Genome-wide identification and characterization of cysteine-rich polycomb-like protein (CPP) family genes in cucumber (Cucumis sativus) and their roles in stress responses
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Cysteine-rich polycomb-like protein (CPP) transcription factors are a small gene family involved in the regulation of plant growth, development and stress response. Genome-wide identification and characterization of CPP gene family have been carried out in several plant species. However, no comprehensive phylogenetic analysis or expression profiling of CPP genes in Cucumis sativus has been reported. In this study, we characterized the CPP family genes in C. sativus, and 5 CPP candidate genes were found in cucumber genome. CPP proteins from cucumber and other plant species were classified into two groups, which were further divided into five subgroups based on the phylogenetic distribution. Most CPP genes in the same subgroup shared similar gene structures and conserved motifs. Transcriptome data revealed that CsCPP genes were expressed in leaves, ovaries, flowers, stems, roots, and tendril tissues. qRT-PCR expression analysis showed that many CsCPP genes exhibited different expression patterns in cucumber leaves under abiotic stresses including salt, cold, drought, and ABA. These results demonstrate that cucumber CPP gene family may play critical roles in plant development as well as in the responses to environmental stresses.
KeywordsCucumis sativus Cysteine-rich polycomb-like protein (CPP) Gene family Expression analysis Abiotic stress
Chinese for ‘ugly’
Cysteine-rich polycomb-like protein
Fragments per kb per million mapped read
Grand average of hydropathy index
Hidden Markov model
Quantitative real-time PCR
This work was funded by the Key Project of Youth Science Foundation of Jiangxi Province (20171ACB21025), and the National Natural Science Foundation of China (31460522 and 31660578).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Andersen SU, Algreen-Petersen RG, Hoedl M et al (2007) The conserved cysteine-rich domain of a tesmin/TSO1-like protein binds zinc in vitro and TSO1 is required for both male and female fertility in Arabidopsis thaliana. J Exp Bot 58:3657–3670. https://doi.org/10.1093/jxb/erm215 CrossRefPubMedGoogle Scholar
- Gu Z, Cavalcanti A, Chen FC, Bouman P, Li WH (2002) Extent of gene duplication in the genomes of Drosophila, nematode, and yeast. Mol Biol Evol 19:256–262. https://doi.org/10.1093/oxfordjournals.molbev.a004079 CrossRefPubMedGoogle Scholar
- Song XY, Zhang YY, Wu FC, Zhang L (2016) Genome-wide analysis of the maize (Zea may L.) CPP-like gene family and expression profiling under abiotic stress. Genet Mol Res. https://doi.org/10.4238/gmr.15038023
- Wang W, Sijacic P, Xu P, Lian H, Liu Z (2018) Arabidopsis TSO1 and MYB3R1 form a regulatory module to coordinate cell proliferation with differentiation in shoot and root. Proc Natl Acad Sci U S A. https://doi.org/10.1073/pnas.1715903115
- Zhou Y, Hu L, Wu H, Jiang L, Liu S (2017a) Genome-wide identification and transcriptional expression analysis of cucumber superoxide dismutase (SOD) family in response to various abiotic stresses. Int J Genomics 2017:7243973. https://doi.org/10.1155/2017/7243973 CrossRefPubMedPubMedCentralGoogle Scholar