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Biologia

, Volume 74, Issue 2, pp 111–117 | Cite as

Sucrose synthesis in Unpollinated ovaries of pomegranate (Punica granatum L.), as well as, in reproductive and vegetative shoot apices

  • Konstantinos MeletisEmail author
  • G. Tsaniklidis
  • I. E. Papadakis
  • S. N. Vemmos
Original Article
  • 36 Downloads

Abstract

Sugar levels, the enzyme activity of UDP-Glucose Pyrophosphorylase (UGPase) and Sucrose Phosphate Synthase (SPS), key enzymes for sucrose synthesis, and the expression of their respective genes were examined in developing vegetative and reproductive shoot apices and in developing unpollinated ovaries of Pomegranate trees in order to detect differences possibly important for fruit production and plant vigor. The level of total sugars was higher in ovaries, followed by reproductive apices and vegetative apices, suggesting higher energy requirements in the reproductive organs. Moreover, the activities of SPS and UGPase were found to be elevated in the vegetative apices, indicating that sucrose synthesis is possibly differently regulated in comparison to the reproductive organs. Our results suggest that the sink power of each organ primarily regulates the levels of both transported (sucrose, mannitol) and non-transported (fructose, glucose) sugars. However, the endogenous production of sucrose may be a regulatory factor contributing to organ development.

Keywords

APETALA 2 Carbohydrates Enzyme activity Gene expression Sucrose 

Notes

Acknowledgments

The authors would like to thank Prof. G. Aivalakis for his valuable and constructive suggestions during the planning and development of this research work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11756_2018_154_MOESM1_ESM.docx (22 kb)
ESM 1 (DOCX 22 kb)

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Copyright information

© Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2018

Authors and Affiliations

  • Konstantinos Meletis
    • 1
    Email author
  • G. Tsaniklidis
    • 2
  • I. E. Papadakis
    • 1
  • S. N. Vemmos
    • 1
  1. 1.Laboratory of Pomology, Department of Crop ScienceAgricultural University of AthensAthensGreece
  2. 2.Hellenic Agricultural Organisation “Demeter”Institute of Viticulture, Floriculture and Vegetable CropsHeraklionGreece

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