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Biologia

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Potential production of polyphenols, carotenoids and glycoalkaloids in Solanum villosum Mill. under salt stress

  • Saoussen Ben-Abdallah
  • Walid Zorrig
  • Lisa Amyot
  • Justin Renaud
  • Abdelali Hannoufa
  • Mokhtar Lachâal
  • Najoua Karray-Bouraoui
Original Article
  • 16 Downloads

Abstract

Changes in the antioxidant defence systems were investigated in many aromatic and medicinal plants grown under salinity; however, no study is available about its effects on Solanum villosum Mill. In this study, we investigated the response of S. villosum to sodium chloride (NaCl), especially the impacts of NaCl on secondary metabolites production (phenolic compounds, carotenoids, and glycol-alkaloids). For this purpose, a hydroponic culture experiment was conducted on seedlings subjected to 0, 50, 100, or 150 mM NaCl. NaCl affected the growth of S. villosum in terms of plant height and dry weight, however, it increased the water content of the leaves. Although leaf Na+ concentration was significantly increased under salt stress, the leaves K+, Ca2+ and Mg2+ concentration were enhanced and/ or not significantly affected. The adaptation of S. villosum to NaCl stress was also reflected by the maintain of chlorophyll levels under different NaCl treatments. Additionally, leaf caffeic acid, lutein, and beta-carotene contents were considerably increased under 100 mM NaCl treatment, along with the up regulation of some related phenolic (phenylalanine ammonialyase and flavonol synthase), and carotenoid genes (phytoene synthase 1, phytoene synthase 2, and b-lycopene cyclase). The leaf contents of β-solamargine and α-solasonine also increased significantly with increasing salinity. The present study is the first to through some light on the medicinal quality of S. villosum that would be quite helpful for production of secondary metabolites under salt-stressed conditions.

Keywords

Salinity Solanum villosum Phenolic compounds Carotenoid Gene expression 

Abbreviations

DW

Dry weight

Chl

Chlorophyll

MDA

Malondialdehyde

HPLC

High performance liquid chromatography

TPC

Total phenolic concentration

TFC

Total flavonoid concentration

TAC

Total antioxidant capacity

PCR

Polymerase chain reaction

qRT-PCR

Quantitative real time- polymerase chain reaction

Sv

Solanum villosum

PAL

Phenylalanine ammonialyase

CHS

Chalcone synthase

FLS

Flavonol synthase

PSY1

Phytoene synthase 1

PSY2

Phytoene synthase 2

β-LCY

b-lycopene cyclase

PAC

Principal Component Analysis

Notes

Acknowledgments

Authors listed, have made direct and intellectual contributions to the manuscript, and approved it for publication. S.B.A. was a visiting student at Agriculture and Agri-Food Research Centre in London, Ontario. The work was supported by the Ministry of Higher Education and Scientific Research of Tunisia.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Molecular Biology, Slovak Academy of Sciences 2018

Authors and Affiliations

  1. 1.Laboratoire de productivité végétale et contraintes environnementales, Département de Biologie. Faculté des Sciences de TunisUniversite de Tunis El ManarTunis El ManarTunisia
  2. 2.Agriculture and Agri-Food CanadaLondonCanada
  3. 3.Laboratory of Extremophile PlantsCentre of Biotechnology of Borj-CedriaHammam-LifTunisia

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