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Biologia

, Volume 74, Issue 3, pp 257–267 | Cite as

Molecular and morphological variation of the root-lesion nematode Pratylenchus neglectus

  • Nafiseh Divsalar
  • Ebrahim ShokoohiEmail author
  • Akbar Hoseinipour
  • Phatu Mashela
Original Article
  • 113 Downloads

Abstract

During a survey of plant-parasitic nematodes in Iran, five populations of Pratylenchus neglectus were studied by means of morphological and molecular characterization. The results showed that body length has high significant correlation with morphometric data indices such as a (r = 0.487; p ≤ 0.01), b (r = 0.355; p ≤ 0.05), c (r = 0.527; p ≤ 0.01), stylet (r = 0.625; p ≤ 0.01), excretory pore (r = 0.750; p ≤ 0.01), overlapping (r = 0.434; p ≤ 0.01) and tail length (r = 0.447; p ≤ 0.05). However, the highest correlation was detected between pharynx overlapping and postvulval uterine sac (r = −0.935; p ≤ 0.01). A molecular study of the P. neglectus populations using 28S rDNA showed close relationships of the Iranian, UK (KX683377), South Korea (KY468842) and China (JX046968). The COI of mtDNA analysis showed close relationship of the Iranian P. neglectus with the Chineese populations (KX349423; KY424105). The results indicated monophyly of P. neglectus. Illustration and measurement tables are provided for the species.

Keywords

Iran Morphometric Phylogeny Pratylenchus neglectus 28S rDNA Mitochondrial DNA 

Notes

Acknowledgments

The authors acknowledge the University of Limpopo of South Africa for supporting the project.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Institute of Zoology, Slovak Academy of Sciences 2018

Authors and Affiliations

  • Nafiseh Divsalar
    • 1
  • Ebrahim Shokoohi
    • 2
    Email author
  • Akbar Hoseinipour
    • 1
  • Phatu Mashela
    • 2
  1. 1.Department of Plant Protection, College of AgricultureShahid Bahonar University of KermanKermanIran
  2. 2.Green Biotechnologies Research Centre of ExcellenceUniversity of LimpopoSovengaSouth Africa

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