Seeded and parthenocarpic cherry tomato fruits exhibit similar sucrose, glucose, and fructose levels, despite dissimilarities in UGPase and SPS gene expression and enzyme activity. J Plant Growth Regul 34:47–56. CrossRefGoogle Scholar
  33. Ruan YL (2014) Sucrose metabolism: gateway to diverse carbon use and sugar signaling. Annu Rev Plant Biol 65:33–67. CrossRefGoogle Scholar
  34. Ruan YL, Jin Y, Yang YJ, Li GJ, Boyer JS (2010) Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Mol Plant 3:942–955. CrossRefGoogle Scholar
  35. Sarafi E, Chatzissavvidis C, Therios Ι (2017) Response of two pomegranate (Punica granatum L.) cultivars to six boron concentrations: growth performance, nutrient status, gas exchange parameters, chlorophyll fluorescence, and proline and carbohydrate content. J Plant Nutr 40:983–994. CrossRefGoogle Scholar
  36. Sawicki M, Jacquens L, Baillieul F, Clément C, Vaillant-Gaveau N, Jacquard C (2015) Distinct regulation in inflorescence carbohydrate metabolism according to grapevine cultivars during floral development. Physiol Plant 154:447–467. CrossRefGoogle Scholar
  37. Sergeeva LI, Vreugdenhil D (2002) In situ staining of activities of enzymes involved in carbohydrate metabolism in plant tissues. J Exp Bot 53:361–370. CrossRefGoogle Scholar
  38. Sherson SM, Alford HL, Forbes SM, Wallace G, Smith SM (2003) Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis. J Exp Bot 54:525–531. CrossRefGoogle Scholar
  39. Southerton SG, Strauss SH, Olive MR, Harcourt RL, Decroocq V, Zhu X, Llewellyn DJ, Peacock WJ, Dennis ES (1998) Eucalyptus has a functional equivalent of the Arabidopsis floral meristem identity gene LEAFY. Plant Mol Biol 37:897–910. CrossRefGoogle Scholar
  40. Tognetti JA, Pontis HG, Martinez-Noel GM (2013) Sucrose signaling in plants: a world yet to be explored. Plant Signal Behav e23316(1–10):8. Google Scholar
  41. Vemmos SN (1999) Carbohydrate content of inflorescent buds of defruited and fruiting pistachio (Pistachia vera L.) branches in relation to biennial bearing. Journal Hort Sci Biotech 74:94–100. CrossRefGoogle Scholar
  42. Wang F, Sanz A, Brenner ML, Smith A (1993) Sucrose synthase, starch accumulation, and tomato fruit sink strength. Plant Physiol 101:321–327. CrossRefGoogle Scholar
  43. Weber H, Borisjuk L, Heim U, Wobus U (1997) Sugar import and metabolism during seed development. Trends Plant Sci 2:169–174. CrossRefGoogle Scholar
  44. Wils CR, Kaufmann K (2016) Gene-regulatory networks controlling inflorescence and flower development in Arabidopsis thaliana. Biochim Biophys Acta 1860:95–105. CrossRefGoogle Scholar
  45. Xing LB, Zhang D, Li YM, Shen YW, Zhao CP, Ma JJ et al (2015) Transcription profiles reveal sugar and hormone signaling pathways mediating flower induction in apple (Malus domestica Borkh.). Plant Cell Physiol 56:2052–2068. CrossRefGoogle Scholar
  46. Zanor MI, Osorio S, Nunes-Nesi A, Carrarib F, Lohse M et al (2009) RNA interference of LIN5 in Solanum lycopersicum confirms its role in controlling brix content, uncovers the influence of sugars on the levels of fruit hormones and demonstrates the importance of sucrose cleavage for normal fruit development and fertility. Plant Physiol 150:1204–1218. CrossRefGoogle Scholar
  47. Zhou L, Jang JC, Jones TL, Sheen J (1998) Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant. Proc Natl Acad Sci 95:10294–10299. CrossRefGoogle Scholar

Copyright information

© Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2018

Authors and Affiliations

  • Konstantinos Meletis
    • 1
    Email author
  • G. Tsaniklidis
    • 2
  • I. E. Papadakis
    • 1
  • S. N. Vemmos
    • 1
  1. 1.Laboratory of Pomology, Department of Crop ScienceAgricultural University of AthensAthensGreece
  2. 2.Hellenic Agricultural Organisation “Demeter”Institute of Viticulture, Floriculture and Vegetable CropsHeraklionGreece

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