, Volume 74, Issue 5, pp 463–467 | Cite as

Inhibition of Fasciola hepatica infection in Galba truncatula snails by application of monosaccharides to the aquatic environment

  • Katya GeorgievaEmail author
  • Petar Hristov
  • Neli Tsocheva-Gaytandzhieva
  • Veselin Nanev
Original Article


Fasciola hepatica is one of the etiological agents of fasciolosis, a widespread disease in domestic animals and occasionally in humans. Fasciolosis may be reduced by blocking the parasite transmission through its intermediate hosts. In the present work, different monosaccharides have been tested for their ability to impact on ligand/receptor interactions at the interface between the parasite and the intermediate host. Laboratory snails were subjected to miracidia in the presence of methyl-α-D-mannopyranoside (MetMan), α-D-glucose (Glc), N-acetyl-D-glucosamine (GlcNAc), D-(+)-galactose (Gal), N-acetyl-D-galactosamine (GalNAc) or L-(−)-fucose (Fuc), in 10 mM concentration. The snail survival rates and the prevalence of infection were determined after 50 days. Survived snails in the study groups varied from 78% to 97%. A remarkable reduction in the number of parasite-infected snails was observed in groups subjected to MetMan, Glc, or GlcNAc – 36.9%, 10.9%, and 11.9%, respectively, compared to 92.7% in the control group. Other tested monosaccharides had a low impact on snail infection. The results point to the implication that surface carbohydrate/receptor interactions are among the determining factors concerning the transmission of F. hepatica by the specific vector Galba truncatula. Biological recognition between the two organisms can be interfered with appropriate monosaccharides and this can be used to develop an alternative method for control of fasciolosis at the intermediate host level.


Fasciola hepatica Galba truncatula Lectin/carbohydtrate interactions Carbohydrate inhibition 



This study was funded by the National Science Fund of Ministry of Education and Science of the Republic of Bulgaria in contract No DN 01/3-16.12.2016.

Compliance with ethical standards

Ethical approval

All animals used in the experiments were handled in compliance with the Animal Protection Act of the Ministry of Agriculture and Food of the Republic of Bulgaria for the care and use of laboratory animals, and the study was approved by the local ethical committee.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Adema CM, Loker ES (2015) Digenean-gastropod host associations inform on aspects of specific immunity in snails. Dev Comp Immunol 48:275–283. CrossRefGoogle Scholar
  2. Andrews SJ (1999) The life cycle of Fasciola hepatica. In: Dalton JP (ed) Fasciolosis. CABI, Oxon, pp 1–29Google Scholar
  3. Bargues MD, Vigo M, Horak P et al. (2001) European Lymnaeidae (Mollusca: Gastropoda), intermediate hosts of trematodiases, based on nuclear ribosomal DNA ITS-2 sequences. Infect Genet Evol 1:85–107.
  4. Georgieva K, Georgieva S, Mizinska Y, Stoitsova SR (2012) Fasciola hepatica miracidia: lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation. Acta Parasitol 57:46–52. CrossRefGoogle Scholar
  5. Georgieva K, Georgieva L, Mizinska-Boevska Y, Stoitsova SR (2016) Study of surface carbohydrates in Galba truncatula tissues before and after infection with Fasciola hepatica. Mem Inst Oswaldo Cruz 111(7):475–483. CrossRefGoogle Scholar
  6. Georgieva K, Mizinska-Boevska Y, Stoitsova SR (2014) Localisation of galactose residues in the surface coat of Fasciola hepatica miracidia. C R Acad Bulg Sci 67:251–254Google Scholar
  7. Gordon D, Zadoks R, Skuce P, Sargison N (2012) Confirmation of triclabendazole resistance in liver fluke in the UK. Vet Rec 171(6):159–160. CrossRefGoogle Scholar
  8. Haas W (2003) Parasitic worms: strategies of host finding, recognition and invasion. Zoology 106:349–364. CrossRefGoogle Scholar
  9. Iguchi SM, Momoi T, Egawa K, Matsumoto JJ (1985) An N-acetylneuraminic acid-specific lectin from the body surface mucus of African giant snail. Comp Biochem Physiol (B) 81(4):897–900. Google Scholar
  10. Ito S, Shimizu M, Nagatsuka M, Kitajima S, Honda M, Tsuchiya T, Kanzawa N (2011) High molecular weight lectin isolated from the mucus of the giant African snail Achatina fulica. Biosci Biotechnol Biochem 75:20–25. CrossRefGoogle Scholar
  11. Kalbe M, Haberl B, Haas W (2000) Snail host finding by Fasciola hepatica and Trichobilharzia ocellata: compound analysis of "miracidia-attracting glycoproteins". Exp Parasitol 96:231–242. CrossRefGoogle Scholar
  12. Kelley JM,  Elliott TP, Beddoe T et al. (2016) Current threat of triclabendazole resistance in Fasciola hepatica. Trends Parasitol 32:458–469.
  13. Køie M, Christensen NØ, Nansen P (1976) Stereoscan studies of eggs, free-swimming and penetrating miracidia and early sporocysts of Fasciola hepatica. Z F Parasitenkunde 51:79–90. CrossRefGoogle Scholar
  14. Lehr T, Beuerlein K, Doenhoff MJ, Grevelding CG, Geyer R (2008) Localization of carbohydrate determinants common to Biomphalaria glabrata as well as to sporocysts and miracidia of Schistosoma mansoni. Parasitology 135:931–942. CrossRefGoogle Scholar
  15. Mas-Coma S, Bargues MD, Valero MA (2018) Human fascioliasis infection, sources, their diversity, incidence factors, analytical methods and prevention measures. Parasitology 145:1665–1699. CrossRefGoogle Scholar
  16. Mehmood K, Zhang H, Sabir AJ et al. (2017) A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants. Microb Pathog 109:253–262.
  17. Pales Espinosa E, Perrigault M, Ward JE, Shumway SE, Allam B (2009) Lectins associated with the feeding organs of the oyster Crassostrea virginica can mediate particle selection. Biol Bull 217:130–141. CrossRefGoogle Scholar
  18. WHO (2012) Multicriteria-based ranking for risk management of food-borne parasites: report of a Joint FAO/Expert Meeting, 3-7 September 2012. FAO Headquarters, Rome.Google Scholar
  19. Yoshino TP, Coustau C (2011) Immunobiology of Biomphalaria–trematode interactions. In: Toledo R, Fried B (eds) Biomphalaria snails and larval trematodes. Springer, New York, pp 159–189.

Copyright information

© Institute of Zoology, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Katya Georgieva
    • 1
    Email author
  • Petar Hristov
    • 1
  • Neli Tsocheva-Gaytandzhieva
    • 2
  • Veselin Nanev
    • 2
  1. 1.Department of Animal Diversity and Resources, Institute of Biodiversity and Ecosystem ResearchBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of Experimental Parasitology, Institute of Experimental Morphology, Pathology and Anthropology with MuseumBulgarian Academy of SciencesSofiaBulgaria

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