Development and Evaluation of Modified Cryopreservation for Long-Term Storage of Blastocystis Subtypes 1–3 and 6

  • Seyed Ahmad Karamati
  • Hamid Asadzadeh Aghdaei
  • Maryam NiyyatiEmail author
  • Abbas Yadegar
  • Ali Haghighi
  • Seyyed Javad Seyyed Tabaei
  • Hamed MirjalaliEmail author
  • Mohammad Reza Zali
Short Communication



Blastocystis is a protozoan parasite living in the intestine of humans and a wide range of animals. Although Blastocystis grows in several cultivation media, axenification and serial cultivations for long time are the main challenges of the researchers. Therefore, the long-term storage of subtypes/strains of Blastocystis using cryopreservation provides a suitable source of this parasite for the physiological, biochemical, and biological studies.


In the current study, seven xenic isolates including two separated isolates from ST1-3 and one isolate from ST6 were cryopreserved using a standard method with minor modifications. After 3 months, all isolates were recovered and cultivated in DMEM medium.


The findings of the method showed all seven isolates were successfully recovered in DMEM medium. In addition, all isolates remained viable after several sub-cultures.


It seems that cryopreservation is a simple method that can provide a suitable condition for the long-term storage of Blastocystis.


Blastocystis Subtypes Cryopreservation Long-term storage 



This article has been extracted from the thesis written by Mr. Seyed Ahmad Karamati in School of Medicine, Shahid Beheshti University of Medical Science (Registration No: M341). The project was also financially supported by Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences (grant No. 887). The authors would like to thank all the staffs of the Foodborne and Waterborne Diseases Research Center for their collaboration.

Author contributions

Conceived and designed the experiments: HM SAK MN. Performed the experiments: HM SAK. Analyzed the data: HM SAK. Contributed reagents/materials/analysis tools/positive samples: MN AH HAA MRZ. Wrote the paper: HM SAK SJST. All authors read and approved the final version of the manuscript.


This study was financially supported by the Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran (grant no. 887).

Compliance with ethical standards

Conflict of interests

The authors declare that they have no competing interests.

Ethics approval and consent to participate

This study did not use any human participants, human data or human tissue and, therefore, did not require ethical approval.

Informed consent

This study did not use any individual person’s data in any form and, therefore, did not require consent for publication.


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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2020

Authors and Affiliations

  1. 1.Department of Medical Parasitology and Mycology, Faculty of MedicineShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesTehranIran
  4. 4.Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesTehranIran

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