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Biologia

, Volume 74, Issue 5, pp 477–485 | Cite as

Loop-mediated isothermal amplification combined with PCR for specific identification of injurious mite, Tetranychus urticae (Trombidiformes: Tetranychidae)

  • Samira Sinaie
  • Hussein Sadeghi-NamaghiEmail author
  • Lida Fekrat
Original Article
  • 50 Downloads

Abstract

Identification of spider mites of the genus Tetranychus is strenuous due to their minute size and the restricted number of diagnostic characters. Most species of the Iranian Tetranychus spider mites are morphologically alike, distinguishable only based on male aedeagus; however, most of the intercepted Tetranychus specimens are females, leaving their identification at the genus level. Tetranychus urticae is a highly polyphytophagous pest species commonly found in greenhouses of Iran. As the unambiguous identification of a pest is the first crucial step toward implementing any effective pest management procedure, various DNA-based methods have formerly been used as complement to the traditional morphological methods. In the current study, a technique integrating loop-mediated isothermal amplification (LAMP) with PCR (polymerase chain reaction) was utilized for quick detection of T. urticae and discriminating it from other Tetranychus species attacking greenhouse crops. The assay was validated against various developmental stages of T. urticae specimens. Not only did the PCR-LAMP assay decrease the time needed for identification, but also was species-specific and sensitive enough to be applied even by non-specialists.

Keywords

Two-spotted spider mite Tetranychinae Major pest Molecular diagnosis LAMP 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11756_2018_187_MOESM1_ESM.pdf (110 kb)
ESM 1 (PDF 109 kb)
11756_2018_187_MOESM2_ESM.pdf (297 kb)
ESM 2 (PDF 296 kb)
11756_2018_187_MOESM3_ESM.pdf (197 kb)
ESM 3 (PDF 197 kb)

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Copyright information

© Institute of Zoology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Samira Sinaie
    • 1
  • Hussein Sadeghi-Namaghi
    • 1
    Email author
  • Lida Fekrat
    • 1
  1. 1.Department of Plant Protection, Faculty of AgricultureFerdowsi University of MashhadMashhadIran

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